Basics of Antibody Phage Display Technology
Antibody discovery has become increasingly important in almost all areas of modern medicine. Different antibody discovery approaches exist, but one that has gained increasing interest in the field of toxinology and antivenom research is phage display technology. In this review, the lifecycle of the...
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doaj-f3634a69c99d47e885bdfe0f432258da2020-11-25T01:41:03ZengMDPI AGToxins2072-66512018-06-0110623610.3390/toxins10060236toxins10060236Basics of Antibody Phage Display TechnologyLine Ledsgaard0Mogens Kilstrup1Aneesh Karatt-Vellatt2John McCafferty3Andreas H. Laustsen4Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby DK-2800, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby DK-2800, DenmarkIONTAS Ltd., Cambridgeshire CB22 3EG, United KingdomIONTAS Ltd., Cambridgeshire CB22 3EG, United KingdomDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby DK-2800, DenmarkAntibody discovery has become increasingly important in almost all areas of modern medicine. Different antibody discovery approaches exist, but one that has gained increasing interest in the field of toxinology and antivenom research is phage display technology. In this review, the lifecycle of the M13 phage and the basics of phage display technology are presented together with important factors influencing the success rates of phage display experiments. Moreover, the pros and cons of different antigen display methods and the use of naïve versus immunized phage display antibody libraries is discussed, and selected examples from the field of antivenom research are highlighted. This review thus provides in-depth knowledge on the principles and use of phage display technology with a special focus on discovery of antibodies that target animal toxins.http://www.mdpi.com/2072-6651/10/6/236antibody discoveryrecombinant antivenomphage displayM13 phagetoxinology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Line Ledsgaard Mogens Kilstrup Aneesh Karatt-Vellatt John McCafferty Andreas H. Laustsen |
spellingShingle |
Line Ledsgaard Mogens Kilstrup Aneesh Karatt-Vellatt John McCafferty Andreas H. Laustsen Basics of Antibody Phage Display Technology Toxins antibody discovery recombinant antivenom phage display M13 phage toxinology |
author_facet |
Line Ledsgaard Mogens Kilstrup Aneesh Karatt-Vellatt John McCafferty Andreas H. Laustsen |
author_sort |
Line Ledsgaard |
title |
Basics of Antibody Phage Display Technology |
title_short |
Basics of Antibody Phage Display Technology |
title_full |
Basics of Antibody Phage Display Technology |
title_fullStr |
Basics of Antibody Phage Display Technology |
title_full_unstemmed |
Basics of Antibody Phage Display Technology |
title_sort |
basics of antibody phage display technology |
publisher |
MDPI AG |
series |
Toxins |
issn |
2072-6651 |
publishDate |
2018-06-01 |
description |
Antibody discovery has become increasingly important in almost all areas of modern medicine. Different antibody discovery approaches exist, but one that has gained increasing interest in the field of toxinology and antivenom research is phage display technology. In this review, the lifecycle of the M13 phage and the basics of phage display technology are presented together with important factors influencing the success rates of phage display experiments. Moreover, the pros and cons of different antigen display methods and the use of naïve versus immunized phage display antibody libraries is discussed, and selected examples from the field of antivenom research are highlighted. This review thus provides in-depth knowledge on the principles and use of phage display technology with a special focus on discovery of antibodies that target animal toxins. |
topic |
antibody discovery recombinant antivenom phage display M13 phage toxinology |
url |
http://www.mdpi.com/2072-6651/10/6/236 |
work_keys_str_mv |
AT lineledsgaard basicsofantibodyphagedisplaytechnology AT mogenskilstrup basicsofantibodyphagedisplaytechnology AT aneeshkarattvellatt basicsofantibodyphagedisplaytechnology AT johnmccafferty basicsofantibodyphagedisplaytechnology AT andreashlaustsen basicsofantibodyphagedisplaytechnology |
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1725042805637644288 |