Laboratory and Field Testing of an Automated Atmospheric Particle-Bound Reactive Oxygen Species Sampling-Analysis System
In this study, various laboratory and field tests were performed to develop an effective automated particle-bound ROS sampling-analysis system. The system uses 2′ 7′-dichlorofluorescin (DCFH) fluorescence method as a nonspecific, general indicator of the particle-bound ROS. A sharp-cut cyclone and a...
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doaj-f3ac54ee880342efacec0383c452077c2020-11-24T20:45:44ZengHindawi LimitedJournal of Toxicology1687-81911687-82052011-01-01201110.1155/2011/419476419476Laboratory and Field Testing of an Automated Atmospheric Particle-Bound Reactive Oxygen Species Sampling-Analysis SystemYungang Wang0Philip K. Hopke1Liping Sun2David C. Chalupa3Mark J. Utell4Center for Air Resource Engineering and Science, Clarkson University, Potsdam, NY 13699-5708, USACenter for Air Resource Engineering and Science, Clarkson University, Potsdam, NY 13699-5708, USACenter for Air Resource Engineering and Science, Clarkson University, Potsdam, NY 13699-5708, USADepartment of Environmental Medicine, University of Rochester Medical Center, Rochester, NY 14627, USADepartment of Environmental Medicine, University of Rochester Medical Center, Rochester, NY 14627, USAIn this study, various laboratory and field tests were performed to develop an effective automated particle-bound ROS sampling-analysis system. The system uses 2′ 7′-dichlorofluorescin (DCFH) fluorescence method as a nonspecific, general indicator of the particle-bound ROS. A sharp-cut cyclone and a particle-into-liquid sampler (PILS) were used to collect PM2.5 atmospheric particles into slurry produced by a DCFH-HRP solution. The laboratory results show that the DCFH and H2O2 standard solutions could be kept at room temperature for at least three and eight days, respectively. The field test in Rochester, NY, shows that the average ROS concentration was 8.3±2.2 nmol of equivalent H2O2 m−3 of air. The ROS concentrations were observed to be greater after foggy conditions. This study demonstrates the first practical automated sampling-analysis system to measure this ambient particle component.http://dx.doi.org/10.1155/2011/419476 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yungang Wang Philip K. Hopke Liping Sun David C. Chalupa Mark J. Utell |
spellingShingle |
Yungang Wang Philip K. Hopke Liping Sun David C. Chalupa Mark J. Utell Laboratory and Field Testing of an Automated Atmospheric Particle-Bound Reactive Oxygen Species Sampling-Analysis System Journal of Toxicology |
author_facet |
Yungang Wang Philip K. Hopke Liping Sun David C. Chalupa Mark J. Utell |
author_sort |
Yungang Wang |
title |
Laboratory and Field Testing of an Automated Atmospheric Particle-Bound Reactive Oxygen Species Sampling-Analysis System |
title_short |
Laboratory and Field Testing of an Automated Atmospheric Particle-Bound Reactive Oxygen Species Sampling-Analysis System |
title_full |
Laboratory and Field Testing of an Automated Atmospheric Particle-Bound Reactive Oxygen Species Sampling-Analysis System |
title_fullStr |
Laboratory and Field Testing of an Automated Atmospheric Particle-Bound Reactive Oxygen Species Sampling-Analysis System |
title_full_unstemmed |
Laboratory and Field Testing of an Automated Atmospheric Particle-Bound Reactive Oxygen Species Sampling-Analysis System |
title_sort |
laboratory and field testing of an automated atmospheric particle-bound reactive oxygen species sampling-analysis system |
publisher |
Hindawi Limited |
series |
Journal of Toxicology |
issn |
1687-8191 1687-8205 |
publishDate |
2011-01-01 |
description |
In this study, various laboratory and field tests were performed to develop an effective automated particle-bound ROS sampling-analysis system. The system uses 2′ 7′-dichlorofluorescin (DCFH) fluorescence method as a nonspecific, general indicator of the particle-bound ROS. A sharp-cut cyclone and a particle-into-liquid sampler (PILS) were used to collect PM2.5 atmospheric particles into slurry produced by a DCFH-HRP solution. The laboratory results show that the DCFH and H2O2 standard solutions could be kept at room temperature for at least three and eight days, respectively. The field test in Rochester, NY, shows that the average ROS concentration was 8.3±2.2 nmol of equivalent H2O2 m−3 of air. The ROS concentrations were observed to be greater after foggy conditions. This study demonstrates the first practical automated sampling-analysis system to measure this ambient particle component. |
url |
http://dx.doi.org/10.1155/2011/419476 |
work_keys_str_mv |
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