Overexpression of AtEDT1/HDG11 in Chinese kale (Brassica oleracea var. alboglabra) enhances drought and osmotic stress tolerance
Plants are constantly challenged by environmental stresses, including drought and high salinity. Improvement of drought and osmotic stress tolerance without yield decrease has been a great challenge in crop improvement. The Arabidopsis ENHANCED DROUGHT TOLERANCE1/HOMEODOMAIN GLABROUS11 (AtEDT1/HDG11...
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doaj-f3ac9065b59c4b5cba20384db6ca8d642020-11-25T00:35:48ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-08-01710.3389/fpls.2016.01285209483Overexpression of AtEDT1/HDG11 in Chinese kale (Brassica oleracea var. alboglabra) enhances drought and osmotic stress toleranceZhangsheng Zhu0Binmei Sun1Xiaoxia Xu2Hao Chen3Lifang Zou4Guoju Chen5Bihao Cao6Changming Chen7Jianjun Lei8South China Agriculture UniversitySouth China Agriculture UniversitySouth China Agriculture UniversitySouth China Agriculture UniversitySouth China Agriculture UniversitySouth China Agriculture UniversitySouth China Agriculture UniversitySouth China Agriculture UniversitySouth China Agriculture UniversityPlants are constantly challenged by environmental stresses, including drought and high salinity. Improvement of drought and osmotic stress tolerance without yield decrease has been a great challenge in crop improvement. The Arabidopsis ENHANCED DROUGHT TOLERANCE1/HOMEODOMAIN GLABROUS11 (AtEDT1/HDG11), a protein of the class IV HD-Zip family, has been demonstrated to significantly improve drought tolerance in Arabidopsis, rice and pepper. Here, we report that AtEDT1/HDG11 confers drought and osmotic stress tolerance in the Chinese kale. AtEDT1/HDG11-overexpression lines exhibit auxin-overproduction phenotypes, such as long hypocotyls, tall stems, more root hairs and a larger root system architecture. Compared with the untransformed control, transgenic lines have significantly reduced stomatal density. In the leaves of transgenic Chinese kale plants, proline (Pro) content and reactive oxygen species-scavenging enzyme activity was significantly increased after drought and osmotic stress, particularly compared to wild kale. More importantly, AtEDT1/HDG11-overexpression leads to abscisic acid (ABA) hypersensitivity, resulting in ABA inhibitor germination and induced stomatal closure. Consistent with observed phenotypes, the expression levels of auxin, ABA and stress-related genes were also altered under both normal and/or stress conditions. Further analysis showed that AtEDT1/HDG11, as a transcription factor, can target the auxin biosynthesis gene YUCC6 and ABA response genes ABI3 and ABI5. Collectively, our results provide a new insight into the role of AtEDT1/HDG11 in enhancing abiotic stress resistance through auxin- and ABA- mediated signaling response in Chinese kale.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01285/fullAbscisic Aciddroughtauxinosmotic stressAtEDT1/HDG11signaling response |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhangsheng Zhu Binmei Sun Xiaoxia Xu Hao Chen Lifang Zou Guoju Chen Bihao Cao Changming Chen Jianjun Lei |
spellingShingle |
Zhangsheng Zhu Binmei Sun Xiaoxia Xu Hao Chen Lifang Zou Guoju Chen Bihao Cao Changming Chen Jianjun Lei Overexpression of AtEDT1/HDG11 in Chinese kale (Brassica oleracea var. alboglabra) enhances drought and osmotic stress tolerance Frontiers in Plant Science Abscisic Acid drought auxin osmotic stress AtEDT1/HDG11 signaling response |
author_facet |
Zhangsheng Zhu Binmei Sun Xiaoxia Xu Hao Chen Lifang Zou Guoju Chen Bihao Cao Changming Chen Jianjun Lei |
author_sort |
Zhangsheng Zhu |
title |
Overexpression of AtEDT1/HDG11 in Chinese kale (Brassica oleracea var. alboglabra) enhances drought and osmotic stress tolerance |
title_short |
Overexpression of AtEDT1/HDG11 in Chinese kale (Brassica oleracea var. alboglabra) enhances drought and osmotic stress tolerance |
title_full |
Overexpression of AtEDT1/HDG11 in Chinese kale (Brassica oleracea var. alboglabra) enhances drought and osmotic stress tolerance |
title_fullStr |
Overexpression of AtEDT1/HDG11 in Chinese kale (Brassica oleracea var. alboglabra) enhances drought and osmotic stress tolerance |
title_full_unstemmed |
Overexpression of AtEDT1/HDG11 in Chinese kale (Brassica oleracea var. alboglabra) enhances drought and osmotic stress tolerance |
title_sort |
overexpression of atedt1/hdg11 in chinese kale (brassica oleracea var. alboglabra) enhances drought and osmotic stress tolerance |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2016-08-01 |
description |
Plants are constantly challenged by environmental stresses, including drought and high salinity. Improvement of drought and osmotic stress tolerance without yield decrease has been a great challenge in crop improvement. The Arabidopsis ENHANCED DROUGHT TOLERANCE1/HOMEODOMAIN GLABROUS11 (AtEDT1/HDG11), a protein of the class IV HD-Zip family, has been demonstrated to significantly improve drought tolerance in Arabidopsis, rice and pepper. Here, we report that AtEDT1/HDG11 confers drought and osmotic stress tolerance in the Chinese kale. AtEDT1/HDG11-overexpression lines exhibit auxin-overproduction phenotypes, such as long hypocotyls, tall stems, more root hairs and a larger root system architecture. Compared with the untransformed control, transgenic lines have significantly reduced stomatal density. In the leaves of transgenic Chinese kale plants, proline (Pro) content and reactive oxygen species-scavenging enzyme activity was significantly increased after drought and osmotic stress, particularly compared to wild kale. More importantly, AtEDT1/HDG11-overexpression leads to abscisic acid (ABA) hypersensitivity, resulting in ABA inhibitor germination and induced stomatal closure. Consistent with observed phenotypes, the expression levels of auxin, ABA and stress-related genes were also altered under both normal and/or stress conditions. Further analysis showed that AtEDT1/HDG11, as a transcription factor, can target the auxin biosynthesis gene YUCC6 and ABA response genes ABI3 and ABI5. Collectively, our results provide a new insight into the role of AtEDT1/HDG11 in enhancing abiotic stress resistance through auxin- and ABA- mediated signaling response in Chinese kale. |
topic |
Abscisic Acid drought auxin osmotic stress AtEDT1/HDG11 signaling response |
url |
http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01285/full |
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