Effect of inhibiting HMGB1 gene expression on ultrasonic blood flow characteristics, inflammatory response and apoptosis in diabetic retinopathy rats
Objective: To study the effect of inhibiting high mobility group box 1 (HMGB1) gene expression on ultrasonic blood flow characteristics, inflammatory response and apoptosis in diabetic retinopathy rats. Methods: Male SD rats were chosen and randomly divided into control group, model group and HMG...
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doaj-f3adad4b06e24b5f8f099c1be4e2390f2020-11-25T03:49:33ZengEditorial Board of Journal of Hainan Medical UniversityJournal of Hainan Medical University1007-12371007-12372020-04-012681619Effect of inhibiting HMGB1 gene expression on ultrasonic blood flow characteristics, inflammatory response and apoptosis in diabetic retinopathy ratsYun-Feng Wei0Qian Ding1Department of Ultrasound Medicine, Mianyang Central Hospital in Sichuan Province, Mianyang, Sichuan Province, 621000Department of Ophthalmology, Mianyang Central Hospital in Sichuan Province, Mianyang, Sichuan Province, 621000Objective: To study the effect of inhibiting high mobility group box 1 (HMGB1) gene expression on ultrasonic blood flow characteristics, inflammatory response and apoptosis in diabetic retinopathy rats. Methods: Male SD rats were chosen and randomly divided into control group, model group and HMGB1 inhibition group, the latter two groups were given intraperitoneal injection of streptozotocin to establish the diabetes model, and HMGB1 group were given tail vein injection of HMGB1-shRNA lentivirus for intervention. The peak endsystolic velocity (PSV), peak end-diastolic velocity (EDV), resistance index (RI) and pulsatility index (PI) of central retinal artery as well as the HMGB1, bcl-2, bax and cleaved caspase-3 expression and TNF-α, MCP-1 and ICAM-1 contents in retinal tissues were measured at 4 weeks after intervention. Results: The PSV and EDV levels as well as bcl-2 expression in the model group were lower than those in the control group, while the RI and PI levels as well as HMGB1, bax and cleaved caspase-3 expression and TNF-α, MCP-1 and ICAM-1 contents were higher than those in the control group; the PSV and EDV levels as well as bcl-2 expression in the HMGB1 inhibition group were higher than those in the model group, while the RI and PI levels as well as HMGB1, bax and cleaved caspase-3 expression and TNF-α, MCP-1 and ICAM-1 contents were lower than those in the model group. Conclusion: Inhibition of HMGB1 gene expression can improve the blood flow of central retinal artery and inhibit the inflammatory response and apoptosis in diabetic retinopathy rats.http://www.hnykdxxb.com/PDF/202008/04.pdfdiabetic retinopathyhigh mobility group box 1inflammatory responseapoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yun-Feng Wei Qian Ding |
spellingShingle |
Yun-Feng Wei Qian Ding Effect of inhibiting HMGB1 gene expression on ultrasonic blood flow characteristics, inflammatory response and apoptosis in diabetic retinopathy rats Journal of Hainan Medical University diabetic retinopathy high mobility group box 1 inflammatory response apoptosis |
author_facet |
Yun-Feng Wei Qian Ding |
author_sort |
Yun-Feng Wei |
title |
Effect of inhibiting HMGB1 gene expression on ultrasonic blood flow characteristics, inflammatory response and apoptosis in diabetic retinopathy rats |
title_short |
Effect of inhibiting HMGB1 gene expression on ultrasonic blood flow characteristics, inflammatory response and apoptosis in diabetic retinopathy rats |
title_full |
Effect of inhibiting HMGB1 gene expression on ultrasonic blood flow characteristics, inflammatory response and apoptosis in diabetic retinopathy rats |
title_fullStr |
Effect of inhibiting HMGB1 gene expression on ultrasonic blood flow characteristics, inflammatory response and apoptosis in diabetic retinopathy rats |
title_full_unstemmed |
Effect of inhibiting HMGB1 gene expression on ultrasonic blood flow characteristics, inflammatory response and apoptosis in diabetic retinopathy rats |
title_sort |
effect of inhibiting hmgb1 gene expression on ultrasonic blood flow characteristics, inflammatory response and apoptosis in diabetic retinopathy rats |
publisher |
Editorial Board of Journal of Hainan Medical University |
series |
Journal of Hainan Medical University |
issn |
1007-1237 1007-1237 |
publishDate |
2020-04-01 |
description |
Objective: To study the effect of inhibiting high mobility group box 1 (HMGB1) gene
expression on ultrasonic blood flow characteristics, inflammatory response and apoptosis in
diabetic retinopathy rats. Methods: Male SD rats were chosen and randomly divided into
control group, model group and HMGB1 inhibition group, the latter two groups were given
intraperitoneal injection of streptozotocin to establish the diabetes model, and HMGB1 group
were given tail vein injection of HMGB1-shRNA lentivirus for intervention. The peak endsystolic
velocity (PSV), peak end-diastolic velocity (EDV), resistance index (RI) and pulsatility
index (PI) of central retinal artery as well as the HMGB1, bcl-2, bax and cleaved caspase-3
expression and TNF-α, MCP-1 and ICAM-1 contents in retinal tissues were measured at 4
weeks after intervention. Results: The PSV and EDV levels as well as bcl-2 expression in
the model group were lower than those in the control group, while the RI and PI levels as
well as HMGB1, bax and cleaved caspase-3 expression and TNF-α, MCP-1 and ICAM-1
contents were higher than those in the control group; the PSV and EDV levels as well as bcl-2
expression in the HMGB1 inhibition group were higher than those in the model group, while
the RI and PI levels as well as HMGB1, bax and cleaved caspase-3 expression and TNF-α,
MCP-1 and ICAM-1 contents were lower than those in the model group. Conclusion:
Inhibition of HMGB1 gene expression can improve the blood flow of central retinal artery and
inhibit the inflammatory response and apoptosis in diabetic retinopathy rats. |
topic |
diabetic retinopathy high mobility group box 1 inflammatory response apoptosis |
url |
http://www.hnykdxxb.com/PDF/202008/04.pdf |
work_keys_str_mv |
AT yunfengwei effectofinhibitinghmgb1geneexpressiononultrasonicbloodflowcharacteristicsinflammatoryresponseandapoptosisindiabeticretinopathyrats AT qianding effectofinhibitinghmgb1geneexpressiononultrasonicbloodflowcharacteristicsinflammatoryresponseandapoptosisindiabeticretinopathyrats |
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