Anxiolytic effects of repeated treatment with an essential oil from Lippia alba and (R)-(-)-carvone in the elevated T-maze
Lippia alba (Mill.) N.E. Brown (Verbenaceae) is widely used in different regions of Central and South America as a tranquilizer. The plant’s anxiolytic properties, however, merit investigation. The present study evaluated the effects of repeated daily (14 days) intraperitoneal (ip) treatme...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Associação Brasileira de Divulgação Científica
2012-03-01
|
Series: | Brazilian Journal of Medical and Biological Research |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300009 |
id |
doaj-f3b321f86ec540af9f77981f27d43eec |
---|---|
record_format |
Article |
spelling |
doaj-f3b321f86ec540af9f77981f27d43eec2020-11-24T20:49:11ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2012-03-01453238243Anxiolytic effects of repeated treatment with an essential oil from Lippia alba and (R)-(-)-carvone in the elevated T-mazeV.Y. HatanoA.S. TorricelliA.C.C. GiassiL.A. CoslopeM.B. VianaLippia alba (Mill.) N.E. Brown (Verbenaceae) is widely used in different regions of Central and South America as a tranquilizer. The plant’s anxiolytic properties, however, merit investigation. The present study evaluated the effects of repeated daily (14 days) intraperitoneal (ip) treatment with an essential oil (EO) from a chemotype of L. alba (LA, chemotype II, 12.5 and 25 mg/kg; N = 6-8) and (R)-(-)-carvone (25 mg/kg; N = 8-12), the main constituent of this chemotype, on male Wistar rats (weighing 250 g at the beginning of the experiments) submitted to the elevated T-maze (ETM). The ETM allows the measurement of two defensive responses: inhibitory avoidance and one-way escape. In terms of psychopathology, these responses have been related to generalized anxiety and panic disorder, respectively. Treatment with the EO impaired ETM avoidance latencies, without altering escape, in a way similar to the reference drug diazepam (P < 0.05) (avoidance 2: control = 84.6 ± 35.2; EO 12.5 mg/kg = 11.8 ± 3.8; EO 25 mg/kg = 14.6 ± 2.7; diazepam = 7 ± 2.1). (R)-(-)-carvone also significantly altered this same response (P < 0.05; avoidance 1: control = 91.9 ± 31.5; carvone = 11.6 ± 1.8; diazepam = 8.1 ± 3.3). These results were not due to motor changes since no significant effects were detected in an open field. These observations suggest that LA exerts anxiolytic-like effects on a specific subset of defensive behaviors that have been implicated in generalized anxiety disorder, and suggest that carvone is one of the constituents of LA responsible for its action as a tranquilizer.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300009Lippia albaEssential oilCarvoneAnxietyElevated T-maze |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
V.Y. Hatano A.S. Torricelli A.C.C. Giassi L.A. Coslope M.B. Viana |
spellingShingle |
V.Y. Hatano A.S. Torricelli A.C.C. Giassi L.A. Coslope M.B. Viana Anxiolytic effects of repeated treatment with an essential oil from Lippia alba and (R)-(-)-carvone in the elevated T-maze Brazilian Journal of Medical and Biological Research Lippia alba Essential oil Carvone Anxiety Elevated T-maze |
author_facet |
V.Y. Hatano A.S. Torricelli A.C.C. Giassi L.A. Coslope M.B. Viana |
author_sort |
V.Y. Hatano |
title |
Anxiolytic effects of repeated treatment with an essential oil from Lippia alba and (R)-(-)-carvone in the elevated T-maze |
title_short |
Anxiolytic effects of repeated treatment with an essential oil from Lippia alba and (R)-(-)-carvone in the elevated T-maze |
title_full |
Anxiolytic effects of repeated treatment with an essential oil from Lippia alba and (R)-(-)-carvone in the elevated T-maze |
title_fullStr |
Anxiolytic effects of repeated treatment with an essential oil from Lippia alba and (R)-(-)-carvone in the elevated T-maze |
title_full_unstemmed |
Anxiolytic effects of repeated treatment with an essential oil from Lippia alba and (R)-(-)-carvone in the elevated T-maze |
title_sort |
anxiolytic effects of repeated treatment with an essential oil from lippia alba and (r)-(-)-carvone in the elevated t-maze |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
0100-879X 1414-431X |
publishDate |
2012-03-01 |
description |
Lippia alba (Mill.) N.E. Brown (Verbenaceae) is widely used in different regions of Central and South America as a tranquilizer. The plant’s anxiolytic properties, however, merit investigation. The present study evaluated the effects of repeated daily (14 days) intraperitoneal (ip) treatment with an essential oil (EO) from a chemotype of L. alba (LA, chemotype II, 12.5 and 25 mg/kg; N = 6-8) and (R)-(-)-carvone (25 mg/kg; N = 8-12), the main constituent of this chemotype, on male Wistar rats (weighing 250 g at the beginning of the experiments) submitted to the elevated T-maze (ETM). The ETM allows the measurement of two defensive responses: inhibitory avoidance and one-way escape. In terms of psychopathology, these responses have been related to generalized anxiety and panic disorder, respectively. Treatment with the EO impaired ETM avoidance latencies, without altering escape, in a way similar to the reference drug diazepam (P < 0.05) (avoidance 2: control = 84.6 ± 35.2; EO 12.5 mg/kg = 11.8 ± 3.8; EO 25 mg/kg = 14.6 ± 2.7; diazepam = 7 ± 2.1). (R)-(-)-carvone also significantly altered this same response (P < 0.05; avoidance 1: control = 91.9 ± 31.5; carvone = 11.6 ± 1.8; diazepam = 8.1 ± 3.3). These results were not due to motor changes since no significant effects were detected in an open field. These observations suggest that LA exerts anxiolytic-like effects on a specific subset of defensive behaviors that have been implicated in generalized anxiety disorder, and suggest that carvone is one of the constituents of LA responsible for its action as a tranquilizer. |
topic |
Lippia alba Essential oil Carvone Anxiety Elevated T-maze |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300009 |
work_keys_str_mv |
AT vyhatano anxiolyticeffectsofrepeatedtreatmentwithanessentialoilfromlippiaalbaandrcarvoneintheelevatedtmaze AT astorricelli anxiolyticeffectsofrepeatedtreatmentwithanessentialoilfromlippiaalbaandrcarvoneintheelevatedtmaze AT accgiassi anxiolyticeffectsofrepeatedtreatmentwithanessentialoilfromlippiaalbaandrcarvoneintheelevatedtmaze AT lacoslope anxiolyticeffectsofrepeatedtreatmentwithanessentialoilfromlippiaalbaandrcarvoneintheelevatedtmaze AT mbviana anxiolyticeffectsofrepeatedtreatmentwithanessentialoilfromlippiaalbaandrcarvoneintheelevatedtmaze |
_version_ |
1716806564696817664 |