Use of CHROMagar Candida for the presumptive identification of Candida species directly from clinical specimens in resource-limited settings
Introduction: Identification of yeast isolated from clinical specimens to the species level has become increasingly important. Ever-increasing numbers of immuno-suppressed patients, a widening range of recognized pathogens, and the discovery of resistance to antifungal drugs are contributing factors...
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2010-02-01
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doaj-f3beb8a8b73d4277ac3c48c7109e5a802020-11-24T23:38:32ZengTaylor & Francis GroupLibyan Journal of Medicine1819-63572010-02-01501610.3402/ljm.v5i0.2144Use of CHROMagar Candida for the presumptive identification of Candida species directly from clinical specimens in resource-limited settingsSayyada Ghufrana NadeemShazia Tabassum HakimShahana Urooj KazmiIntroduction: Identification of yeast isolated from clinical specimens to the species level has become increasingly important. Ever-increasing numbers of immuno-suppressed patients, a widening range of recognized pathogens, and the discovery of resistance to antifungal drugs are contributing factors to this necessity. Material and methods: A total of 487 yeast strains were studied for the primary isolation and presumptive identification, directly from clinical specimen. Efficacy of CHROMagar Candida has been evaluated with conventional methods including morphology on Corn meal–tween 80 agar and biochemical methods by using API 20 C AUX. Results: The result of this study shows that CHROMagar Candida can easily identify three species of Candida on the basis of colonial color and morphology, and accurately differentiate between them i.e. Candida albicans, Candida tropicalis, and Candida krusei. The specificity and sensitivity of CHROMagar Candida for C. albicans calculated as 99%, for C. tropicalis calculated as 98%, and C. krusei it is 100%. Conclusion: The data presented supports the use of CHROMagar Candida for the rapid identification of Candida species directly from clinical specimens in resource-limited settings, which could be very helpful in developing appropriate therapeutic strategy and management of patients. http://www.libyanjournalofmedicine.net/index.php/ljm/article/view/2144/5396CHROMagar Candidaresource-limited settingspresumptive identification |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sayyada Ghufrana Nadeem Shazia Tabassum Hakim Shahana Urooj Kazmi |
spellingShingle |
Sayyada Ghufrana Nadeem Shazia Tabassum Hakim Shahana Urooj Kazmi Use of CHROMagar Candida for the presumptive identification of Candida species directly from clinical specimens in resource-limited settings Libyan Journal of Medicine CHROMagar Candida resource-limited settings presumptive identification |
author_facet |
Sayyada Ghufrana Nadeem Shazia Tabassum Hakim Shahana Urooj Kazmi |
author_sort |
Sayyada Ghufrana Nadeem |
title |
Use of CHROMagar Candida for the presumptive identification of Candida species directly from clinical specimens in resource-limited settings |
title_short |
Use of CHROMagar Candida for the presumptive identification of Candida species directly from clinical specimens in resource-limited settings |
title_full |
Use of CHROMagar Candida for the presumptive identification of Candida species directly from clinical specimens in resource-limited settings |
title_fullStr |
Use of CHROMagar Candida for the presumptive identification of Candida species directly from clinical specimens in resource-limited settings |
title_full_unstemmed |
Use of CHROMagar Candida for the presumptive identification of Candida species directly from clinical specimens in resource-limited settings |
title_sort |
use of chromagar candida for the presumptive identification of candida species directly from clinical specimens in resource-limited settings |
publisher |
Taylor & Francis Group |
series |
Libyan Journal of Medicine |
issn |
1819-6357 |
publishDate |
2010-02-01 |
description |
Introduction: Identification of yeast isolated from clinical specimens to the species level has become increasingly important. Ever-increasing numbers of immuno-suppressed patients, a widening range of recognized pathogens, and the discovery of resistance to antifungal drugs are contributing factors to this necessity. Material and methods: A total of 487 yeast strains were studied for the primary isolation and presumptive identification, directly from clinical specimen. Efficacy of CHROMagar Candida has been evaluated with conventional methods including morphology on Corn meal–tween 80 agar and biochemical methods by using API 20 C AUX. Results: The result of this study shows that CHROMagar Candida can easily identify three species of Candida on the basis of colonial color and morphology, and accurately differentiate between them i.e. Candida albicans, Candida tropicalis, and Candida krusei. The specificity and sensitivity of CHROMagar Candida for C. albicans calculated as 99%, for C. tropicalis calculated as 98%, and C. krusei it is 100%. Conclusion: The data presented supports the use of CHROMagar Candida for the rapid identification of Candida species directly from clinical specimens in resource-limited settings, which could be very helpful in developing appropriate therapeutic strategy and management of patients. |
topic |
CHROMagar Candida resource-limited settings presumptive identification |
url |
http://www.libyanjournalofmedicine.net/index.php/ljm/article/view/2144/5396 |
work_keys_str_mv |
AT sayyadaghufrananadeem useofchromagarcandidaforthepresumptiveidentificationofcandidaspeciesdirectlyfromclinicalspecimensinresourcelimitedsettings AT shaziatabassumhakim useofchromagarcandidaforthepresumptiveidentificationofcandidaspeciesdirectlyfromclinicalspecimensinresourcelimitedsettings AT shahanauroojkazmi useofchromagarcandidaforthepresumptiveidentificationofcandidaspeciesdirectlyfromclinicalspecimensinresourcelimitedsettings |
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