Staphylococcus aureus colonization in contact hypersensitivity induces a shift in cutaneous cytokine milieu from a Th1- to a Th2-type profile
Background: Colonization of atopic dermatitis lesions with Staphylococcus aureus is a frequent phenomenon and may exacerbate inflammation of the skin. The aim of the present study was to determine the effect of S. aureus on T cell-mediated immune responses in contact hypersensitivity caused by a hap...
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doaj-f3efc0bcf89546e0832b6ef1a250f47e2020-11-24T23:49:55ZengElsevierAllergology International1323-89302003-01-0152419119810.1046/j.1323-8930.2003.00302.xStaphylococcus aureus colonization in contact hypersensitivity induces a shift in cutaneous cytokine milieu from a Th1- to a Th2-type profileMasumi Ueda0Fusako Okazaki1Hiroko Kanzaki2Joji Tada3Jirô Arata4Department of Dermatology, Okayama University Medical School, Okayama, JapanDepartment of Dermatology, Okayama University Medical School, Okayama, JapanDepartment of Dermatology, Okayama University Medical School, Okayama, JapanDivision of Dermatology, Okayama City Hospital, Okayama, JapanDepartment of Dermatology, Okayama University Medical School, Okayama, JapanBackground: Colonization of atopic dermatitis lesions with Staphylococcus aureus is a frequent phenomenon and may exacerbate inflammation of the skin. The aim of the present study was to determine the effect of S. aureus on T cell-mediated immune responses in contact hypersensitivity caused by a hapten or a protein with large molecule. Methods: Staphylococcus aureus or phosphate-buffered saline was inoculated on experimental contact dermatitis induced by 2,4,6-trinitro-1-chlorobenzene (TNCB) or house dust mite antigen. At various times after inoculation, the experimental lesions were examined histologically and immunohistochemically. Furthermore, the kinetics of cytokine patterns (interferon (IFN)-γ, interleukin (IL)-2, IL-4 and IL-5) in each lesion were analyzed by reverse transcription-polymerase chain reaction. Results: In TNCB-challenged lesions inoculated with S. aureus, the levels of IL-2 mRNA decreased; in contrast, mRNA levels of IL-4 and IL-5 were upregulated. In mite antigen-challenged lesions, IL-4 and IL-5 mRNA expression was detected throughout the period of the investigation, even without S. aureus inoculation. Interferon-γ mRNA expression in mite dermatitis without S. aureus inoculation was observed only in the later period and IL-2 mRNA expression in mite dermatitis with S. aureus was suppressed and observed much later than in the control group. Conclusions: Infestation of S. aureus on skin lesions in experimental contact hypersensitivity induces a shift in the immune reaction from a Th1- to a Th2-dominant response.http://www.sciencedirect.com/science/article/pii/S1323893015311667atopic dermatitisStaphylococcus aureusTh1 cellsTh2 cells |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Masumi Ueda Fusako Okazaki Hiroko Kanzaki Joji Tada Jirô Arata |
spellingShingle |
Masumi Ueda Fusako Okazaki Hiroko Kanzaki Joji Tada Jirô Arata Staphylococcus aureus colonization in contact hypersensitivity induces a shift in cutaneous cytokine milieu from a Th1- to a Th2-type profile Allergology International atopic dermatitis Staphylococcus aureus Th1 cells Th2 cells |
author_facet |
Masumi Ueda Fusako Okazaki Hiroko Kanzaki Joji Tada Jirô Arata |
author_sort |
Masumi Ueda |
title |
Staphylococcus aureus colonization in contact hypersensitivity induces a shift in cutaneous cytokine milieu from a Th1- to a Th2-type profile |
title_short |
Staphylococcus aureus colonization in contact hypersensitivity induces a shift in cutaneous cytokine milieu from a Th1- to a Th2-type profile |
title_full |
Staphylococcus aureus colonization in contact hypersensitivity induces a shift in cutaneous cytokine milieu from a Th1- to a Th2-type profile |
title_fullStr |
Staphylococcus aureus colonization in contact hypersensitivity induces a shift in cutaneous cytokine milieu from a Th1- to a Th2-type profile |
title_full_unstemmed |
Staphylococcus aureus colonization in contact hypersensitivity induces a shift in cutaneous cytokine milieu from a Th1- to a Th2-type profile |
title_sort |
staphylococcus aureus colonization in contact hypersensitivity induces a shift in cutaneous cytokine milieu from a th1- to a th2-type profile |
publisher |
Elsevier |
series |
Allergology International |
issn |
1323-8930 |
publishDate |
2003-01-01 |
description |
Background: Colonization of atopic dermatitis lesions with Staphylococcus aureus is a frequent phenomenon and may exacerbate inflammation of the skin. The aim of the present study was to determine the effect of S. aureus on T cell-mediated immune responses in contact hypersensitivity caused by a hapten or a protein with large molecule.
Methods: Staphylococcus aureus or phosphate-buffered saline was inoculated on experimental contact dermatitis induced by 2,4,6-trinitro-1-chlorobenzene (TNCB) or house dust mite antigen. At various times after inoculation, the experimental lesions were examined histologically and immunohistochemically. Furthermore, the kinetics of cytokine patterns (interferon (IFN)-γ, interleukin (IL)-2, IL-4 and IL-5) in each lesion were analyzed by reverse transcription-polymerase chain reaction.
Results: In TNCB-challenged lesions inoculated with S. aureus, the levels of IL-2 mRNA decreased; in contrast, mRNA levels of IL-4 and IL-5 were upregulated. In mite antigen-challenged lesions, IL-4 and IL-5 mRNA expression was detected throughout the period of the investigation, even without S. aureus inoculation. Interferon-γ mRNA expression in mite dermatitis without S. aureus inoculation was observed only in the later period and IL-2 mRNA expression in mite dermatitis with S. aureus was suppressed and observed much later than in the control group.
Conclusions: Infestation of S. aureus on skin lesions in experimental contact hypersensitivity induces a shift in the immune reaction from a Th1- to a Th2-dominant response. |
topic |
atopic dermatitis Staphylococcus aureus Th1 cells Th2 cells |
url |
http://www.sciencedirect.com/science/article/pii/S1323893015311667 |
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