Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania Nanotubes
Successful dental implants rely on stable osseointegration and soft-tissue integration. Titania nanotubes (TNTs) with a diameter of 100 nm could increase the mesenchymal stem cell response and simultaneously decrease Staphylococcus aureus adhesion. However, the interactions between the modified surf...
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Online Access: | http://dx.doi.org/10.1155/2020/5651780 |
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doaj-f41e43c642324180a671615db2ec63ed2020-11-25T03:47:18ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/56517805651780Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania NanotubesZhiqiang Xu0Yuqi He1Xiufeng Zeng2Xiuxia Zeng3Junhui Huang4Xi Lin5Jiang Chen6Department of Stomatology, Affiliated Hospital of Putian University, Putian, Fujian 351100, ChinaDepartment of Stomatology, Affiliated Hospital of Putian University, Putian, Fujian 351100, ChinaHanjiang Zeng Xiufeng Dental Clinic, Putian, Fujian 351117, ChinaDepartment of Stomatology, Putian Hanjiang Hospital, Putian, Fujian 351100, ChinaDepartment of Stomatology, Affiliated Hospital of Putian University, Putian, Fujian 351100, ChinaLaboratory of Electron Microscopy, Public Technology Service Center, Fujian Medical University, Fuzhou, Fujian 350002, ChinaDepartment of Oral Implantology, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou, Fujian 350002, ChinaSuccessful dental implants rely on stable osseointegration and soft-tissue integration. Titania nanotubes (TNTs) with a diameter of 100 nm could increase the mesenchymal stem cell response and simultaneously decrease Staphylococcus aureus adhesion. However, the interactions between the modified surface and surrounding soft tissues are still unknown. In the present study, we fully investigated the biological behavior of human gingival fibroblasts (HGFs) and the adhesion of Porphyromonas gingivalis (P. gingivalis). TNTs were synthesized on titanium (Ti) surfaces by electrochemical anodization at 10, 30, and 60 V, and the products were denoted as NT10, NT30, and NT60, respectively. NT10 (diameter: 30 nm) and NT30 (diameter: 100 nm) could enhance the HGF functions, such as cell attachment and proliferation and extracellular matrix- (ECM-) related gene expressions, with the latter showing higher enhancement. NT60 (diameter: 200 nm) clearly impaired cell adhesion and proliferation and ECM-related gene expressions. Bacterial adhesion on the TNTs decreased and reached the lowest value on NT30. Therefore, NT30 without pharmaceuticals can be used to substantially enhance the HGF response and reduce P. gingivalis adhesion to the utmost, thus demonstrating significant potential in the transgingival part of dental implants.http://dx.doi.org/10.1155/2020/5651780 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhiqiang Xu Yuqi He Xiufeng Zeng Xiuxia Zeng Junhui Huang Xi Lin Jiang Chen |
spellingShingle |
Zhiqiang Xu Yuqi He Xiufeng Zeng Xiuxia Zeng Junhui Huang Xi Lin Jiang Chen Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania Nanotubes BioMed Research International |
author_facet |
Zhiqiang Xu Yuqi He Xiufeng Zeng Xiuxia Zeng Junhui Huang Xi Lin Jiang Chen |
author_sort |
Zhiqiang Xu |
title |
Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania Nanotubes |
title_short |
Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania Nanotubes |
title_full |
Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania Nanotubes |
title_fullStr |
Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania Nanotubes |
title_full_unstemmed |
Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania Nanotubes |
title_sort |
enhanced human gingival fibroblast response and reduced porphyromonas gingivalis adhesion with titania nanotubes |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2020-01-01 |
description |
Successful dental implants rely on stable osseointegration and soft-tissue integration. Titania nanotubes (TNTs) with a diameter of 100 nm could increase the mesenchymal stem cell response and simultaneously decrease Staphylococcus aureus adhesion. However, the interactions between the modified surface and surrounding soft tissues are still unknown. In the present study, we fully investigated the biological behavior of human gingival fibroblasts (HGFs) and the adhesion of Porphyromonas gingivalis (P. gingivalis). TNTs were synthesized on titanium (Ti) surfaces by electrochemical anodization at 10, 30, and 60 V, and the products were denoted as NT10, NT30, and NT60, respectively. NT10 (diameter: 30 nm) and NT30 (diameter: 100 nm) could enhance the HGF functions, such as cell attachment and proliferation and extracellular matrix- (ECM-) related gene expressions, with the latter showing higher enhancement. NT60 (diameter: 200 nm) clearly impaired cell adhesion and proliferation and ECM-related gene expressions. Bacterial adhesion on the TNTs decreased and reached the lowest value on NT30. Therefore, NT30 without pharmaceuticals can be used to substantially enhance the HGF response and reduce P. gingivalis adhesion to the utmost, thus demonstrating significant potential in the transgingival part of dental implants. |
url |
http://dx.doi.org/10.1155/2020/5651780 |
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1715117365071446016 |