Two PC 12 Pheochromocytoma Lines Sealed in Hollow Fiber-Based Capsules Tonically Release L-Dopa In Vitro
Two PC12 cell-derived lines have been studied following encapsulation into polymer-based hollow fibers with respect to secreted catecholamines and their metabolites. Cellular encapsulation provides a chronic microperfusion environment within which basally secreted PC12 products can be readily measur...
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SAGE Publishing
1993-03-01
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Series: | Cell Transplantation |
Online Access: | https://doi.org/10.1177/096368979300200209 |
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doaj-f4424975658a41c39d6af1b9c7ad50d22020-11-25T03:33:14ZengSAGE PublishingCell Transplantation0963-68971555-38921993-03-01210.1177/096368979300200209Two PC 12 Pheochromocytoma Lines Sealed in Hollow Fiber-Based Capsules Tonically Release L-Dopa In VitroMark P. Lavoie0Meg Palmatier1Frank T. Gentile2Faith A. Kaplan3Deborah M. Fiore4Tyrone F. Hazlett5William J. Bell6Thomas R. Flanagan PhD7CytoTherapeutics, Inc., Providence, RI 02906, USACytoTherapeutics, Inc., Providence, RI 02906, USACytoTherapeutics, Inc., Providence, RI 02906, USACytoTherapeutics, Inc., Providence, RI 02906, USACytoTherapeutics, Inc., Providence, RI 02906, USACytoTherapeutics, Inc., Providence, RI 02906, USACytoTherapeutics, Inc., Providence, RI 02906, USACytoTherapeutics, Inc., Providence, RI 02906, USATwo PC12 cell-derived lines have been studied following encapsulation into polymer-based hollow fibers with respect to secreted catecholamines and their metabolites. Cellular encapsulation provides a chronic microperfusion environment within which basally secreted PC12 products can be readily measured. Encapsulated PC12 cells grown and held under the conditions specified in this report basally release amounts exceeding their total cellular stores of the dopamine precursor L-DOPA and the electrochemically active dopamine metabolites DOPAC and HVA during 45-min static incubations. Under these same conditions, these cells release less than 0.1% of their total cellular store of dopamine. Depolarizing incubations enhance dopamine secretion eightyfold and enhance secretion of L-DOPA, HVA, and DOPAC about twofold. The relative composition of products basally secreted differs between PC12-derived cell lines, and an inverse relationship exists between basal release of L-DOPA and total cellular store of dopamine. These results further indicate that selected PC12 cell lines are potentially a source of both dopamine and L-DOPA in therapeutic cellular replacement applications.https://doi.org/10.1177/096368979300200209 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mark P. Lavoie Meg Palmatier Frank T. Gentile Faith A. Kaplan Deborah M. Fiore Tyrone F. Hazlett William J. Bell Thomas R. Flanagan PhD |
spellingShingle |
Mark P. Lavoie Meg Palmatier Frank T. Gentile Faith A. Kaplan Deborah M. Fiore Tyrone F. Hazlett William J. Bell Thomas R. Flanagan PhD Two PC 12 Pheochromocytoma Lines Sealed in Hollow Fiber-Based Capsules Tonically Release L-Dopa In Vitro Cell Transplantation |
author_facet |
Mark P. Lavoie Meg Palmatier Frank T. Gentile Faith A. Kaplan Deborah M. Fiore Tyrone F. Hazlett William J. Bell Thomas R. Flanagan PhD |
author_sort |
Mark P. Lavoie |
title |
Two PC 12 Pheochromocytoma Lines Sealed in Hollow Fiber-Based Capsules Tonically Release L-Dopa In Vitro |
title_short |
Two PC 12 Pheochromocytoma Lines Sealed in Hollow Fiber-Based Capsules Tonically Release L-Dopa In Vitro |
title_full |
Two PC 12 Pheochromocytoma Lines Sealed in Hollow Fiber-Based Capsules Tonically Release L-Dopa In Vitro |
title_fullStr |
Two PC 12 Pheochromocytoma Lines Sealed in Hollow Fiber-Based Capsules Tonically Release L-Dopa In Vitro |
title_full_unstemmed |
Two PC 12 Pheochromocytoma Lines Sealed in Hollow Fiber-Based Capsules Tonically Release L-Dopa In Vitro |
title_sort |
two pc 12 pheochromocytoma lines sealed in hollow fiber-based capsules tonically release l-dopa in vitro |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
1993-03-01 |
description |
Two PC12 cell-derived lines have been studied following encapsulation into polymer-based hollow fibers with respect to secreted catecholamines and their metabolites. Cellular encapsulation provides a chronic microperfusion environment within which basally secreted PC12 products can be readily measured. Encapsulated PC12 cells grown and held under the conditions specified in this report basally release amounts exceeding their total cellular stores of the dopamine precursor L-DOPA and the electrochemically active dopamine metabolites DOPAC and HVA during 45-min static incubations. Under these same conditions, these cells release less than 0.1% of their total cellular store of dopamine. Depolarizing incubations enhance dopamine secretion eightyfold and enhance secretion of L-DOPA, HVA, and DOPAC about twofold. The relative composition of products basally secreted differs between PC12-derived cell lines, and an inverse relationship exists between basal release of L-DOPA and total cellular store of dopamine. These results further indicate that selected PC12 cell lines are potentially a source of both dopamine and L-DOPA in therapeutic cellular replacement applications. |
url |
https://doi.org/10.1177/096368979300200209 |
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