Study on the Extraction Technology of <i>Candida antarctica</i> Lipase B by Foam Separation
<i>Candida antarctica</i> Lipase B (CALB) has a wide range of applications in many fields. In this study, <i>Pichia pastoris</i> was used to express CALB for fermentation tank culture. Sodium dodecyl sulfate (SDS) was used as a surfactant, and foam separation technology was u...
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doaj-f4560239d80647b4be02c19baadafe3d2020-12-24T00:03:53ZengMDPI AGProcesses2227-97172021-12-019141410.3390/pr9010014Study on the Extraction Technology of <i>Candida antarctica</i> Lipase B by Foam SeparationWenyao Shao0Ying Lin1Yinghua Lu2Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, ChinaDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, ChinaDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China<i>Candida antarctica</i> Lipase B (CALB) has a wide range of applications in many fields. In this study, <i>Pichia pastoris</i> was used to express CALB for fermentation tank culture. Sodium dodecyl sulfate (SDS) was used as a surfactant, and foam separation technology was used to explore the best experimental conditions for the harvest of CALB. The results showed that the optimal technological conditions for the foam separation and recovery of CALB were as follows: liquid volume was 150 mL, separating gas velocity was 600 mL/min, pH value was 7, and surfactant SDS concentration was 0.5 mg/mL. Under these conditions, the enrichment ratio of CALB was 0.95, and recovery rate R was 80.32%, respectively, indicating that the foam separation technology is feasible to extract lipase B.https://www.mdpi.com/2227-9717/9/1/14foam separationfermentationlipasesurfactantenrichment ratiorecovery rate |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wenyao Shao Ying Lin Yinghua Lu |
spellingShingle |
Wenyao Shao Ying Lin Yinghua Lu Study on the Extraction Technology of <i>Candida antarctica</i> Lipase B by Foam Separation Processes foam separation fermentation lipase surfactant enrichment ratio recovery rate |
author_facet |
Wenyao Shao Ying Lin Yinghua Lu |
author_sort |
Wenyao Shao |
title |
Study on the Extraction Technology of <i>Candida antarctica</i> Lipase B by Foam Separation |
title_short |
Study on the Extraction Technology of <i>Candida antarctica</i> Lipase B by Foam Separation |
title_full |
Study on the Extraction Technology of <i>Candida antarctica</i> Lipase B by Foam Separation |
title_fullStr |
Study on the Extraction Technology of <i>Candida antarctica</i> Lipase B by Foam Separation |
title_full_unstemmed |
Study on the Extraction Technology of <i>Candida antarctica</i> Lipase B by Foam Separation |
title_sort |
study on the extraction technology of <i>candida antarctica</i> lipase b by foam separation |
publisher |
MDPI AG |
series |
Processes |
issn |
2227-9717 |
publishDate |
2021-12-01 |
description |
<i>Candida antarctica</i> Lipase B (CALB) has a wide range of applications in many fields. In this study, <i>Pichia pastoris</i> was used to express CALB for fermentation tank culture. Sodium dodecyl sulfate (SDS) was used as a surfactant, and foam separation technology was used to explore the best experimental conditions for the harvest of CALB. The results showed that the optimal technological conditions for the foam separation and recovery of CALB were as follows: liquid volume was 150 mL, separating gas velocity was 600 mL/min, pH value was 7, and surfactant SDS concentration was 0.5 mg/mL. Under these conditions, the enrichment ratio of CALB was 0.95, and recovery rate R was 80.32%, respectively, indicating that the foam separation technology is feasible to extract lipase B. |
topic |
foam separation fermentation lipase surfactant enrichment ratio recovery rate |
url |
https://www.mdpi.com/2227-9717/9/1/14 |
work_keys_str_mv |
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