Glossogyne Tenuifolia Enhances Posttranslational S-Nitrosylation of Proteins in Vascular Endothelial Cells
Glossogyne tenuifolia (GT) is a traditional Chinese herb that possesses strong antioxidant activity and protects against endothelial cell (EC) injury by inhibition of free reactive oxygen species (ROS). The aim of this study was to elucidate the mechanisms by which GT prevents endothelial injury usi...
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doaj-f46b702abbc149f6bdb975cceaeb8a3d2020-11-25T02:29:56ZengNational Taiwan UniversityTaiwania0372-333X0372-333X2011-06-015629710410.6165/tai.2011.56(2).97Glossogyne Tenuifolia Enhances Posttranslational S-Nitrosylation of Proteins in Vascular Endothelial CellsChao-Ping Wang0Jer-Yiing Houng1Hsia-Fen Hsu2Han-Jung Chen3Bin Huang4Wei-Chin Hung5Teng-Hung Yu6Cheng-An Chiu7Li-Fen Lu8Chia-Chang Hsu9Division of Cardiology, Department of Internal Medicine, E-Da Hospital, Kaohsiung 82445, Taiwan.Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 82445, Taiwan.Department of Medical Nutrition, I-Shou University, Kaohsiung 82445, Taiwan.Division of Neurology, Department of Surgery, E-Da Hospital, Kaohsiung 82445, Taiwan.Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, 80708 Kaohsiung, Taiwan.Division of Cardiology, Department of Internal Medicine, E-Da Hospital, Kaohsiung 82445, Taiwan.Division of Cardiology, Department of Internal Medicine, E-Da Hospital, Kaohsiung 82445, Taiwan.Division of Cardiology, Department of Internal Medicine, E-Da Hospital, Kaohsiung 82445, Taiwan.Division of Cardiac Surgery, Department of Surgery, E-Da Hospital, Kaohsiung 82445, Taiwan.Division of Gastroenterology Hepatology, Department of Internal Medicine, E-Da Hospital, Kaohsiung 82445, Taiwan.Glossogyne tenuifolia (GT) is a traditional Chinese herb that possesses strong antioxidant activity and protects against endothelial cell (EC) injury by inhibition of free reactive oxygen species (ROS). The aim of this study was to elucidate the mechanisms by which GT prevents endothelial injury using a proteomics approach. We used a sensitive method to analyze the S- nitrosoproteins utilizing a modified biotin-switch method in order to detect the possible effects of GT on protein posttranslational modification. After treatment of vascular ECs with GT, two proteins HspA9 (IS1), beta-actin (IS2) were observed to have increased posttranslational S-nitrosylation, whereas seven proteins, vimentin (DS2, DS3 and DS5), tropomyosin 3, 4 (DS6 and DS7) and oxidative phosphorylation protein such as ATP synthase, F1 complex (DS1) and 80K-H protein (DS4), were found to have decreased posttranslational S-nitrosylation. Due to S-nitrosylation of HspA9 causing the reduction of intracellular ROS and S-nitrosylation of ATP synthase interfering with ATP production and ROS formation, our study may indicate a novel mechanism in which GT protects EC injury by the inhibition of oxidative reaction.http://tai2.ntu.edu.tw/taiwania/abstract.php?type=abstract&id=1064Antioxidationfree reactive oxygen speciesGlossogyne tenuifoliaposttranslational S-nitrosylation of proteinsvascular endothelial cells. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chao-Ping Wang Jer-Yiing Houng Hsia-Fen Hsu Han-Jung Chen Bin Huang Wei-Chin Hung Teng-Hung Yu Cheng-An Chiu Li-Fen Lu Chia-Chang Hsu |
spellingShingle |
Chao-Ping Wang Jer-Yiing Houng Hsia-Fen Hsu Han-Jung Chen Bin Huang Wei-Chin Hung Teng-Hung Yu Cheng-An Chiu Li-Fen Lu Chia-Chang Hsu Glossogyne Tenuifolia Enhances Posttranslational S-Nitrosylation of Proteins in Vascular Endothelial Cells Taiwania Antioxidation free reactive oxygen species Glossogyne tenuifolia posttranslational S-nitrosylation of proteins vascular endothelial cells. |
author_facet |
Chao-Ping Wang Jer-Yiing Houng Hsia-Fen Hsu Han-Jung Chen Bin Huang Wei-Chin Hung Teng-Hung Yu Cheng-An Chiu Li-Fen Lu Chia-Chang Hsu |
author_sort |
Chao-Ping Wang |
title |
Glossogyne Tenuifolia Enhances Posttranslational S-Nitrosylation of Proteins in Vascular Endothelial Cells |
title_short |
Glossogyne Tenuifolia Enhances Posttranslational S-Nitrosylation of Proteins in Vascular Endothelial Cells |
title_full |
Glossogyne Tenuifolia Enhances Posttranslational S-Nitrosylation of Proteins in Vascular Endothelial Cells |
title_fullStr |
Glossogyne Tenuifolia Enhances Posttranslational S-Nitrosylation of Proteins in Vascular Endothelial Cells |
title_full_unstemmed |
Glossogyne Tenuifolia Enhances Posttranslational S-Nitrosylation of Proteins in Vascular Endothelial Cells |
title_sort |
glossogyne tenuifolia enhances posttranslational s-nitrosylation of proteins in vascular endothelial cells |
publisher |
National Taiwan University |
series |
Taiwania |
issn |
0372-333X 0372-333X |
publishDate |
2011-06-01 |
description |
Glossogyne tenuifolia (GT) is a traditional Chinese herb that possesses strong antioxidant activity and protects against endothelial cell (EC) injury by inhibition of free reactive oxygen species (ROS). The aim of this study was to elucidate the mechanisms by which GT prevents endothelial injury using a proteomics approach. We used a sensitive method to analyze the S- nitrosoproteins utilizing a modified biotin-switch method in order to detect the possible effects of GT on protein posttranslational modification. After treatment of vascular ECs with GT, two proteins HspA9 (IS1), beta-actin (IS2) were observed to have increased posttranslational S-nitrosylation, whereas seven proteins, vimentin (DS2, DS3 and DS5), tropomyosin 3, 4 (DS6 and DS7) and oxidative phosphorylation protein such as ATP synthase, F1 complex (DS1) and 80K-H protein (DS4), were found to have decreased posttranslational S-nitrosylation. Due to S-nitrosylation of HspA9 causing the reduction of intracellular ROS and S-nitrosylation of ATP synthase interfering with ATP production and ROS formation, our study may indicate a novel mechanism in which GT protects EC injury by the inhibition of oxidative reaction. |
topic |
Antioxidation free reactive oxygen species Glossogyne tenuifolia posttranslational S-nitrosylation of proteins vascular endothelial cells. |
url |
http://tai2.ntu.edu.tw/taiwania/abstract.php?type=abstract&id=1064 |
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