Glossogyne Tenuifolia Enhances Posttranslational S-Nitrosylation of Proteins in Vascular Endothelial Cells

• Main Authors: Chao-Ping Wang, Jer-Yiing Houng, Hsia-Fen Hsu, Han-Jung Chen, Bin Huang, Wei-Chin Hung, Teng-Hung Yu, Cheng-An Chiu, Li-Fen Lu, Chia-Chang Hsu
• Format: Article
• Language: English
• Published: National Taiwan University 2011-06-01
• Series: Taiwania
• Subjects: Antioxidation; free reactive oxygen species; Glossogyne tenuifolia; posttranslational S-nitrosylation of proteins; vascular endothelial cells.
• Online Access: http://tai2.ntu.edu.tw/taiwania/abstract.php?type=abstract&id=1064

Glossogyne tenuifolia (GT) is a traditional Chinese herb that possesses strong antioxidant activity and protects against endothelial cell (EC) injury by inhibition of free reactive oxygen species (ROS). The aim of this study was to elucidate the mechanisms by which GT prevents endothelial injury using a proteomics approach. We used a sensitive method to analyze the S- nitrosoproteins utilizing a modified biotin-switch method in order to detect the possible effects of GT on protein posttranslational modification. After treatment of vascular ECs with GT, two proteins HspA9 (IS1), beta-actin (IS2) were observed to have increased posttranslational S-nitrosylation, whereas seven proteins, vimentin (DS2, DS3 and DS5), tropomyosin 3, 4 (DS6 and DS7) and oxidative phosphorylation protein such as ATP synthase, F1 complex (DS1) and 80K-H protein (DS4), were found to have decreased posttranslational S-nitrosylation. Due to S-nitrosylation of HspA9 causing the reduction of intracellular ROS and S-nitrosylation of ATP synthase interfering with ATP production and ROS formation, our study may indicate a novel mechanism in which GT protects EC injury by the inhibition of oxidative reaction.