Tissue transglutaminase in marmoset experimental multiple sclerosis: discrepancy between white and grey matter.
Infiltration of leukocytes is a major pathological event in white matter lesion formation in the brain of multiple sclerosis (MS) patients. In grey matter lesions, less infiltration of these cells occur, but microglial activation is present. Thus far, the interaction of β-integrins with extracellula...
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doaj-f4884f653e4e41fbaaae4fdc706b01e82020-11-25T02:50:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e10057410.1371/journal.pone.0100574Tissue transglutaminase in marmoset experimental multiple sclerosis: discrepancy between white and grey matter.Nathaly Espitia PinzonEsther StrooBert A 't HartJohn G J M BolBenjamin DrukarchJan BauerAnne-Marie van DamInfiltration of leukocytes is a major pathological event in white matter lesion formation in the brain of multiple sclerosis (MS) patients. In grey matter lesions, less infiltration of these cells occur, but microglial activation is present. Thus far, the interaction of β-integrins with extracellular matrix proteins, e.g. fibronectin, is considered to be of importance for the influx of immune cells. Recent in vitro studies indicate a possible role for the enzyme tissue Transglutaminase (TG2) in mediating cell adhesion and migration. In the present study we questioned whether TG2 is present in white and grey matter lesions observed in the marmoset model for MS. To this end, immunohistochemical studies were performed. We observed that TG2, expressed by infiltrating monocytes in white matter lesions co-expressed β1-integrin and is located in close apposition to deposited fibronectin. These data suggest an important role for TG2 in the adhesion and migration of infiltrating monocytes during white matter lesion formation. Moreover, in grey matter lesions, TG2 is mainly present in microglial cells together with some β1-integrin, whereas fibronectin is absent in these lesions. These data imply an alternative role for microglial-derived TG2 in grey matter lesions, e.g. cell proliferation. Further research should clarify the functional role of TG2 in monocytes or microglial cells in MS lesion formation.http://europepmc.org/articles/PMC4069090?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nathaly Espitia Pinzon Esther Stroo Bert A 't Hart John G J M Bol Benjamin Drukarch Jan Bauer Anne-Marie van Dam |
spellingShingle |
Nathaly Espitia Pinzon Esther Stroo Bert A 't Hart John G J M Bol Benjamin Drukarch Jan Bauer Anne-Marie van Dam Tissue transglutaminase in marmoset experimental multiple sclerosis: discrepancy between white and grey matter. PLoS ONE |
author_facet |
Nathaly Espitia Pinzon Esther Stroo Bert A 't Hart John G J M Bol Benjamin Drukarch Jan Bauer Anne-Marie van Dam |
author_sort |
Nathaly Espitia Pinzon |
title |
Tissue transglutaminase in marmoset experimental multiple sclerosis: discrepancy between white and grey matter. |
title_short |
Tissue transglutaminase in marmoset experimental multiple sclerosis: discrepancy between white and grey matter. |
title_full |
Tissue transglutaminase in marmoset experimental multiple sclerosis: discrepancy between white and grey matter. |
title_fullStr |
Tissue transglutaminase in marmoset experimental multiple sclerosis: discrepancy between white and grey matter. |
title_full_unstemmed |
Tissue transglutaminase in marmoset experimental multiple sclerosis: discrepancy between white and grey matter. |
title_sort |
tissue transglutaminase in marmoset experimental multiple sclerosis: discrepancy between white and grey matter. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Infiltration of leukocytes is a major pathological event in white matter lesion formation in the brain of multiple sclerosis (MS) patients. In grey matter lesions, less infiltration of these cells occur, but microglial activation is present. Thus far, the interaction of β-integrins with extracellular matrix proteins, e.g. fibronectin, is considered to be of importance for the influx of immune cells. Recent in vitro studies indicate a possible role for the enzyme tissue Transglutaminase (TG2) in mediating cell adhesion and migration. In the present study we questioned whether TG2 is present in white and grey matter lesions observed in the marmoset model for MS. To this end, immunohistochemical studies were performed. We observed that TG2, expressed by infiltrating monocytes in white matter lesions co-expressed β1-integrin and is located in close apposition to deposited fibronectin. These data suggest an important role for TG2 in the adhesion and migration of infiltrating monocytes during white matter lesion formation. Moreover, in grey matter lesions, TG2 is mainly present in microglial cells together with some β1-integrin, whereas fibronectin is absent in these lesions. These data imply an alternative role for microglial-derived TG2 in grey matter lesions, e.g. cell proliferation. Further research should clarify the functional role of TG2 in monocytes or microglial cells in MS lesion formation. |
url |
http://europepmc.org/articles/PMC4069090?pdf=render |
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