Assessment of antibacterial, anti-inflammatory, and cytotoxic effects of different extracts of Gynura procumbens leaf
ABSTRACT: Background: In Southeast Asia, Gynura procumbens has been utilized as a traditional medicinal herb for many diseases. The nontoxic effects of the leaves of G procumbens can be consumed safely for the treatment of many diseases, especially diabetes mellitus. Objectives: This study aimed to...
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doaj-f48fb8425aa7456cb2daae4c8a65aaeb2021-07-25T04:40:00ZengElsevierCurrent Therapeutic Research0011-393X2021-01-0195100636Assessment of antibacterial, anti-inflammatory, and cytotoxic effects of different extracts of Gynura procumbens leafMd. Ziaul Amin, Ph.D.0Mitu Afrin, MS1Nigar Sultana Meghla, MS2Md. Ashaduzzaman Nur, MS3Md. Mashiar Rahman, Ph.D.4M. Jashim Uddin, MS5Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore, Bangladesh; Address correspondence to: Md. Ziaul Amin, PhD, Department of Genetic Engineering and Biotechnology, Jessore University of Science and Technology, Jessore-7408, Bangladesh.Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore, BangladeshDepartment of Microbiology, Jashore University of Science and Technology, Jashore, BangladeshDepartment of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore, BangladeshDepartment of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore, BangladeshDepartment of Pharmacy, Jashore University of Science and Technology, Jashore, BangladeshABSTRACT: Background: In Southeast Asia, Gynura procumbens has been utilized as a traditional medicinal herb for many diseases. The nontoxic effects of the leaves of G procumbens can be consumed safely for the treatment of many diseases, especially diabetes mellitus. Objectives: This study aimed to assess the antibacterial, anti-inflammatory, and cytotoxic effects of G procumbens leaves through different extracts. Methods: Cold extraction was employed for G procumbens leaves. Disc diffusion, bovine serum albumin denaturation, brine shrimp lethality assays, and microscopic examination of tissues (ie, liver, kidney, and heart) were performed to measure antibacterial, anti-inflammatory, and cytotoxic activities, and histopathologic analysis, respectively. Results: The distinct concentrations of aqueous, ethanol, and n-hexane extracts showed prominent antibacterial activity against four pathogenic bacterial strains (Chromobacterium sp, Staphylococcus aureus, Enterococcus faecium, and Escherichia coli 0157:H7:LT). The aqueous extract of G procumbens at a concentration of 200 µg/mL showed potential antibacterial activities against S aureus and E faecium, with mean (SD) zones of inhibition of 15 (1.0) mm and 10 (0.55) mm, respectively. At a concentration of 40 µg/mL, the aqueous extract of G procumbens exhibited significant (P < 0.01) anti-inflammatory activity compared with that of the other 2 extracts in the bovine serum albumin denaturation assay. The n-hexane extract showed moderate cytotoxic activity compared with that of vincristine sulfate. The median lethal concentration values of the aqueous, ethanol, and n-hexane extracts were 427.66, 218.56, and 114.51 μg/mL, respectively. The aqueous extract exhibited lower cytotoxicity than that exhibited by the ethanol and n-hexane extracts. The histopathologic analysis results showed that the aqueous extract of G procumbens had no harmful effects on the different organs of the experimental mice. Conclusions: The aqueous extract of G procumbens could be a potential source for treating various infectious and chronic diseases. (Curr Ther Res Clin Exp. 2021; 82:XXX–XXX)http://www.sciencedirect.com/science/article/pii/S0011393X2100014XAntibacterial activityanti-inflammatory activitycytotoxicityGynura procumbenshistopathologylethality bioassay |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Md. Ziaul Amin, Ph.D. Mitu Afrin, MS Nigar Sultana Meghla, MS Md. Ashaduzzaman Nur, MS Md. Mashiar Rahman, Ph.D. M. Jashim Uddin, MS |
spellingShingle |
Md. Ziaul Amin, Ph.D. Mitu Afrin, MS Nigar Sultana Meghla, MS Md. Ashaduzzaman Nur, MS Md. Mashiar Rahman, Ph.D. M. Jashim Uddin, MS Assessment of antibacterial, anti-inflammatory, and cytotoxic effects of different extracts of Gynura procumbens leaf Current Therapeutic Research Antibacterial activity anti-inflammatory activity cytotoxicity Gynura procumbens histopathology lethality bioassay |
author_facet |
Md. Ziaul Amin, Ph.D. Mitu Afrin, MS Nigar Sultana Meghla, MS Md. Ashaduzzaman Nur, MS Md. Mashiar Rahman, Ph.D. M. Jashim Uddin, MS |
author_sort |
Md. Ziaul Amin, Ph.D. |
title |
Assessment of antibacterial, anti-inflammatory, and cytotoxic effects of different extracts of Gynura procumbens leaf |
title_short |
Assessment of antibacterial, anti-inflammatory, and cytotoxic effects of different extracts of Gynura procumbens leaf |
title_full |
Assessment of antibacterial, anti-inflammatory, and cytotoxic effects of different extracts of Gynura procumbens leaf |
title_fullStr |
Assessment of antibacterial, anti-inflammatory, and cytotoxic effects of different extracts of Gynura procumbens leaf |
title_full_unstemmed |
Assessment of antibacterial, anti-inflammatory, and cytotoxic effects of different extracts of Gynura procumbens leaf |
title_sort |
assessment of antibacterial, anti-inflammatory, and cytotoxic effects of different extracts of gynura procumbens leaf |
publisher |
Elsevier |
series |
Current Therapeutic Research |
issn |
0011-393X |
publishDate |
2021-01-01 |
description |
ABSTRACT: Background: In Southeast Asia, Gynura procumbens has been utilized as a traditional medicinal herb for many diseases. The nontoxic effects of the leaves of G procumbens can be consumed safely for the treatment of many diseases, especially diabetes mellitus. Objectives: This study aimed to assess the antibacterial, anti-inflammatory, and cytotoxic effects of G procumbens leaves through different extracts. Methods: Cold extraction was employed for G procumbens leaves. Disc diffusion, bovine serum albumin denaturation, brine shrimp lethality assays, and microscopic examination of tissues (ie, liver, kidney, and heart) were performed to measure antibacterial, anti-inflammatory, and cytotoxic activities, and histopathologic analysis, respectively. Results: The distinct concentrations of aqueous, ethanol, and n-hexane extracts showed prominent antibacterial activity against four pathogenic bacterial strains (Chromobacterium sp, Staphylococcus aureus, Enterococcus faecium, and Escherichia coli 0157:H7:LT). The aqueous extract of G procumbens at a concentration of 200 µg/mL showed potential antibacterial activities against S aureus and E faecium, with mean (SD) zones of inhibition of 15 (1.0) mm and 10 (0.55) mm, respectively. At a concentration of 40 µg/mL, the aqueous extract of G procumbens exhibited significant (P < 0.01) anti-inflammatory activity compared with that of the other 2 extracts in the bovine serum albumin denaturation assay. The n-hexane extract showed moderate cytotoxic activity compared with that of vincristine sulfate. The median lethal concentration values of the aqueous, ethanol, and n-hexane extracts were 427.66, 218.56, and 114.51 μg/mL, respectively. The aqueous extract exhibited lower cytotoxicity than that exhibited by the ethanol and n-hexane extracts. The histopathologic analysis results showed that the aqueous extract of G procumbens had no harmful effects on the different organs of the experimental mice. Conclusions: The aqueous extract of G procumbens could be a potential source for treating various infectious and chronic diseases. (Curr Ther Res Clin Exp. 2021; 82:XXX–XXX) |
topic |
Antibacterial activity anti-inflammatory activity cytotoxicity Gynura procumbens histopathology lethality bioassay |
url |
http://www.sciencedirect.com/science/article/pii/S0011393X2100014X |
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