Survival of tubercle bacilli in heat-fixed and stained sputum smears

We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None o...

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Bibliographic Details
Main Authors: Celso Luíz Cardoso, Luiz Roberto Bigão Giacomelli, César Helbel, Janete Jordão Sant'Ana, Fátima Moreira Martins, Angela Maria Werneck Barreto
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2001-02-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200024

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