Ultrasonic assisted extraction to obtain bioactive, antioxidant and antimicrobial compounds from marcela
ABSTRACT: The aim of this study was to evaluate the effect of extraction conditions on bioactive compounds, as well as on antioxidant activity, and the antimicrobial activity of the extracts with the highest antioxidant characteristics. The extracts were obtained by conventional method and ultrasou...
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Universidade Federal de Santa Maria
2018-06-01
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doaj-f4b1d45622e041e493800f3175490f032020-11-24T21:08:01ZengUniversidade Federal de Santa MariaCiência Rural1678-45962018-06-0148610.1590/0103-8478cr20170772S0103-84782018000600201Ultrasonic assisted extraction to obtain bioactive, antioxidant and antimicrobial compounds from marcelaCaroline Pagnossim BoeiraNatiéli PiovesanMarcela Bromberger SoquettaDéborah Cristina Barcelos FloresBruna Nichelle LucasJuliano Smanioto BarinClaudia Severo da RosaNelcindo Nascimento TerraABSTRACT: The aim of this study was to evaluate the effect of extraction conditions on bioactive compounds, as well as on antioxidant activity, and the antimicrobial activity of the extracts with the highest antioxidant characteristics. The extracts were obtained by conventional method and ultrasound-assisted extraction at various temperatures (20°C, 40°C and 60°C). Total phenolics, total flavonoids, antimicrobial activity and antioxidant activity were quantified by the methods of DPPH, FRAP, and ORAC, respectively. The conventional extraction method and ultrasound method influenced the phenolic content at all the tested temperatures. Flavonoids were not influenced by extraction methods. The antioxidant activity (DPPH) was highest in the ultrasonic method at temperatures of 40°C and 60°C; however, in the case of the FRAP method the best results were for the conventional extraction method. The conventional and ultrasonic methods did not influence the IC50 at temperatures of 20°C and 40°C, but using ORAC the antioxidant activity was influenced by the methods at all temperatures. The extract obtained at 60°C by the ultrasound method had high antimicrobial action in relation to the strains of Salmonella sp., Escherichia coli, and Staphylococcus aureus. Extraction ultrasonic-assisted can be considered adequate to obtain extracts of marcela, which are rich in bioactive compounds with high antioxidant activity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600201&lng=en&tlng=enultrassomcompostos bioativosantioxidantes naturaisAchyrocline satureioides |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Caroline Pagnossim Boeira Natiéli Piovesan Marcela Bromberger Soquetta Déborah Cristina Barcelos Flores Bruna Nichelle Lucas Juliano Smanioto Barin Claudia Severo da Rosa Nelcindo Nascimento Terra |
spellingShingle |
Caroline Pagnossim Boeira Natiéli Piovesan Marcela Bromberger Soquetta Déborah Cristina Barcelos Flores Bruna Nichelle Lucas Juliano Smanioto Barin Claudia Severo da Rosa Nelcindo Nascimento Terra Ultrasonic assisted extraction to obtain bioactive, antioxidant and antimicrobial compounds from marcela Ciência Rural ultrassom compostos bioativos antioxidantes naturais Achyrocline satureioides |
author_facet |
Caroline Pagnossim Boeira Natiéli Piovesan Marcela Bromberger Soquetta Déborah Cristina Barcelos Flores Bruna Nichelle Lucas Juliano Smanioto Barin Claudia Severo da Rosa Nelcindo Nascimento Terra |
author_sort |
Caroline Pagnossim Boeira |
title |
Ultrasonic assisted extraction to obtain bioactive, antioxidant and antimicrobial compounds from marcela |
title_short |
Ultrasonic assisted extraction to obtain bioactive, antioxidant and antimicrobial compounds from marcela |
title_full |
Ultrasonic assisted extraction to obtain bioactive, antioxidant and antimicrobial compounds from marcela |
title_fullStr |
Ultrasonic assisted extraction to obtain bioactive, antioxidant and antimicrobial compounds from marcela |
title_full_unstemmed |
Ultrasonic assisted extraction to obtain bioactive, antioxidant and antimicrobial compounds from marcela |
title_sort |
ultrasonic assisted extraction to obtain bioactive, antioxidant and antimicrobial compounds from marcela |
publisher |
Universidade Federal de Santa Maria |
series |
Ciência Rural |
issn |
1678-4596 |
publishDate |
2018-06-01 |
description |
ABSTRACT: The aim of this study was to evaluate the effect of extraction conditions on bioactive compounds, as well as on antioxidant activity, and the antimicrobial activity of the extracts with the highest antioxidant characteristics. The extracts were obtained by conventional method and ultrasound-assisted extraction at various temperatures (20°C, 40°C and 60°C). Total phenolics, total flavonoids, antimicrobial activity and antioxidant activity were quantified by the methods of DPPH, FRAP, and ORAC, respectively. The conventional extraction method and ultrasound method influenced the phenolic content at all the tested temperatures. Flavonoids were not influenced by extraction methods. The antioxidant activity (DPPH) was highest in the ultrasonic method at temperatures of 40°C and 60°C; however, in the case of the FRAP method the best results were for the conventional extraction method. The conventional and ultrasonic methods did not influence the IC50 at temperatures of 20°C and 40°C, but using ORAC the antioxidant activity was influenced by the methods at all temperatures. The extract obtained at 60°C by the ultrasound method had high antimicrobial action in relation to the strains of Salmonella sp., Escherichia coli, and Staphylococcus aureus. Extraction ultrasonic-assisted can be considered adequate to obtain extracts of marcela, which are rich in bioactive compounds with high antioxidant activity. |
topic |
ultrassom compostos bioativos antioxidantes naturais Achyrocline satureioides |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600201&lng=en&tlng=en |
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