The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertility
Summary By a conserved cellular differentiation process, spermatogenesis leads to formation of haploid sperm for successful reproduction. In Drosophila and in mammals, post-meiotic spermatid differentiation depends on several translationally repressed and stored mRNAs that are often expressed exclus...
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doaj-f4b31b12655a46179d793cd505c871f82021-06-02T18:47:13ZengThe Company of BiologistsBiology Open2046-63902012-05-011659760610.1242/bio.2012125520121255The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertilityKatja LeserStephan Awe0Bridlin Barckmann1Renate Renkawitz-PohlChristina Rathke Present address: Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg, Emil-Mannkopff-Strasse 2, 35037 Marburg, Germany Present address: Institut de Génétique Humaine, CNRS UPR 1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France Summary By a conserved cellular differentiation process, spermatogenesis leads to formation of haploid sperm for successful reproduction. In Drosophila and in mammals, post-meiotic spermatid differentiation depends on several translationally repressed and stored mRNAs that are often expressed exclusively in the testis through a cell type specific transcriptional program. In Drosophila, the mRNAs of proteins required for post-meiotic chromatin reorganisation, like ProtB and Mst77F, are transcribed in meiotic spermatocytes and subjected to translational repression for days. Transcription of many of these translationally repressed mRNAs depends on testis-specific homologs of TATA box binding protein-associated factors (tTAFs). Here, we identified the testis-specific bromodomain protein, tBRD-1, that is only expressed in primary spermatocytes. Bromodomain proteins are able to recognise and bind acetylated histones and non-histone proteins. We generated tbrd-1 mutant flies and observed that function of tBRD-1 is required for male fertility. tBRD-1 partially colocalised with tTAFs, TAF1 and Polycomb to a Fibrillarin-deficient region within the spermatocyte nucleolus. The nucleolar localisation of tBRD-1 depended on tTAF function but not the other way round. Further, we could show that ectopically expressed tBRD-1-eGFP is able to bind to the interbands of polytene chromosomes. By inhibitor treatment of cultured testis we observed that sub-cellular localisation of tBRD-1 may depend on the acetylation status of primary spermatocytes.http://bio.biologists.org/content/1/6/597DrosophilaAcetylationBromodomain proteinsPolycombSpermatogenesistTAF |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katja Leser Stephan Awe Bridlin Barckmann Renate Renkawitz-Pohl Christina Rathke |
spellingShingle |
Katja Leser Stephan Awe Bridlin Barckmann Renate Renkawitz-Pohl Christina Rathke The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertility Biology Open Drosophila Acetylation Bromodomain proteins Polycomb Spermatogenesis tTAF |
author_facet |
Katja Leser Stephan Awe Bridlin Barckmann Renate Renkawitz-Pohl Christina Rathke |
author_sort |
Katja Leser |
title |
The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertility |
title_short |
The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertility |
title_full |
The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertility |
title_fullStr |
The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertility |
title_full_unstemmed |
The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertility |
title_sort |
bromodomain-containing protein tbrd-1 is specifically expressed in spermatocytes and is essential for male fertility |
publisher |
The Company of Biologists |
series |
Biology Open |
issn |
2046-6390 |
publishDate |
2012-05-01 |
description |
Summary
By a conserved cellular differentiation process, spermatogenesis leads to formation of haploid sperm for successful reproduction. In Drosophila and in mammals, post-meiotic spermatid differentiation depends on several translationally repressed and stored mRNAs that are often expressed exclusively in the testis through a cell type specific transcriptional program. In Drosophila, the mRNAs of proteins required for post-meiotic chromatin reorganisation, like ProtB and Mst77F, are transcribed in meiotic spermatocytes and subjected to translational repression for days. Transcription of many of these translationally repressed mRNAs depends on testis-specific homologs of TATA box binding protein-associated factors (tTAFs). Here, we identified the testis-specific bromodomain protein, tBRD-1, that is only expressed in primary spermatocytes. Bromodomain proteins are able to recognise and bind acetylated histones and non-histone proteins. We generated tbrd-1 mutant flies and observed that function of tBRD-1 is required for male fertility. tBRD-1 partially colocalised with tTAFs, TAF1 and Polycomb to a Fibrillarin-deficient region within the spermatocyte nucleolus. The nucleolar localisation of tBRD-1 depended on tTAF function but not the other way round. Further, we could show that ectopically expressed tBRD-1-eGFP is able to bind to the interbands of polytene chromosomes. By inhibitor treatment of cultured testis we observed that sub-cellular localisation of tBRD-1 may depend on the acetylation status of primary spermatocytes. |
topic |
Drosophila Acetylation Bromodomain proteins Polycomb Spermatogenesis tTAF |
url |
http://bio.biologists.org/content/1/6/597 |
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