Optimization of TBAB-Assisted Lipase-catalyzed Synthesis of L-Ascorbyl Myristate
The synthesis of l-ascorbyl myristate through esterification of l-ascorbic acid and myristic acid catalyzed by lipase was studied. The effect of different parameters including lipase, molecular sieve 4 Å, organic solvent, and tetrabutyl ammonium bromide (TBAB) was evaluated. The results showed exce...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Croatian Society of Chemical Engineers
2018-07-01
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| Series: | Chemical and Biochemical Engineering Quarterly |
| Subjects: | |
| Online Access: | http://silverstripe.fkit.hr/cabeq/assets/Uploads/09-2-18.pdf |
| Summary: | The synthesis of l-ascorbyl myristate through esterification of l-ascorbic acid and myristic acid catalyzed by lipase was studied. The effect of different parameters including lipase, molecular sieve 4 Å, organic solvent, and tetrabutyl ammonium bromide (TBAB)
was evaluated. The results showed excellent yield in the presence of Novozyme435 as catalyst, 10 % (w/w of l-ascorbic acid) of 4 Å molecular sieve and 10 % (w/w of l-ascorbic acid) of TBAB as additives in 1,4-dioxane. Furthermore, response surface methodology (RSM) and 5-level-4-factor central composite design (CCD) were employed to evaluate the effects of parameters on the yield of l-ascorbyl myristate. According to the analysis result of ridge max, the optimal synthesis conditions were predicted, as follows: reaction time 27.1 h, temperature 54.1 °C, enzyme amount 25.9 % (% w/w of l-ascorbic acid), substrate molar ratio 5, and the optimal actual yield was 85.0 %. |
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| ISSN: | 0352-9568 1846-5153 |