Simplified fluorometric method for the determination of plasma glycerol
A simplified method for determining plasma glycerol is described. This assay utilizes the fluorometric measurement of the reduced adenine dinucleotide, NADH2 which is formed when glycerol is oxidized by glycerol dehydrogenase. Only three pipettings are necessary for each reaction tube, and a large n...
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1970-11-01
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520429463 |
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doaj-f4c018f0f9654de6918e321db042b71f2021-04-24T05:54:47ZengElsevierJournal of Lipid Research0022-22751970-11-01116609612Simplified fluorometric method for the determination of plasma glycerolMayer B. Davidson0Robert Karjala1Department of Medicine, University of California at Los Angeles School of Medicine, and Wadsworth Veterans Administration Hospital, Los Angeles, California 90024Department of Medicine, University of California at Los Angeles School of Medicine, and Wadsworth Veterans Administration Hospital, Los Angeles, California 90024A simplified method for determining plasma glycerol is described. This assay utilizes the fluorometric measurement of the reduced adenine dinucleotide, NADH2 which is formed when glycerol is oxidized by glycerol dehydrogenase. Only three pipettings are necessary for each reaction tube, and a large number of samples can be included in each assay.http://www.sciencedirect.com/science/article/pii/S0022227520429463glycerolglycerol dehydrogenaseNADH2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mayer B. Davidson Robert Karjala |
spellingShingle |
Mayer B. Davidson Robert Karjala Simplified fluorometric method for the determination of plasma glycerol Journal of Lipid Research glycerol glycerol dehydrogenase NADH2 |
author_facet |
Mayer B. Davidson Robert Karjala |
author_sort |
Mayer B. Davidson |
title |
Simplified fluorometric method for the determination of plasma glycerol |
title_short |
Simplified fluorometric method for the determination of plasma glycerol |
title_full |
Simplified fluorometric method for the determination of plasma glycerol |
title_fullStr |
Simplified fluorometric method for the determination of plasma glycerol |
title_full_unstemmed |
Simplified fluorometric method for the determination of plasma glycerol |
title_sort |
simplified fluorometric method for the determination of plasma glycerol |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1970-11-01 |
description |
A simplified method for determining plasma glycerol is described. This assay utilizes the fluorometric measurement of the reduced adenine dinucleotide, NADH2 which is formed when glycerol is oxidized by glycerol dehydrogenase. Only three pipettings are necessary for each reaction tube, and a large number of samples can be included in each assay. |
topic |
glycerol glycerol dehydrogenase NADH2 |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520429463 |
work_keys_str_mv |
AT mayerbdavidson simplifiedfluorometricmethodforthedeterminationofplasmaglycerol AT robertkarjala simplifiedfluorometricmethodforthedeterminationofplasmaglycerol |
_version_ |
1721511373657079808 |