Simplified fluorometric method for the determination of plasma glycerol

A simplified method for determining plasma glycerol is described. This assay utilizes the fluorometric measurement of the reduced adenine dinucleotide, NADH2 which is formed when glycerol is oxidized by glycerol dehydrogenase. Only three pipettings are necessary for each reaction tube, and a large n...

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Bibliographic Details
Main Authors: Mayer B. Davidson, Robert Karjala
Format: Article
Language:English
Published: Elsevier 1970-11-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520429463
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spelling doaj-f4c018f0f9654de6918e321db042b71f2021-04-24T05:54:47ZengElsevierJournal of Lipid Research0022-22751970-11-01116609612Simplified fluorometric method for the determination of plasma glycerolMayer B. Davidson0Robert Karjala1Department of Medicine, University of California at Los Angeles School of Medicine, and Wadsworth Veterans Administration Hospital, Los Angeles, California 90024Department of Medicine, University of California at Los Angeles School of Medicine, and Wadsworth Veterans Administration Hospital, Los Angeles, California 90024A simplified method for determining plasma glycerol is described. This assay utilizes the fluorometric measurement of the reduced adenine dinucleotide, NADH2 which is formed when glycerol is oxidized by glycerol dehydrogenase. Only three pipettings are necessary for each reaction tube, and a large number of samples can be included in each assay.http://www.sciencedirect.com/science/article/pii/S0022227520429463glycerolglycerol dehydrogenaseNADH2
collection DOAJ
language English
format Article
sources DOAJ
author Mayer B. Davidson
Robert Karjala
spellingShingle Mayer B. Davidson
Robert Karjala
Simplified fluorometric method for the determination of plasma glycerol
Journal of Lipid Research
glycerol
glycerol dehydrogenase
NADH2
author_facet Mayer B. Davidson
Robert Karjala
author_sort Mayer B. Davidson
title Simplified fluorometric method for the determination of plasma glycerol
title_short Simplified fluorometric method for the determination of plasma glycerol
title_full Simplified fluorometric method for the determination of plasma glycerol
title_fullStr Simplified fluorometric method for the determination of plasma glycerol
title_full_unstemmed Simplified fluorometric method for the determination of plasma glycerol
title_sort simplified fluorometric method for the determination of plasma glycerol
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1970-11-01
description A simplified method for determining plasma glycerol is described. This assay utilizes the fluorometric measurement of the reduced adenine dinucleotide, NADH2 which is formed when glycerol is oxidized by glycerol dehydrogenase. Only three pipettings are necessary for each reaction tube, and a large number of samples can be included in each assay.
topic glycerol
glycerol dehydrogenase
NADH2
url http://www.sciencedirect.com/science/article/pii/S0022227520429463
work_keys_str_mv AT mayerbdavidson simplifiedfluorometricmethodforthedeterminationofplasmaglycerol
AT robertkarjala simplifiedfluorometricmethodforthedeterminationofplasmaglycerol
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