RBM20-Mediated Pre-mRNA Splicing Has Muscle-Specificity and Differential Hormonal Responses between Muscles and in Muscle Cell Cultures
Pre-mRNA splicing plays an important role in muscle function and diseases. The RNA binding motif 20 (RBM20) is a splicing factor that is predominantly expressed in muscle tissues and primarily regulates pre-mRNA splicing of Ttn, encoding a giant muscle protein titin that is responsible for muscle fu...
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doaj-f4ebe107cbbd458cafc7532c3c86e5562021-03-14T00:02:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01222928292810.3390/ijms22062928RBM20-Mediated Pre-mRNA Splicing Has Muscle-Specificity and Differential Hormonal Responses between Muscles and in Muscle Cell CulturesRexiati Maimaiti0Chaoqun Zhu1Yanghai Zhang2Qiyue Ding3Wei Guo4Animal Science Department, University of Wyoming, Laramie, WY 82071, USAAnimal Science Department, University of Wyoming, Laramie, WY 82071, USAAnimal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706, USAAnimal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706, USAAnimal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706, USAPre-mRNA splicing plays an important role in muscle function and diseases. The RNA binding motif 20 (RBM20) is a splicing factor that is predominantly expressed in muscle tissues and primarily regulates pre-mRNA splicing of Ttn, encoding a giant muscle protein titin that is responsible for muscle function and diseases. RBM20-mediated Ttn splicing has been mostly studied in heart muscle, but not in skeletal muscle. In this study, we investigated splicing specificity in different muscle types in Rbm20 knockout rats and hormonal effects on RBM20-mediated splicing both in cellulo and in vivo studies. The results revealed that RBM20 is differentially expressed across muscles and RBM20-mediated splicing is muscle-type specific. In the presence of RBM20, Ttn splicing responds to hormones in a muscle-type dependent manner, while in the absence of RBM20, Ttn splicing is not affected by hormones. In differentiated and undifferentiated C2C12 cells, RBM20-mediated splicing in response to hormonal effects is mainly through genomic signaling pathway. The knowledge gained from this study may help further understand muscle-specific gene splicing in response to hormone stimuli in different muscle types.https://www.mdpi.com/1422-0067/22/6/2928RBM20pre-mRNA splicingtitinhormonesskeletal muscle |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rexiati Maimaiti Chaoqun Zhu Yanghai Zhang Qiyue Ding Wei Guo |
spellingShingle |
Rexiati Maimaiti Chaoqun Zhu Yanghai Zhang Qiyue Ding Wei Guo RBM20-Mediated Pre-mRNA Splicing Has Muscle-Specificity and Differential Hormonal Responses between Muscles and in Muscle Cell Cultures International Journal of Molecular Sciences RBM20 pre-mRNA splicing titin hormones skeletal muscle |
author_facet |
Rexiati Maimaiti Chaoqun Zhu Yanghai Zhang Qiyue Ding Wei Guo |
author_sort |
Rexiati Maimaiti |
title |
RBM20-Mediated Pre-mRNA Splicing Has Muscle-Specificity and Differential Hormonal Responses between Muscles and in Muscle Cell Cultures |
title_short |
RBM20-Mediated Pre-mRNA Splicing Has Muscle-Specificity and Differential Hormonal Responses between Muscles and in Muscle Cell Cultures |
title_full |
RBM20-Mediated Pre-mRNA Splicing Has Muscle-Specificity and Differential Hormonal Responses between Muscles and in Muscle Cell Cultures |
title_fullStr |
RBM20-Mediated Pre-mRNA Splicing Has Muscle-Specificity and Differential Hormonal Responses between Muscles and in Muscle Cell Cultures |
title_full_unstemmed |
RBM20-Mediated Pre-mRNA Splicing Has Muscle-Specificity and Differential Hormonal Responses between Muscles and in Muscle Cell Cultures |
title_sort |
rbm20-mediated pre-mrna splicing has muscle-specificity and differential hormonal responses between muscles and in muscle cell cultures |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-03-01 |
description |
Pre-mRNA splicing plays an important role in muscle function and diseases. The RNA binding motif 20 (RBM20) is a splicing factor that is predominantly expressed in muscle tissues and primarily regulates pre-mRNA splicing of Ttn, encoding a giant muscle protein titin that is responsible for muscle function and diseases. RBM20-mediated Ttn splicing has been mostly studied in heart muscle, but not in skeletal muscle. In this study, we investigated splicing specificity in different muscle types in Rbm20 knockout rats and hormonal effects on RBM20-mediated splicing both in cellulo and in vivo studies. The results revealed that RBM20 is differentially expressed across muscles and RBM20-mediated splicing is muscle-type specific. In the presence of RBM20, Ttn splicing responds to hormones in a muscle-type dependent manner, while in the absence of RBM20, Ttn splicing is not affected by hormones. In differentiated and undifferentiated C2C12 cells, RBM20-mediated splicing in response to hormonal effects is mainly through genomic signaling pathway. The knowledge gained from this study may help further understand muscle-specific gene splicing in response to hormone stimuli in different muscle types. |
topic |
RBM20 pre-mRNA splicing titin hormones skeletal muscle |
url |
https://www.mdpi.com/1422-0067/22/6/2928 |
work_keys_str_mv |
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