FEASIBILITY OF HYGROMYCIN AS A SELECTION AGENT IN AGROBACTERIUM-MEDIATED TRANSFORMATION OF OILSEED RAPE (BRASSICA NAPUS L.)
In this work the feasibility of the antibiotic hygromycin as a selection agent in Agrobacterium-mediated transformation of oilseed rape (Brassica napus L.) was evaluated. For this, two economically important commercial varieties Haydn and Hunter and tobacco as a model plant were subjected to Agrobac...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Slovak University of Agriculture
2014-02-01
|
Series: | Journal of Microbiology, Biotechnology and Food Sciences |
Subjects: | |
Online Access: | http://www.jmbfs.org/wp-content/uploads/2014/01/22_jmbfs_hlozakova_2014_b.pdf |
id |
doaj-f4fa69533f8f4c6eadd60054144d7ac2 |
---|---|
record_format |
Article |
spelling |
doaj-f4fa69533f8f4c6eadd60054144d7ac22020-11-25T00:27:55ZengSlovak University of AgricultureJournal of Microbiology, Biotechnology and Food Sciences1338-51782014-02-01vol. 3special issue 2 (Biotechnology)80833032-1FEASIBILITY OF HYGROMYCIN AS A SELECTION AGENT IN AGROBACTERIUM-MEDIATED TRANSFORMATION OF OILSEED RAPE (BRASSICA NAPUS L.)Tímea Kuťka HlozákováZuzana PolóniováJana MoravčíkováIn this work the feasibility of the antibiotic hygromycin as a selection agent in Agrobacterium-mediated transformation of oilseed rape (Brassica napus L.) was evaluated. For this, two economically important commercial varieties Haydn and Hunter and tobacco as a model plant were subjected to Agrobacterium-mediated transformation. The 5-6 days-old oilseed rape hypocotyls and 4-6 weeks-old tobacco leaf segments were transformed with the binary vector pCambia1304. The T-DNA contained the reporter gfp:gus and the selectable marker htp genes. Regeneration of transformed cells was conducted under selection of 10 mg.l-1 (oilseed rape) and 30 mg.l-1 (tobacco) hygromycin. Putative transgenic plantlets were analysed by the mean of the histochemical GUS and PCR analyses. Transformation efficiency ranged from 1.0% (cv. Haydn) to 40.4% (tobacco). No transgenic shoots were detected for the cv. Hunter. It points out the oilseed rape cultivar specificity plays significant role in choice of suitable selection agent.http://www.jmbfs.org/wp-content/uploads/2014/01/22_jmbfs_hlozakova_2014_b.pdfAgrobacterium tumefaciensBrassica napus L.β-glucuronidase genehygromycinoilseed rapetobacco |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tímea Kuťka Hlozáková Zuzana Polóniová Jana Moravčíková |
spellingShingle |
Tímea Kuťka Hlozáková Zuzana Polóniová Jana Moravčíková FEASIBILITY OF HYGROMYCIN AS A SELECTION AGENT IN AGROBACTERIUM-MEDIATED TRANSFORMATION OF OILSEED RAPE (BRASSICA NAPUS L.) Journal of Microbiology, Biotechnology and Food Sciences Agrobacterium tumefaciens Brassica napus L. β-glucuronidase gene hygromycin oilseed rape tobacco |
author_facet |
Tímea Kuťka Hlozáková Zuzana Polóniová Jana Moravčíková |
author_sort |
Tímea Kuťka Hlozáková |
title |
FEASIBILITY OF HYGROMYCIN AS A SELECTION AGENT IN AGROBACTERIUM-MEDIATED TRANSFORMATION OF OILSEED RAPE (BRASSICA NAPUS L.) |
title_short |
FEASIBILITY OF HYGROMYCIN AS A SELECTION AGENT IN AGROBACTERIUM-MEDIATED TRANSFORMATION OF OILSEED RAPE (BRASSICA NAPUS L.) |
title_full |
FEASIBILITY OF HYGROMYCIN AS A SELECTION AGENT IN AGROBACTERIUM-MEDIATED TRANSFORMATION OF OILSEED RAPE (BRASSICA NAPUS L.) |
title_fullStr |
FEASIBILITY OF HYGROMYCIN AS A SELECTION AGENT IN AGROBACTERIUM-MEDIATED TRANSFORMATION OF OILSEED RAPE (BRASSICA NAPUS L.) |
title_full_unstemmed |
FEASIBILITY OF HYGROMYCIN AS A SELECTION AGENT IN AGROBACTERIUM-MEDIATED TRANSFORMATION OF OILSEED RAPE (BRASSICA NAPUS L.) |
title_sort |
feasibility of hygromycin as a selection agent in agrobacterium-mediated transformation of oilseed rape (brassica napus l.) |
publisher |
Slovak University of Agriculture |
series |
Journal of Microbiology, Biotechnology and Food Sciences |
issn |
1338-5178 |
publishDate |
2014-02-01 |
description |
In this work the feasibility of the antibiotic hygromycin as a selection agent in Agrobacterium-mediated transformation of oilseed rape (Brassica napus L.) was evaluated. For this, two economically important commercial varieties Haydn and Hunter and tobacco as a model plant were subjected to Agrobacterium-mediated transformation. The 5-6 days-old oilseed rape hypocotyls and 4-6 weeks-old tobacco leaf segments were transformed with the binary vector pCambia1304. The T-DNA contained the reporter gfp:gus and the selectable marker htp genes. Regeneration of transformed cells was conducted under selection of 10 mg.l-1 (oilseed rape) and 30 mg.l-1 (tobacco) hygromycin. Putative transgenic plantlets were analysed by the mean of the histochemical GUS and PCR analyses. Transformation efficiency ranged from 1.0% (cv. Haydn) to 40.4% (tobacco). No transgenic shoots were detected for the cv. Hunter. It points out the oilseed rape cultivar specificity plays significant role in choice of suitable selection agent. |
topic |
Agrobacterium tumefaciens Brassica napus L. β-glucuronidase gene hygromycin oilseed rape tobacco |
url |
http://www.jmbfs.org/wp-content/uploads/2014/01/22_jmbfs_hlozakova_2014_b.pdf |
work_keys_str_mv |
AT timeakutkahlozakova feasibilityofhygromycinasaselectionagentinagrobacteriummediatedtransformationofoilseedrapebrassicanapusl AT zuzanapoloniova feasibilityofhygromycinasaselectionagentinagrobacteriummediatedtransformationofoilseedrapebrassicanapusl AT janamoravcikova feasibilityofhygromycinasaselectionagentinagrobacteriummediatedtransformationofoilseedrapebrassicanapusl |
_version_ |
1725337710170734592 |