Effectiveness of arbuscular mycorrhizal fungi in the protection of olive plants against oxidative stress induced by drought
<p>Olive trees are often subjected to a long dry season with low water availability which induces oxidative stress. The present study was conducted to evaluate the effectiveness of native <em>Rhizophagus</em> <em>manihotis</em> (Rma) and non-native <em>Funneliform...
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doaj-f54251a8a61a438babb097f7bd2fce0c2020-11-24T20:58:47ZengInstituto Nacional de Investigación y Tecnología Agraria y AlimentariaSpanish Journal of Agricultural Research2171-92922014-06-0112376377110.5424/sjar/2014123-48152024Effectiveness of arbuscular mycorrhizal fungi in the protection of olive plants against oxidative stress induced by droughtMohamed O. Fouad0Abdellatif Essahibi1Laila Benhiba2Ahmed Qaddoury3Plant Biotechnology and Agrophysiology of Symbiosis, Faculty of Sciences and Techniques, University Cadi Ayyad. PO Box 549, Gueliz, MarrakeshPlant Biotechnology and Agrophysiology of Symbiosis, Faculty of Sciences and Techniques, University Cadi Ayyad. PO Box 549, Gueliz, MarrakeshPlant Biotechnology and Agrophysiology of Symbiosis, Faculty of Sciences and Techniques, University Cadi Ayyad. PO Box 549, Gueliz, MarrakeshPlant Biotechnology and Agrophysiology of Symbiosis, Faculty of Sciences and Techniques, University Cadi Ayyad. PO Box 549, Gueliz, Marrakesh<p>Olive trees are often subjected to a long dry season with low water availability which induces oxidative stress. The present study was conducted to evaluate the effectiveness of native <em>Rhizophagus</em> <em>manihotis</em> (Rma) and non-native <em>Funneliformis mosseae</em> (Fmo) arbuscular mycorrhizal (AM) fungi (AMF) in enhancing olive protection against oxidative stress induced by water deficit. Olive plantlets, cv. Picholine marocaine, were inoculated (AM-plants) or not (NM-plants) with Rma<em> </em>or<em> </em>Fmo and subjected to well-watered (75% of field capacity) or water-stressed (25% of field capacity) conditions. After two months, obtained results showed that water stress significantly decreased growth and biomass production of NM-plants, but AMF alleviated the detrimental effects of water deficit on the growth of olive plants. Inoculation with Rma<em> </em>increased<strong> </strong>shoot height by 120%, root length by 56%, fresh weight by 170% (shoot) and 210% (root), and dry weight by 220% (shoot) and 220% (root) compared to NM-plants. AM colonization enhanced drought tolerance in terms of protection against oxidative stress. Mycorrhizal plants showed lower levels of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), malondialdehyde (MDA) and electrolyte leakage (EL) than NM-plants. Rma colonization decreased two times H<sub>2</sub>O<sub>2</sub>, 2.6 times MDA and two times EL levels compared to NM-plants. This protective effect seems to be due to the enhanced activities of superoxide dismutase (534 U mg<sup>-1 </sup>protein), catalase (298 U mg<sup>-1 </sup>protein), guaiacol peroxidase (47 U mg<sup>-1 </sup>protein), and ascorbate peroxidase (305 U mg<sup>-1 </sup>protein) which were highest in Rma-plants. Moreover, Rma-plants showed the lowest oxidative damage to lipid and highest soluble protein content. Thus, the native AMF Rma ought to be considered as a biological tool for enhancing olive tolerance to drought.</p>http://revistas.inia.es/index.php/sjar/article/view/4815mycorrhizal colonizationOlea europaeawater deficitantioxidant activitydrought tolerance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohamed O. Fouad Abdellatif Essahibi Laila Benhiba Ahmed Qaddoury |
spellingShingle |
Mohamed O. Fouad Abdellatif Essahibi Laila Benhiba Ahmed Qaddoury Effectiveness of arbuscular mycorrhizal fungi in the protection of olive plants against oxidative stress induced by drought Spanish Journal of Agricultural Research mycorrhizal colonization Olea europaea water deficit antioxidant activity drought tolerance |
author_facet |
Mohamed O. Fouad Abdellatif Essahibi Laila Benhiba Ahmed Qaddoury |
author_sort |
Mohamed O. Fouad |
title |
Effectiveness of arbuscular mycorrhizal fungi in the protection of olive plants against oxidative stress induced by drought |
title_short |
Effectiveness of arbuscular mycorrhizal fungi in the protection of olive plants against oxidative stress induced by drought |
title_full |
Effectiveness of arbuscular mycorrhizal fungi in the protection of olive plants against oxidative stress induced by drought |
title_fullStr |
Effectiveness of arbuscular mycorrhizal fungi in the protection of olive plants against oxidative stress induced by drought |
title_full_unstemmed |
Effectiveness of arbuscular mycorrhizal fungi in the protection of olive plants against oxidative stress induced by drought |
title_sort |
effectiveness of arbuscular mycorrhizal fungi in the protection of olive plants against oxidative stress induced by drought |
publisher |
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria |
series |
Spanish Journal of Agricultural Research |
issn |
2171-9292 |
publishDate |
2014-06-01 |
description |
<p>Olive trees are often subjected to a long dry season with low water availability which induces oxidative stress. The present study was conducted to evaluate the effectiveness of native <em>Rhizophagus</em> <em>manihotis</em> (Rma) and non-native <em>Funneliformis mosseae</em> (Fmo) arbuscular mycorrhizal (AM) fungi (AMF) in enhancing olive protection against oxidative stress induced by water deficit. Olive plantlets, cv. Picholine marocaine, were inoculated (AM-plants) or not (NM-plants) with Rma<em> </em>or<em> </em>Fmo and subjected to well-watered (75% of field capacity) or water-stressed (25% of field capacity) conditions. After two months, obtained results showed that water stress significantly decreased growth and biomass production of NM-plants, but AMF alleviated the detrimental effects of water deficit on the growth of olive plants. Inoculation with Rma<em> </em>increased<strong> </strong>shoot height by 120%, root length by 56%, fresh weight by 170% (shoot) and 210% (root), and dry weight by 220% (shoot) and 220% (root) compared to NM-plants. AM colonization enhanced drought tolerance in terms of protection against oxidative stress. Mycorrhizal plants showed lower levels of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), malondialdehyde (MDA) and electrolyte leakage (EL) than NM-plants. Rma colonization decreased two times H<sub>2</sub>O<sub>2</sub>, 2.6 times MDA and two times EL levels compared to NM-plants. This protective effect seems to be due to the enhanced activities of superoxide dismutase (534 U mg<sup>-1 </sup>protein), catalase (298 U mg<sup>-1 </sup>protein), guaiacol peroxidase (47 U mg<sup>-1 </sup>protein), and ascorbate peroxidase (305 U mg<sup>-1 </sup>protein) which were highest in Rma-plants. Moreover, Rma-plants showed the lowest oxidative damage to lipid and highest soluble protein content. Thus, the native AMF Rma ought to be considered as a biological tool for enhancing olive tolerance to drought.</p> |
topic |
mycorrhizal colonization Olea europaea water deficit antioxidant activity drought tolerance |
url |
http://revistas.inia.es/index.php/sjar/article/view/4815 |
work_keys_str_mv |
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