Klotho : Expression and Regulation at the Maternal-Conceptus Interface in Pigs
Klotho (KL) is a single transmembrane protein composed of KL1 and KL2 repeats possessing β-glucuronidase activity and maintains calcium homeostasis in physiological state. It has been implicated in pigs that calcium is important for the establishment and maintenance of pregnancy, and our previous st...
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The Korean Society of Animal Reproduction and Biotechnology
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doaj-f55001c9423d440aac6c42b9b48e7e5e2021-01-10T11:03:23ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632014-12-0129437538310.12750/JET.2014.29.4.375Klotho : Expression and Regulation at the Maternal-Conceptus Interface in PigsYohan Choi0Heewon Seo1Jangsoo Shim2Sang-Hwan Hyun3Eunsong Lee4Hakhyun Ka5Division of Biological Science and Technology and IPAID, Yonsei University, Wonju 220-710, Republic of KoreaDivision of Biological Science and Technology and IPAID, Yonsei University, Wonju 220-710, Republic of KoreaDivision of Biological Science and Technology and IPAID, Yonsei University, Wonju 220-710, Republic of KorLaboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of KoreaLaboratory of Theriogenology, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, Republic of KDivision of Biological Science and Technology and IPAID, Yonsei University, Wonju 220-710, Republic of KoreaKlotho (KL) is a single transmembrane protein composed of KL1 and KL2 repeats possessing β-glucuronidase activity and maintains calcium homeostasis in physiological state. It has been implicated in pigs that calcium is important for the establishment and maintenance of pregnancy, and our previous study has shown that transient receptor potential vanilloid type 6 (TRPV6), a calcium ion transporter, is predominantly expressed in the uterine endometrium during pregnancy in pigs. However, expression and function of KL in the uterine endometrium has not been determined in pigs. Thus, the present study determined expression and regulation of KL in the uterine endometrium during the estrous cycle and pregnancy in pigs. Real-time RT-PCR analysis showed that levels of KL mRNA decreased between Days 12 to 15 of the estrous cycle, and its expression showed a biphasic manner during pregnancy. KL mRNA was expressed in conceptuses and in chorioallantoic tissues during pregnancy. Explant culture study showed that expression levels of KL were not affected by treatment of steroid hormones or interleukin-1beta during the implantation period. Furthermore, levels of KL mRNA in the uterine endometrium from gilts carrying somatic cell nuclear transfer (SCNT)- derived embryos were significantly lower than those from gilts carrying natural mating-derived embryos on Day 12 of pregnancy. These results exhibited that KL was expressed at the maternal-conceptus interface in a pregnancy status- and stage-specific manner, and its expression was affected by SCNT procedure, suggesting that KL may play an important role in the establishment and maintenance of pregnancy in pigshttp://www.e-jarb.org/journal/view.html?uid=1454&vmd=Fullpiguterusklendometriumcalcium |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yohan Choi Heewon Seo Jangsoo Shim Sang-Hwan Hyun Eunsong Lee Hakhyun Ka |
spellingShingle |
Yohan Choi Heewon Seo Jangsoo Shim Sang-Hwan Hyun Eunsong Lee Hakhyun Ka Klotho : Expression and Regulation at the Maternal-Conceptus Interface in Pigs Journal of Animal Reproduction and Biotechnology pig uterus kl endometrium calcium |
author_facet |
Yohan Choi Heewon Seo Jangsoo Shim Sang-Hwan Hyun Eunsong Lee Hakhyun Ka |
author_sort |
Yohan Choi |
title |
Klotho : Expression and Regulation at the Maternal-Conceptus Interface in Pigs |
title_short |
Klotho : Expression and Regulation at the Maternal-Conceptus Interface in Pigs |
title_full |
Klotho : Expression and Regulation at the Maternal-Conceptus Interface in Pigs |
title_fullStr |
Klotho : Expression and Regulation at the Maternal-Conceptus Interface in Pigs |
title_full_unstemmed |
Klotho : Expression and Regulation at the Maternal-Conceptus Interface in Pigs |
title_sort |
klotho : expression and regulation at the maternal-conceptus interface in pigs |
publisher |
The Korean Society of Animal Reproduction and Biotechnology |
series |
Journal of Animal Reproduction and Biotechnology |
issn |
2671-4639 2671-4663 |
publishDate |
2014-12-01 |
description |
Klotho (KL) is a single transmembrane protein composed of KL1 and KL2 repeats possessing β-glucuronidase activity and maintains calcium homeostasis in physiological state. It has been implicated in pigs that calcium is important for the establishment and maintenance of pregnancy, and our previous study has shown that transient receptor potential vanilloid type 6 (TRPV6), a calcium ion transporter, is predominantly expressed in the uterine endometrium during pregnancy in pigs. However, expression and function of KL in the uterine endometrium has not been determined in pigs. Thus, the present study determined expression and regulation of KL in the uterine endometrium during the estrous cycle and pregnancy in pigs. Real-time RT-PCR analysis showed that levels of KL mRNA decreased between Days 12 to 15 of the estrous cycle, and its expression showed a biphasic manner during pregnancy. KL mRNA was expressed in conceptuses and in chorioallantoic tissues during pregnancy. Explant culture study showed that expression levels of KL were not affected by treatment of steroid hormones or interleukin-1beta during the implantation period. Furthermore, levels of KL mRNA in the uterine endometrium from gilts carrying somatic cell nuclear transfer (SCNT)- derived embryos were significantly lower than those from gilts carrying natural mating-derived embryos on Day 12 of pregnancy. These results exhibited that KL was expressed at the maternal-conceptus interface in a pregnancy status- and stage-specific manner, and its expression was affected by SCNT procedure, suggesting that KL may play an important role in the establishment and maintenance of pregnancy in pigs |
topic |
pig uterus kl endometrium calcium |
url |
http://www.e-jarb.org/journal/view.html?uid=1454&vmd=Full |
work_keys_str_mv |
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