N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in Trichosporon asahii
Abstract Objective Trichosporon asahii is the major causative fungus of disseminated or deep-seated trichosporonosis and forms a biofilm on medical devices. Biofilm formation leads to antifungal drug resistance, so biofilm-related infections are relatively difficult to treat and infected devices oft...
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doaj-f554c678374242288a2fc8a3c8394a332020-11-25T01:30:04ZengBMCBMC Research Notes1756-05002020-03-011311610.1186/s13104-020-04990-xN,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in Trichosporon asahiiSanae Kurakado0Ryota Chiba1Chisato Sato2Yasuhiko Matsumoto3Takashi Sugita4Department of Microbiology, Meiji Pharmaceutical UniversityDepartment of Microbiology, Meiji Pharmaceutical UniversityDepartment of Microbiology, Meiji Pharmaceutical UniversityDepartment of Microbiology, Meiji Pharmaceutical UniversityDepartment of Microbiology, Meiji Pharmaceutical UniversityAbstract Objective Trichosporon asahii is the major causative fungus of disseminated or deep-seated trichosporonosis and forms a biofilm on medical devices. Biofilm formation leads to antifungal drug resistance, so biofilm-related infections are relatively difficult to treat and infected devices often require surgical removal. Therefore, prevention of biofilm formation is important in clinical settings. In this study, to identify metal cations that affect biofilm formation, we evaluated the effects of cation chelators on biofilm formation in T. asahii. Results We evaluated the effect of cation chelators on biofilm formation, since microorganisms must assimilate essential nutrients from their hosts to form and maintain biofilms. The inhibition by N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) was greater than those by other cation chelators, such as deferoxamine, triethylenetetramine, and ethylenediaminetetraacetic acid. The inhibitory effect of TPEN was suppressed by the addition of zinc. TPEN also inhibited T. asahii hyphal formation, which is related to biofilm formation, and the inhibition was suppressed by the addition of zinc. These results suggest that zinc is essential for biofilm formation and hyphal formation. Thus, zinc chelators have the potential to be developed into a new treatment for biofilm-related infection caused by T. asahii.http://link.springer.com/article/10.1186/s13104-020-04990-xBiofilmChelatorN,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamineTrichosporon asahii |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sanae Kurakado Ryota Chiba Chisato Sato Yasuhiko Matsumoto Takashi Sugita |
spellingShingle |
Sanae Kurakado Ryota Chiba Chisato Sato Yasuhiko Matsumoto Takashi Sugita N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in Trichosporon asahii BMC Research Notes Biofilm Chelator N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine Trichosporon asahii |
author_facet |
Sanae Kurakado Ryota Chiba Chisato Sato Yasuhiko Matsumoto Takashi Sugita |
author_sort |
Sanae Kurakado |
title |
N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in Trichosporon asahii |
title_short |
N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in Trichosporon asahii |
title_full |
N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in Trichosporon asahii |
title_fullStr |
N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in Trichosporon asahii |
title_full_unstemmed |
N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in Trichosporon asahii |
title_sort |
n,n,n′,n′-tetrakis(2-pyridylmethyl)ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in trichosporon asahii |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2020-03-01 |
description |
Abstract Objective Trichosporon asahii is the major causative fungus of disseminated or deep-seated trichosporonosis and forms a biofilm on medical devices. Biofilm formation leads to antifungal drug resistance, so biofilm-related infections are relatively difficult to treat and infected devices often require surgical removal. Therefore, prevention of biofilm formation is important in clinical settings. In this study, to identify metal cations that affect biofilm formation, we evaluated the effects of cation chelators on biofilm formation in T. asahii. Results We evaluated the effect of cation chelators on biofilm formation, since microorganisms must assimilate essential nutrients from their hosts to form and maintain biofilms. The inhibition by N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) was greater than those by other cation chelators, such as deferoxamine, triethylenetetramine, and ethylenediaminetetraacetic acid. The inhibitory effect of TPEN was suppressed by the addition of zinc. TPEN also inhibited T. asahii hyphal formation, which is related to biofilm formation, and the inhibition was suppressed by the addition of zinc. These results suggest that zinc is essential for biofilm formation and hyphal formation. Thus, zinc chelators have the potential to be developed into a new treatment for biofilm-related infection caused by T. asahii. |
topic |
Biofilm Chelator N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine Trichosporon asahii |
url |
http://link.springer.com/article/10.1186/s13104-020-04990-x |
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