Effects of Fruit and Vegetable Low Molecular Mass Fractions on Gene Expression in Gingival Cells Challenged with Prevotella intermedia and Actinomyces naeslundii

Effects of Fruit and Vegetable Low Molecular Mass Fractions on Gene Expression in Gingival Cells Challenged with Prevotella intermedia and Actinomyces naeslundii

Low molecular mass (LMM) fractions obtained from extracts of raspberry, red chicory, and Shiitake mushrooms have been shown to be an useful source of specific antibacterial, antiadhesion/coaggregation, and antibiofilm agent(s) that might be used for protection towards caries and gingivitis. In this...

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Main Authors: Laura Canesi, Cristina Borghi, Monica Stauder, Peter Lingström, Adele Papetti, Jonathan Pratten, Caterina Signoretto, David A. Spratt, Mike Wilson, Egija Zaura, Carla Pruzzo
Format: Article
Language:English
Published: Hindawi Limited 2011-01-01
Series:Journal of Biomedicine and Biotechnology
Online Access:http://dx.doi.org/10.1155/2011/230630
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spelling doaj-f569cfa4c5ca442c996778089f087bba2020-11-25T02:19:16ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512011-01-01201110.1155/2011/230630230630Effects of Fruit and Vegetable Low Molecular Mass Fractions on Gene Expression in Gingival Cells Challenged with Prevotella intermedia and Actinomyces naeslundiiLaura Canesi0Cristina Borghi1Monica Stauder2Peter Lingström3Adele Papetti4Jonathan Pratten5Caterina Signoretto6David A. Spratt7Mike Wilson8Egija Zaura9Carla Pruzzo10DIPTERIS, University of Genova, Corso Europa 26, 16132 Genova, ItalyDIPTERIS, University of Genova, Corso Europa 26, 16132 Genova, ItalyDIPTERIS, University of Genova, Corso Europa 26, 16132 Genova, ItalyDepartment of Cariology, Institute of Odontology at Sahlgrenska Academy, University of Gothenburg, P.O. Box 450, 405 30 Gothenburg, SwedenDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKMicrobiology Section, Department of Pathology and Diagnostics, University of Verona, Strada Le Grazie 8, 37134 Verona, ItalyDepartment of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKDepartment of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UKDepartment of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The NetherlandsDIPTERIS, University of Genova, Corso Europa 26, 16132 Genova, ItalyLow molecular mass (LMM) fractions obtained from extracts of raspberry, red chicory, and Shiitake mushrooms have been shown to be an useful source of specific antibacterial, antiadhesion/coaggregation, and antibiofilm agent(s) that might be used for protection towards caries and gingivitis. In this paper, the effects of such LMM fractions on human gingival KB cells exposed to the periodontal pathogens Prevotella intermedia and Actinomyces naeslundii were evaluated. Expression of cytokeratin 18 (CK18) and β4 integrin (β4INT) genes, that are involved in cell proliferation/differentiation and adhesion, and of the antimicrobial peptide β2 defensin (HβD2) in KB cells was increased upon exposure to either live or heat-killed bacteria. All LMM fractions tested prevented or reduced the induction of gene expression by P. intermedia and A. naeslundii depending on the experimental conditions. Overall, the results suggested that LMM fractions could modulate the effects of bacteria associated with periodontal disease in gingival cells.http://dx.doi.org/10.1155/2011/230630
collection DOAJ
language English
format Article
sources DOAJ
author Laura Canesi
Cristina Borghi
Monica Stauder
Peter Lingström
Adele Papetti
Jonathan Pratten
Caterina Signoretto
David A. Spratt
Mike Wilson
Egija Zaura
Carla Pruzzo
spellingShingle Laura Canesi
Cristina Borghi
Monica Stauder
Peter Lingström
Adele Papetti
Jonathan Pratten
Caterina Signoretto
David A. Spratt
Mike Wilson
Egija Zaura
Carla Pruzzo
Effects of Fruit and Vegetable Low Molecular Mass Fractions on Gene Expression in Gingival Cells Challenged with Prevotella intermedia and Actinomyces naeslundii
Journal of Biomedicine and Biotechnology
author_facet Laura Canesi
Cristina Borghi
Monica Stauder
Peter Lingström
Adele Papetti
Jonathan Pratten
Caterina Signoretto
David A. Spratt
Mike Wilson
Egija Zaura
Carla Pruzzo
author_sort Laura Canesi
title Effects of Fruit and Vegetable Low Molecular Mass Fractions on Gene Expression in Gingival Cells Challenged with Prevotella intermedia and Actinomyces naeslundii
title_short Effects of Fruit and Vegetable Low Molecular Mass Fractions on Gene Expression in Gingival Cells Challenged with Prevotella intermedia and Actinomyces naeslundii
title_full Effects of Fruit and Vegetable Low Molecular Mass Fractions on Gene Expression in Gingival Cells Challenged with Prevotella intermedia and Actinomyces naeslundii
title_fullStr Effects of Fruit and Vegetable Low Molecular Mass Fractions on Gene Expression in Gingival Cells Challenged with Prevotella intermedia and Actinomyces naeslundii
title_full_unstemmed Effects of Fruit and Vegetable Low Molecular Mass Fractions on Gene Expression in Gingival Cells Challenged with Prevotella intermedia and Actinomyces naeslundii
title_sort effects of fruit and vegetable low molecular mass fractions on gene expression in gingival cells challenged with prevotella intermedia and actinomyces naeslundii
publisher Hindawi Limited
series Journal of Biomedicine and Biotechnology
issn 1110-7243
1110-7251
publishDate 2011-01-01
description Low molecular mass (LMM) fractions obtained from extracts of raspberry, red chicory, and Shiitake mushrooms have been shown to be an useful source of specific antibacterial, antiadhesion/coaggregation, and antibiofilm agent(s) that might be used for protection towards caries and gingivitis. In this paper, the effects of such LMM fractions on human gingival KB cells exposed to the periodontal pathogens Prevotella intermedia and Actinomyces naeslundii were evaluated. Expression of cytokeratin 18 (CK18) and β4 integrin (β4INT) genes, that are involved in cell proliferation/differentiation and adhesion, and of the antimicrobial peptide β2 defensin (HβD2) in KB cells was increased upon exposure to either live or heat-killed bacteria. All LMM fractions tested prevented or reduced the induction of gene expression by P. intermedia and A. naeslundii depending on the experimental conditions. Overall, the results suggested that LMM fractions could modulate the effects of bacteria associated with periodontal disease in gingival cells.
url http://dx.doi.org/10.1155/2011/230630
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