The effect of exposure of SO in high concentrations on CD19 cells in reactive airway dysfunction syndrome in rat

• Main Authors: Zhiyuan Zhang, Zhuang Ma, Wenwu Sun, Debin Ma, Jianping Cao
• Format: Article
• Language: English
• Published: SAGE Publishing 2018-07-01
• Series: European Journal of Inflammation
• Online Access: https://doi.org/10.1177/2058739218791905

Reactive airway dysfunction syndrome (RADS) has a clinical manifestation similar to asthma, but some features are different between both the diseases. To probe the effect of CD19 + cells in RADS pathogenesis by inhalation of sulfur dioxide (SO 2 ), rats were exposed to SO 2 at 600 ppm for 2 h per day for 7 days and the CD19 expression in lung tissue was detected both at mRNA and protein levels by RT-PCR and western blot. The percentages of CD19 + and CD19 + CD23 + cells were measured by flow cytometry. IgG, IgA, and IgE in serum and bronchoalveolar lavage fluid (BALF) were detected by enzyme-linked immunosorbent assay (ELISA). Histological analysis was performed. The results showed that expression of CD19 in SO 2 exposure group was lower than that in the control both at mRNA and protein levels ( P  < 0.05). Flow cytometry analysis showed that the percentages of CD19 + and CD19 + CD23 + were significantly lower in the SO 2 exposed group than that in the control ( P  < 0.05). There was no difference between the control and SO 2 exposed groups in both serum and BALF levels of IgG, IgA, and IgE. Pathological changes, such as chronic bronchitis, local alveolar hemorrhage, and lymphocytes infiltration were observed in SO 2 exposed. RADS is a non-immunogenicity, chronic airway inflammatory disease caused by irritation of harmful factor and manifests as airway hyperresposiveness.