Automatic Classification of the Movements of Directed and Undirected Subviral Particles

• Main Authors: Kaak Michelle, Rausch Andreas, Schanze Thomas
• Format: Article
• Language: English
• Published: De Gruyter 2020-09-01
• Series: Current Directions in Biomedical Engineering
• Subjects: subviral particles mean squared displacement; fluorescence microscopy; movement patterns
• Online Access: https://doi.org/10.1515/cdbme-2020-3038

The development of drugs against pathogens that cause hemorrhagic fever, such as Marburg and Ebola virus, requires researchers to gather much information about the virus. The accelerating of the research process is of great interest; therefore a new algorithm was developed to analyze intracellular processes. The algorithm will classify the motion characteristics of subviral particles in fluorescence microscopic image sequences of Ebola or Marburg virusinfected cells. The classification is based on the calculation of mean squared displacement. The results look promising to distinguish different particle tracks in active and passive transport. The paper ends with a discussion.