Molecular Spectroscopic Imaging Offers a Systematic Assessment of Pathological Aortic Valve and Prosthesis Tissue in Biomineralization
<b> </b>Pathological ECM remodelling and biomineralization in human aortic valve and bioprosthesis tissue were investigated by Fourier transformed infrared (FT-IR) spectroscopic imaging and multivariate data analysis. Results of histological von Kossa staining to monitor hydroxyapatite b...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-08-01
|
Series: | Crystals |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4352/10/9/763 |
id |
doaj-f58a1b479db34b2383041eef5eb98fed |
---|---|
record_format |
Article |
spelling |
doaj-f58a1b479db34b2383041eef5eb98fed2020-11-25T03:53:12ZengMDPI AGCrystals2073-43522020-08-011076376310.3390/cryst10090763Molecular Spectroscopic Imaging Offers a Systematic Assessment of Pathological Aortic Valve and Prosthesis Tissue in BiomineralizationClaudia Dittfeld0Alice Mieting1Cindy Welzel2Anett Jannasch3Klaus Matschke4Sems-Malte Tugtekin5Gerald Steiner6Department of Cardiac Surgery, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Heart Centre Dresden, Fetscherstrasse 76, 01307 Dresden, GermanyDepartment of Cardiac Surgery, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Heart Centre Dresden, Fetscherstrasse 76, 01307 Dresden, GermanyDepartment of Cardiac Surgery, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Heart Centre Dresden, Fetscherstrasse 76, 01307 Dresden, GermanyDepartment of Cardiac Surgery, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Heart Centre Dresden, Fetscherstrasse 76, 01307 Dresden, GermanyDepartment of Cardiac Surgery, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Heart Centre Dresden, Fetscherstrasse 76, 01307 Dresden, GermanyDepartment of Cardiac Surgery, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Heart Centre Dresden, Fetscherstrasse 76, 01307 Dresden, GermanyClinical Sensoring and Monitoring, Faculty of Medicine Carl Gustav Carus, Dresden University of Technology, 01307 Dresden, Germany<b> </b>Pathological ECM remodelling and biomineralization in human aortic valve and bioprosthesis tissue were investigated by Fourier transformed infrared (FT-IR) spectroscopic imaging and multivariate data analysis. Results of histological von Kossa staining to monitor hydroxyapatite biomineralization correlated to the definition of mineralized tissue using FT-IR spectroscopic imaging. Spectra exhibit signals of carbonate and phosphate groups of hydroxyapatite. Proteins could be identified by the amide I and amide II bands. Proteins were detected in the calcified human aortic valve tissue, but no absorption signals of proteins were observed in the mineralized bioprosthesis sample region. A shift of the amide I band from 1654 cm<sup>−1</sup> to 1636 cm<sup>−1</sup> was assumed to result from β-sheet structures. This band shift was observed in regions where the mineralization process had been identified but also in non-mineralized bioprosthesis tissue independent of prior implantation. The increased occurrence of β-sheet conformation is hypothesized to be a promoter of the biomineralization process. FT-IR spectroscopic imaging offers a wealth of chemical information. For example, slight variations in band position and intensity allow investigation of heterogeneity across aortic valve tissue sections. The exact evaluation of these properties and correlation with conventional histological staining techniques give insights into aortic valve tissue remodelling and calcific pathogenesis.https://www.mdpi.com/2073-4352/10/9/763FT-IR spectroscopyaortic valve tissuebiomineralizationbioprosthesisPCAvon Kossa staining |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Claudia Dittfeld Alice Mieting Cindy Welzel Anett Jannasch Klaus Matschke Sems-Malte Tugtekin Gerald Steiner |
spellingShingle |
Claudia Dittfeld Alice Mieting Cindy Welzel Anett Jannasch Klaus Matschke Sems-Malte Tugtekin Gerald Steiner Molecular Spectroscopic Imaging Offers a Systematic Assessment of Pathological Aortic Valve and Prosthesis Tissue in Biomineralization Crystals FT-IR spectroscopy aortic valve tissue biomineralization bioprosthesis PCA von Kossa staining |
author_facet |
Claudia Dittfeld Alice Mieting Cindy Welzel Anett Jannasch Klaus Matschke Sems-Malte Tugtekin Gerald Steiner |
author_sort |
Claudia Dittfeld |
title |
Molecular Spectroscopic Imaging Offers a Systematic Assessment of Pathological Aortic Valve and Prosthesis Tissue in Biomineralization |
title_short |
Molecular Spectroscopic Imaging Offers a Systematic Assessment of Pathological Aortic Valve and Prosthesis Tissue in Biomineralization |
title_full |
Molecular Spectroscopic Imaging Offers a Systematic Assessment of Pathological Aortic Valve and Prosthesis Tissue in Biomineralization |
title_fullStr |
Molecular Spectroscopic Imaging Offers a Systematic Assessment of Pathological Aortic Valve and Prosthesis Tissue in Biomineralization |
title_full_unstemmed |
Molecular Spectroscopic Imaging Offers a Systematic Assessment of Pathological Aortic Valve and Prosthesis Tissue in Biomineralization |
title_sort |
molecular spectroscopic imaging offers a systematic assessment of pathological aortic valve and prosthesis tissue in biomineralization |
publisher |
MDPI AG |
series |
Crystals |
issn |
2073-4352 |
publishDate |
2020-08-01 |
description |
<b> </b>Pathological ECM remodelling and biomineralization in human aortic valve and bioprosthesis tissue were investigated by Fourier transformed infrared (FT-IR) spectroscopic imaging and multivariate data analysis. Results of histological von Kossa staining to monitor hydroxyapatite biomineralization correlated to the definition of mineralized tissue using FT-IR spectroscopic imaging. Spectra exhibit signals of carbonate and phosphate groups of hydroxyapatite. Proteins could be identified by the amide I and amide II bands. Proteins were detected in the calcified human aortic valve tissue, but no absorption signals of proteins were observed in the mineralized bioprosthesis sample region. A shift of the amide I band from 1654 cm<sup>−1</sup> to 1636 cm<sup>−1</sup> was assumed to result from β-sheet structures. This band shift was observed in regions where the mineralization process had been identified but also in non-mineralized bioprosthesis tissue independent of prior implantation. The increased occurrence of β-sheet conformation is hypothesized to be a promoter of the biomineralization process. FT-IR spectroscopic imaging offers a wealth of chemical information. For example, slight variations in band position and intensity allow investigation of heterogeneity across aortic valve tissue sections. The exact evaluation of these properties and correlation with conventional histological staining techniques give insights into aortic valve tissue remodelling and calcific pathogenesis. |
topic |
FT-IR spectroscopy aortic valve tissue biomineralization bioprosthesis PCA von Kossa staining |
url |
https://www.mdpi.com/2073-4352/10/9/763 |
work_keys_str_mv |
AT claudiadittfeld molecularspectroscopicimagingoffersasystematicassessmentofpathologicalaorticvalveandprosthesistissueinbiomineralization AT alicemieting molecularspectroscopicimagingoffersasystematicassessmentofpathologicalaorticvalveandprosthesistissueinbiomineralization AT cindywelzel molecularspectroscopicimagingoffersasystematicassessmentofpathologicalaorticvalveandprosthesistissueinbiomineralization AT anettjannasch molecularspectroscopicimagingoffersasystematicassessmentofpathologicalaorticvalveandprosthesistissueinbiomineralization AT klausmatschke molecularspectroscopicimagingoffersasystematicassessmentofpathologicalaorticvalveandprosthesistissueinbiomineralization AT semsmaltetugtekin molecularspectroscopicimagingoffersasystematicassessmentofpathologicalaorticvalveandprosthesistissueinbiomineralization AT geraldsteiner molecularspectroscopicimagingoffersasystematicassessmentofpathologicalaorticvalveandprosthesistissueinbiomineralization |
_version_ |
1724479554414706688 |