Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish
The aim of the present study was to test the possible ameliorative efficacy of phytochemicals such as tannins on intestinal inflammation and dysbiosis. The effect of a chestnut shell (<i>Castanea sativa</i>) extract (CSE) rich in polyphenols, mainly represented by tannins, on k-carrageen...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-05-01
|
Series: | Animals |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-2615/11/6/1538 |
id |
doaj-f58db25b89fa4b6193722dfe70e49791 |
---|---|
record_format |
Article |
spelling |
doaj-f58db25b89fa4b6193722dfe70e497912021-06-01T01:01:52ZengMDPI AGAnimals2076-26152021-05-01111538153810.3390/ani11061538Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in ZebrafishGraziella Orso0Mikhail M. Solovyev1Serena Facchiano2Evgeniia Tyrikova3Daniela Sateriale4Elena Kashinskaya5Caterina Pagliarulo6Hossein S. Hoseinifar7Evgeniy Simonov8Ettore Varricchio9Marina Paolucci10Roberta Imperatore11Department of Science and Technology (DST), University of Sannio, 82100 Benevento, ItalyInstitute of Systematics and Ecology of Animals, Siberian Branch of RAS, 630091 Novosibirsk, RussiaDepartment of Science and Technology (DST), University of Sannio, 82100 Benevento, ItalyInstitute of Systematics and Ecology of Animals, Siberian Branch of RAS, 630091 Novosibirsk, RussiaDepartment of Science and Technology (DST), University of Sannio, 82100 Benevento, ItalyInstitute of Systematics and Ecology of Animals, Siberian Branch of RAS, 630091 Novosibirsk, RussiaDepartment of Science and Technology (DST), University of Sannio, 82100 Benevento, ItalyDepartment of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, 49138-15739 Gorgan, IranInstitute of Environmental and Agricultural Biology (X-BIO), University of Tyumen, 625003 Tyumen, RussiaDepartment of Science and Technology (DST), University of Sannio, 82100 Benevento, ItalyDepartment of Science and Technology (DST), University of Sannio, 82100 Benevento, ItalyDepartment of Science and Technology (DST), University of Sannio, 82100 Benevento, ItalyThe aim of the present study was to test the possible ameliorative efficacy of phytochemicals such as tannins on intestinal inflammation and dysbiosis. The effect of a chestnut shell (<i>Castanea sativa</i>) extract (CSE) rich in polyphenols, mainly represented by tannins, on k-carrageenan-induced intestinal inflammation in adult zebrafish (<i>Danio rerio</i>) was tested in a feeding trial. Intestinal inflammation was induced by 0.1% k-carrageenan added to the diet for 10 days. CSE was administered for 10 days after k-carrageenan induced inflammation. The intestinal morphology and histopathology, cytokine expression, and microbiota were analyzed. The k-carrageenan treatment led to gut lumen expansion, reduction of intestinal folds, and increase of the goblet cells number, accompanied by the upregulation of pro-inflammatory factors (TNFα, COX2) and alteration in the number and ratio of taxonomic groups of bacteria. CSE counteracted the inflammatory status enhancing the growth of health helpful bacteria (<i>Enterobacteriaceae</i> and <i>Pseudomonas</i>), decreasing the pro-inflammatory factors, and activating the anti-inflammatory cytokine IL-10. In conclusion, CSE acted as a prebiotic on zebrafish gut microbiota, sustaining the use of tannins as food additives to ameliorate the intestinal inflammation. Our results may be relevant for both aquaculture and medical clinic fields.https://www.mdpi.com/2076-2615/11/6/1538zebrafishintestinal inflammationmicrobiotapolyphenolsprebioticscytokines |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Graziella Orso Mikhail M. Solovyev Serena Facchiano Evgeniia Tyrikova Daniela Sateriale Elena Kashinskaya Caterina Pagliarulo Hossein S. Hoseinifar Evgeniy Simonov Ettore Varricchio Marina Paolucci Roberta Imperatore |
spellingShingle |
Graziella Orso Mikhail M. Solovyev Serena Facchiano Evgeniia Tyrikova Daniela Sateriale Elena Kashinskaya Caterina Pagliarulo Hossein S. Hoseinifar Evgeniy Simonov Ettore Varricchio Marina Paolucci Roberta Imperatore Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish Animals zebrafish intestinal inflammation microbiota polyphenols prebiotics cytokines |
author_facet |
Graziella Orso Mikhail M. Solovyev Serena Facchiano Evgeniia Tyrikova Daniela Sateriale Elena Kashinskaya Caterina Pagliarulo Hossein S. Hoseinifar Evgeniy Simonov Ettore Varricchio Marina Paolucci Roberta Imperatore |
author_sort |
Graziella Orso |
title |
Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish |
title_short |
Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish |
title_full |
Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish |
title_fullStr |
Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish |
title_full_unstemmed |
Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish |
title_sort |
chestnut shell tannins: effects on intestinal inflammation and dysbiosis in zebrafish |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2021-05-01 |
description |
The aim of the present study was to test the possible ameliorative efficacy of phytochemicals such as tannins on intestinal inflammation and dysbiosis. The effect of a chestnut shell (<i>Castanea sativa</i>) extract (CSE) rich in polyphenols, mainly represented by tannins, on k-carrageenan-induced intestinal inflammation in adult zebrafish (<i>Danio rerio</i>) was tested in a feeding trial. Intestinal inflammation was induced by 0.1% k-carrageenan added to the diet for 10 days. CSE was administered for 10 days after k-carrageenan induced inflammation. The intestinal morphology and histopathology, cytokine expression, and microbiota were analyzed. The k-carrageenan treatment led to gut lumen expansion, reduction of intestinal folds, and increase of the goblet cells number, accompanied by the upregulation of pro-inflammatory factors (TNFα, COX2) and alteration in the number and ratio of taxonomic groups of bacteria. CSE counteracted the inflammatory status enhancing the growth of health helpful bacteria (<i>Enterobacteriaceae</i> and <i>Pseudomonas</i>), decreasing the pro-inflammatory factors, and activating the anti-inflammatory cytokine IL-10. In conclusion, CSE acted as a prebiotic on zebrafish gut microbiota, sustaining the use of tannins as food additives to ameliorate the intestinal inflammation. Our results may be relevant for both aquaculture and medical clinic fields. |
topic |
zebrafish intestinal inflammation microbiota polyphenols prebiotics cytokines |
url |
https://www.mdpi.com/2076-2615/11/6/1538 |
work_keys_str_mv |
AT graziellaorso chestnutshelltanninseffectsonintestinalinflammationanddysbiosisinzebrafish AT mikhailmsolovyev chestnutshelltanninseffectsonintestinalinflammationanddysbiosisinzebrafish AT serenafacchiano chestnutshelltanninseffectsonintestinalinflammationanddysbiosisinzebrafish AT evgeniiatyrikova chestnutshelltanninseffectsonintestinalinflammationanddysbiosisinzebrafish AT danielasateriale chestnutshelltanninseffectsonintestinalinflammationanddysbiosisinzebrafish AT elenakashinskaya chestnutshelltanninseffectsonintestinalinflammationanddysbiosisinzebrafish AT caterinapagliarulo chestnutshelltanninseffectsonintestinalinflammationanddysbiosisinzebrafish AT hosseinshoseinifar chestnutshelltanninseffectsonintestinalinflammationanddysbiosisinzebrafish AT evgeniysimonov chestnutshelltanninseffectsonintestinalinflammationanddysbiosisinzebrafish AT ettorevarricchio chestnutshelltanninseffectsonintestinalinflammationanddysbiosisinzebrafish AT marinapaolucci chestnutshelltanninseffectsonintestinalinflammationanddysbiosisinzebrafish AT robertaimperatore chestnutshelltanninseffectsonintestinalinflammationanddysbiosisinzebrafish |
_version_ |
1721413360671522816 |