Genetic diversity of the obligate intracellular bacterium <it>Chlamydophila pneumoniae </it>by genome-wide analysis of single nucleotide polymorphisms: evidence for highly clonal population structure

• Main Authors: Solbach Werner, Schreiber Stefan, Maass Matthias, Rupp Jan, Gutacker Michaela, Ott Stephan, Rattei Thomas, Wirth Thierry, Gieffers Jens
• Format: Article
• Language: English
• Published: BMC 2007-10-01
• Series: BMC Genomics
• Online Access: http://www.biomedcentral.com/1471-2164/8/355

<p>Abstract</p> <p>Background</p> <p><it>Chlamydophila pneumoniae </it>is an obligate intracellular bacterium that replicates in a biphasic life cycle within eukaryotic host cells. Four published genomes revealed an identity of > 99 %. This remarkable finding raised questions about the existence of distinguishable genotypes in correlation with geographical and anatomical origin.</p> <p>Results</p> <p>We studied the genetic diversity of <it>C. pneumoniae </it>by analysing synonymous single nucleotide polymorphisms (sSNPs) that are under reduced selection pressure. We conducted an in silico analysis of the four sequenced genomes, chose 232 representative sSNPs and analysed the loci in 38 <it>C. pneumoniae </it>isolates. We identified 15 different genotypes that were separated in four major clusters. Clusters were not associated with anatomical or geographical origin. However, animal lineages are basal on the <it>C. pneumomiae </it>phylogeny, suggesting a recent transmission to humans through successive bottlenecks some 150,000 years ago. A lack of detectable variation in 17 isolates emphasizes the extraordinary genetic conservation of this species and the high clonality of the population. Moreover, the largest cluster, which encompasses 80% of all analysed strains, is an extremely young clade, that went through an important population expansion some 3,300 years ago.</p> <p>Conclusion</p> <p>sSNPs have proven useful as a sensitive marker to gain new insights into genetic diversity, population structure and evolutionary history of <it>C. pneumoniae</it>.</p>