Differential gene expression in foxtail millet during incompatible interaction with Uromyces setariae-italicae.
Foxtail millet (Setaria italica) is an important food and fodder grain crop that is grown for human consumption. Production of this species is affected by several plant diseases, such as rust. The cultivar Shilixiang has been identified as resistant to the foxtail millet rust pathogen, Uromyces seta...
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doaj-f5b5850f6c2141c39920d1443ed780442020-11-24T21:23:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012382510.1371/journal.pone.0123825Differential gene expression in foxtail millet during incompatible interaction with Uromyces setariae-italicae.Zhi Yong LiNan WangLi DongHui BaiJian Zhang QuanLei LiuZhi-Ping DongFoxtail millet (Setaria italica) is an important food and fodder grain crop that is grown for human consumption. Production of this species is affected by several plant diseases, such as rust. The cultivar Shilixiang has been identified as resistant to the foxtail millet rust pathogen, Uromyces setariae-italicae. In order to identify signaling pathways and genes related to the plant's defense mechanisms against rust, the Shilixiang cultivar was used to construct a digital gene expression (DGE) library during the interaction of foxtail millet with U. setariae-italicae. In this study, we determined the most abundant differentially expressed signaling pathways of up-regulated genes in foxtail millet and identified significantly up-regulated genes. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to analyze the expression of nine selected genes, and the patterns observed agreed well with DGE analysis. Expression levels of the genes were also compared between a resistant cultivar Shilixiang and a susceptible cultivar Yugu-1, and the result indicated that expression level of Shilixiang is higher than that of Yugu-1. This study reveals the relatively comprehensive mechanisms of rust-responsive transcription in foxtail millet.http://europepmc.org/articles/PMC4401669?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhi Yong Li Nan Wang Li Dong Hui Bai Jian Zhang Quan Lei Liu Zhi-Ping Dong |
spellingShingle |
Zhi Yong Li Nan Wang Li Dong Hui Bai Jian Zhang Quan Lei Liu Zhi-Ping Dong Differential gene expression in foxtail millet during incompatible interaction with Uromyces setariae-italicae. PLoS ONE |
author_facet |
Zhi Yong Li Nan Wang Li Dong Hui Bai Jian Zhang Quan Lei Liu Zhi-Ping Dong |
author_sort |
Zhi Yong Li |
title |
Differential gene expression in foxtail millet during incompatible interaction with Uromyces setariae-italicae. |
title_short |
Differential gene expression in foxtail millet during incompatible interaction with Uromyces setariae-italicae. |
title_full |
Differential gene expression in foxtail millet during incompatible interaction with Uromyces setariae-italicae. |
title_fullStr |
Differential gene expression in foxtail millet during incompatible interaction with Uromyces setariae-italicae. |
title_full_unstemmed |
Differential gene expression in foxtail millet during incompatible interaction with Uromyces setariae-italicae. |
title_sort |
differential gene expression in foxtail millet during incompatible interaction with uromyces setariae-italicae. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Foxtail millet (Setaria italica) is an important food and fodder grain crop that is grown for human consumption. Production of this species is affected by several plant diseases, such as rust. The cultivar Shilixiang has been identified as resistant to the foxtail millet rust pathogen, Uromyces setariae-italicae. In order to identify signaling pathways and genes related to the plant's defense mechanisms against rust, the Shilixiang cultivar was used to construct a digital gene expression (DGE) library during the interaction of foxtail millet with U. setariae-italicae. In this study, we determined the most abundant differentially expressed signaling pathways of up-regulated genes in foxtail millet and identified significantly up-regulated genes. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to analyze the expression of nine selected genes, and the patterns observed agreed well with DGE analysis. Expression levels of the genes were also compared between a resistant cultivar Shilixiang and a susceptible cultivar Yugu-1, and the result indicated that expression level of Shilixiang is higher than that of Yugu-1. This study reveals the relatively comprehensive mechanisms of rust-responsive transcription in foxtail millet. |
url |
http://europepmc.org/articles/PMC4401669?pdf=render |
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