Candidate Genes for Yellow Leaf Color in Common Wheat (Triticum aestivum L.) and Major Related Metabolic Pathways according to Transcriptome Profiling
The photosynthetic capacity and efficiency of a crop depends on the biosynthesis of photosynthetic pigments and chloroplast development. However, little is known about the molecular mechanisms of chloroplast development and chlorophyll (Chl) biosynthesis in common wheat because of its huge and compl...
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doaj-f5dc5d15f1634be69ee2d2bf5d90df3c2020-11-25T00:21:38ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-05-01196159410.3390/ijms19061594ijms19061594Candidate Genes for Yellow Leaf Color in Common Wheat (Triticum aestivum L.) and Major Related Metabolic Pathways according to Transcriptome ProfilingHuiyu Wu0Narong Shi1Xuyao An2Cong Liu3Hongfei Fu4Li Cao5Yi Feng6Daojie Sun7Lingli Zhang8College of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, ChinaThe photosynthetic capacity and efficiency of a crop depends on the biosynthesis of photosynthetic pigments and chloroplast development. However, little is known about the molecular mechanisms of chloroplast development and chlorophyll (Chl) biosynthesis in common wheat because of its huge and complex genome. Ygm, a spontaneous yellow-green leaf color mutant of winter wheat, exhibits reduced Chl contents and abnormal chloroplast development. Thus, we searched for candidate genes associated with this phenotype. Comparative transcriptome profiling was performed using leaves from the yellow leaf color type (Y) and normal green color type (G) of the Ygm mutant progeny. We identified 1227 differentially expressed genes (DEGs) in Y compared with G (i.e., 689 upregulated genes and 538 downregulated genes). Gene ontology and pathway enrichment analyses indicated that the DEGs were involved in Chl biosynthesis (i.e., magnesium chelatase subunit H (CHLH) and protochlorophyllide oxidoreductase (POR) genes), carotenoid biosynthesis (i.e., β-carotene hydroxylase (BCH) genes), photosynthesis, and carbon fixation in photosynthetic organisms. We also identified heat shock protein (HSP) genes (sHSP, HSP70, HSP90, and DnaJ) and heat shock transcription factor genes that might have vital roles in chloroplast development. Quantitative RT-PCR analysis of the relevant DEGs confirmed the RNA-Seq results. Moreover, measurements of seven intermediate products involved in Chl biosynthesis and five carotenoid compounds involved in carotenoid-xanthophyll biosynthesis confirmed that CHLH and BCH are vital enzymes for the unusual leaf color phenotype in Y type. These results provide insights into leaf color variation in wheat at the transcriptional level.http://www.mdpi.com/1422-0067/19/6/1594RNA-Seqtranscription factorchlorophyll biosynthesis precursorcarotenoid compositionwheatyellow-green leaf color mutant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huiyu Wu Narong Shi Xuyao An Cong Liu Hongfei Fu Li Cao Yi Feng Daojie Sun Lingli Zhang |
spellingShingle |
Huiyu Wu Narong Shi Xuyao An Cong Liu Hongfei Fu Li Cao Yi Feng Daojie Sun Lingli Zhang Candidate Genes for Yellow Leaf Color in Common Wheat (Triticum aestivum L.) and Major Related Metabolic Pathways according to Transcriptome Profiling International Journal of Molecular Sciences RNA-Seq transcription factor chlorophyll biosynthesis precursor carotenoid composition wheat yellow-green leaf color mutant |
author_facet |
Huiyu Wu Narong Shi Xuyao An Cong Liu Hongfei Fu Li Cao Yi Feng Daojie Sun Lingli Zhang |
author_sort |
Huiyu Wu |
title |
Candidate Genes for Yellow Leaf Color in Common Wheat (Triticum aestivum L.) and Major Related Metabolic Pathways according to Transcriptome Profiling |
title_short |
Candidate Genes for Yellow Leaf Color in Common Wheat (Triticum aestivum L.) and Major Related Metabolic Pathways according to Transcriptome Profiling |
title_full |
Candidate Genes for Yellow Leaf Color in Common Wheat (Triticum aestivum L.) and Major Related Metabolic Pathways according to Transcriptome Profiling |
title_fullStr |
Candidate Genes for Yellow Leaf Color in Common Wheat (Triticum aestivum L.) and Major Related Metabolic Pathways according to Transcriptome Profiling |
title_full_unstemmed |
Candidate Genes for Yellow Leaf Color in Common Wheat (Triticum aestivum L.) and Major Related Metabolic Pathways according to Transcriptome Profiling |
title_sort |
candidate genes for yellow leaf color in common wheat (triticum aestivum l.) and major related metabolic pathways according to transcriptome profiling |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-05-01 |
description |
The photosynthetic capacity and efficiency of a crop depends on the biosynthesis of photosynthetic pigments and chloroplast development. However, little is known about the molecular mechanisms of chloroplast development and chlorophyll (Chl) biosynthesis in common wheat because of its huge and complex genome. Ygm, a spontaneous yellow-green leaf color mutant of winter wheat, exhibits reduced Chl contents and abnormal chloroplast development. Thus, we searched for candidate genes associated with this phenotype. Comparative transcriptome profiling was performed using leaves from the yellow leaf color type (Y) and normal green color type (G) of the Ygm mutant progeny. We identified 1227 differentially expressed genes (DEGs) in Y compared with G (i.e., 689 upregulated genes and 538 downregulated genes). Gene ontology and pathway enrichment analyses indicated that the DEGs were involved in Chl biosynthesis (i.e., magnesium chelatase subunit H (CHLH) and protochlorophyllide oxidoreductase (POR) genes), carotenoid biosynthesis (i.e., β-carotene hydroxylase (BCH) genes), photosynthesis, and carbon fixation in photosynthetic organisms. We also identified heat shock protein (HSP) genes (sHSP, HSP70, HSP90, and DnaJ) and heat shock transcription factor genes that might have vital roles in chloroplast development. Quantitative RT-PCR analysis of the relevant DEGs confirmed the RNA-Seq results. Moreover, measurements of seven intermediate products involved in Chl biosynthesis and five carotenoid compounds involved in carotenoid-xanthophyll biosynthesis confirmed that CHLH and BCH are vital enzymes for the unusual leaf color phenotype in Y type. These results provide insights into leaf color variation in wheat at the transcriptional level. |
topic |
RNA-Seq transcription factor chlorophyll biosynthesis precursor carotenoid composition wheat yellow-green leaf color mutant |
url |
http://www.mdpi.com/1422-0067/19/6/1594 |
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