Population Genetic Diversity and Structure of an Endangered Salicaceae Species in Northeast China: <i>Chosenia arbutifolia</i> (Pall.) A. Skv.
<i>Chosenia arbutifolia</i> (Pall.) A. Skv. is a unique and endangered species belonging to the Salicaceae family. It has great potential for ornamental and industrial use. However, human interference has led to a decrease in and fragmentation of its natural populations in the past two d...
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doaj-f5df5db1402d416785e63cd54fa497b82021-09-26T00:11:00ZengMDPI AGForests1999-49072021-09-01121282128210.3390/f12091282Population Genetic Diversity and Structure of an Endangered Salicaceae Species in Northeast China: <i>Chosenia arbutifolia</i> (Pall.) A. Skv.Yu Wang0Zhongyi Jiao1Jiwei Zheng2Jie Zhou3Baosong Wang4Qiang Zhuge5Xudong He6College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaDepartment of Tree Genetics and Breeding, Jiangsu Academy of Forestry, Nanjing 211153, ChinaDepartment of Tree Genetics and Breeding, Jiangsu Academy of Forestry, Nanjing 211153, ChinaDepartment of Tree Genetics and Breeding, Jiangsu Academy of Forestry, Nanjing 211153, ChinaDepartment of Tree Genetics and Breeding, Jiangsu Academy of Forestry, Nanjing 211153, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaDepartment of Tree Genetics and Breeding, Jiangsu Academy of Forestry, Nanjing 211153, China<i>Chosenia arbutifolia</i> (Pall.) A. Skv. is a unique and endangered species belonging to the Salicaceae family. It has great potential for ornamental and industrial use. However, human interference has led to a decrease in and fragmentation of its natural populations in the past two decades. To effectively evaluate, utilize, and conserve available resources, the genetic diversity and population structure of <i>C. arbutifolia</i> were analyzed in this study. A total of 142 individuals from ten provenances were sampled and sequenced. Moderate diversity was detected among these, with a mean expected heterozygosity and Shannon’s Wiener index of 0.3505 and 0.5258, respectively. The inbreeding coefficient was negative, indicating a significant excess of heterozygotes. The fixation index varied from 0.0068 to 0.3063, showing a varied genetic differentiation between populations. Analysis of molecular variance demonstrated that differentiation accounted for 82.23% of the total variation among individuals, while the remaining 17.77% variation was between populations. Furthermore, the results of population structure analysis indicated that the 142 individuals originated from three primitive groups. To provide genetic information and help design conservation and management strategies, landscape genomics analysis was performed by investigating loci associated with environmental variables. Eighteen SNP markers were associated with altitude and annual average temperature, of which five were ascribed with specific functions. In conclusion, the current study furthers the understanding of <i>C. arbutifolia</i> genetic architecture and provides insights for germplasm protection.https://www.mdpi.com/1999-4907/12/9/1282willow<i>Chosenia arbutifolia</i>genetic diversitypopulation structureSLAF-seq |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu Wang Zhongyi Jiao Jiwei Zheng Jie Zhou Baosong Wang Qiang Zhuge Xudong He |
spellingShingle |
Yu Wang Zhongyi Jiao Jiwei Zheng Jie Zhou Baosong Wang Qiang Zhuge Xudong He Population Genetic Diversity and Structure of an Endangered Salicaceae Species in Northeast China: <i>Chosenia arbutifolia</i> (Pall.) A. Skv. Forests willow <i>Chosenia arbutifolia</i> genetic diversity population structure SLAF-seq |
author_facet |
Yu Wang Zhongyi Jiao Jiwei Zheng Jie Zhou Baosong Wang Qiang Zhuge Xudong He |
author_sort |
Yu Wang |
title |
Population Genetic Diversity and Structure of an Endangered Salicaceae Species in Northeast China: <i>Chosenia arbutifolia</i> (Pall.) A. Skv. |
title_short |
Population Genetic Diversity and Structure of an Endangered Salicaceae Species in Northeast China: <i>Chosenia arbutifolia</i> (Pall.) A. Skv. |
title_full |
Population Genetic Diversity and Structure of an Endangered Salicaceae Species in Northeast China: <i>Chosenia arbutifolia</i> (Pall.) A. Skv. |
title_fullStr |
Population Genetic Diversity and Structure of an Endangered Salicaceae Species in Northeast China: <i>Chosenia arbutifolia</i> (Pall.) A. Skv. |
title_full_unstemmed |
Population Genetic Diversity and Structure of an Endangered Salicaceae Species in Northeast China: <i>Chosenia arbutifolia</i> (Pall.) A. Skv. |
title_sort |
population genetic diversity and structure of an endangered salicaceae species in northeast china: <i>chosenia arbutifolia</i> (pall.) a. skv. |
publisher |
MDPI AG |
series |
Forests |
issn |
1999-4907 |
publishDate |
2021-09-01 |
description |
<i>Chosenia arbutifolia</i> (Pall.) A. Skv. is a unique and endangered species belonging to the Salicaceae family. It has great potential for ornamental and industrial use. However, human interference has led to a decrease in and fragmentation of its natural populations in the past two decades. To effectively evaluate, utilize, and conserve available resources, the genetic diversity and population structure of <i>C. arbutifolia</i> were analyzed in this study. A total of 142 individuals from ten provenances were sampled and sequenced. Moderate diversity was detected among these, with a mean expected heterozygosity and Shannon’s Wiener index of 0.3505 and 0.5258, respectively. The inbreeding coefficient was negative, indicating a significant excess of heterozygotes. The fixation index varied from 0.0068 to 0.3063, showing a varied genetic differentiation between populations. Analysis of molecular variance demonstrated that differentiation accounted for 82.23% of the total variation among individuals, while the remaining 17.77% variation was between populations. Furthermore, the results of population structure analysis indicated that the 142 individuals originated from three primitive groups. To provide genetic information and help design conservation and management strategies, landscape genomics analysis was performed by investigating loci associated with environmental variables. Eighteen SNP markers were associated with altitude and annual average temperature, of which five were ascribed with specific functions. In conclusion, the current study furthers the understanding of <i>C. arbutifolia</i> genetic architecture and provides insights for germplasm protection. |
topic |
willow <i>Chosenia arbutifolia</i> genetic diversity population structure SLAF-seq |
url |
https://www.mdpi.com/1999-4907/12/9/1282 |
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