Characterization of a Plasmodium falciparum mutant that has deleted the majority of the gametocyte-specific Pf11-1 locus
We identified a gametocyte-specific protein of Plasmodium falciparum called Pf11-1 and provide experimental evidence that this molecule is involved in the emergence of gametes of the infected erythrocyte (gametogenesis). A mutant parasite clone, which has deleted over 90% of the PF11-1 gene locus, w...
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Instituto Oswaldo Cruz, Ministério da Saúde
1992-01-01
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doaj-f60f8f62ba934ca6a43c94b624553ffa2020-11-25T00:50:31ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80601992-01-0187919410.1590/S0074-02761992000700012Characterization of a Plasmodium falciparum mutant that has deleted the majority of the gametocyte-specific Pf11-1 locusArtur ScherfCarolyn PetersenRichard CarterPietro AlanoRick NelsonMasamichi AikawaDenise MatteiLuiz Pereira da SilvaJames LeechWe identified a gametocyte-specific protein of Plasmodium falciparum called Pf11-1 and provide experimental evidence that this molecule is involved in the emergence of gametes of the infected erythrocyte (gametogenesis). A mutant parasite clone, which has deleted over 90% of the PF11-1 gene locus, was an important control to establish the gametocyte-specific expression of the Pf11-1. Molecular analysis of the Pf11-1 deletion indicates that it is presumably due a chromosome breakage with subsequent "healing" by the addition of telomeric heptanucleotides. Moreover, similar DNA rearrangements are observed in most of the laboratory isolates during asexual propagation in vitro.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700012chromosome breakage and healinggametocyte specific antigentelomereHRPIHRP IIdeletions |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Artur Scherf Carolyn Petersen Richard Carter Pietro Alano Rick Nelson Masamichi Aikawa Denise Mattei Luiz Pereira da Silva James Leech |
spellingShingle |
Artur Scherf Carolyn Petersen Richard Carter Pietro Alano Rick Nelson Masamichi Aikawa Denise Mattei Luiz Pereira da Silva James Leech Characterization of a Plasmodium falciparum mutant that has deleted the majority of the gametocyte-specific Pf11-1 locus Memórias do Instituto Oswaldo Cruz. chromosome breakage and healing gametocyte specific antigen telomere HRPI HRP II deletions |
author_facet |
Artur Scherf Carolyn Petersen Richard Carter Pietro Alano Rick Nelson Masamichi Aikawa Denise Mattei Luiz Pereira da Silva James Leech |
author_sort |
Artur Scherf |
title |
Characterization of a Plasmodium falciparum mutant that has deleted the majority of the gametocyte-specific Pf11-1 locus |
title_short |
Characterization of a Plasmodium falciparum mutant that has deleted the majority of the gametocyte-specific Pf11-1 locus |
title_full |
Characterization of a Plasmodium falciparum mutant that has deleted the majority of the gametocyte-specific Pf11-1 locus |
title_fullStr |
Characterization of a Plasmodium falciparum mutant that has deleted the majority of the gametocyte-specific Pf11-1 locus |
title_full_unstemmed |
Characterization of a Plasmodium falciparum mutant that has deleted the majority of the gametocyte-specific Pf11-1 locus |
title_sort |
characterization of a plasmodium falciparum mutant that has deleted the majority of the gametocyte-specific pf11-1 locus |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
1992-01-01 |
description |
We identified a gametocyte-specific protein of Plasmodium falciparum called Pf11-1 and provide experimental evidence that this molecule is involved in the emergence of gametes of the infected erythrocyte (gametogenesis). A mutant parasite clone, which has deleted over 90% of the PF11-1 gene locus, was an important control to establish the gametocyte-specific expression of the Pf11-1. Molecular analysis of the Pf11-1 deletion indicates that it is presumably due a chromosome breakage with subsequent "healing" by the addition of telomeric heptanucleotides. Moreover, similar DNA rearrangements are observed in most of the laboratory isolates during asexual propagation in vitro. |
topic |
chromosome breakage and healing gametocyte specific antigen telomere HRPI HRP II deletions |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700012 |
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