Comparative Assessment of Phytochemical Profiles of Comfrey (<i>Symphytum officinale</i> L.) Root Extracts Obtained by Different Extraction Techniques
In this work a comparative study on phytochemical profiles of comfrey root extracts obtained by different extraction approaches has been carried out. Chemical profiles of extracts obtained by supercritical fluid (SFE), pressurized liquid (PLE), and conventional solid/liquid extraction were compared...
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doaj-f646a9480d054900836a1e923513dfbf2020-11-25T02:06:04ZengMDPI AGMolecules1420-30492020-02-0125483710.3390/molecules25040837molecules25040837Comparative Assessment of Phytochemical Profiles of Comfrey (<i>Symphytum officinale</i> L.) Root Extracts Obtained by Different Extraction TechniquesNataša Nastić0Isabel Borrás-Linares1Jesús Lozano-Sánchez2Jaroslava Švarc-Gajić3Antonio Segura-Carretero4Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, SerbiaFunctional Food Research and Development Centre (CIDAF), Health Science Technological Park, Avda. del Conocimiento s/n, Bioregion building, 18016 Granada, SpainFunctional Food Research and Development Centre (CIDAF), Health Science Technological Park, Avda. del Conocimiento s/n, Bioregion building, 18016 Granada, SpainFaculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, SerbiaFunctional Food Research and Development Centre (CIDAF), Health Science Technological Park, Avda. del Conocimiento s/n, Bioregion building, 18016 Granada, SpainIn this work a comparative study on phytochemical profiles of comfrey root extracts obtained by different extraction approaches has been carried out. Chemical profiles of extracts obtained by supercritical fluid (SFE), pressurized liquid (PLE), and conventional solid/liquid extraction were compared and discussed. Phytochemical composition was assessed by high-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) identifying 39 compounds reported for the first time in comfrey root, mainly phenolic acids and fatty acids. The influence of different extraction parameters on phytochemical profiles of <i>S. officinale</i> root was investigated for all applied techniques. PLE and maceration, using alcohol-based solvents (aqueous methanol or ethanol), were shown to be more efficient in the recovery of more polar compounds. Greater numbers of phenolics were best extracted by PLE using 85% EtOH at 63 °C. The use of SFE and 100% acetone for 30 min enabled good recoveries of nonpolar compounds. SFE using 15% EtOH as a cosolvent at 150 bar produced the best recoveries of a significant number of fatty acids. The main compositional differences between extracts obtained by different extraction techniques were assigned to the solvent type. Hence, these results provided comprehensive approaches for treating comfrey root enriched in different phytochemicals, thereby enhancing its bioaccessibility.https://www.mdpi.com/1420-3049/25/4/837comfrey (<i>symphytum officinale</i> l.) rootmacerationplesfehplc-esi-qtof-ms/msphytochemical compounds |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nataša Nastić Isabel Borrás-Linares Jesús Lozano-Sánchez Jaroslava Švarc-Gajić Antonio Segura-Carretero |
spellingShingle |
Nataša Nastić Isabel Borrás-Linares Jesús Lozano-Sánchez Jaroslava Švarc-Gajić Antonio Segura-Carretero Comparative Assessment of Phytochemical Profiles of Comfrey (<i>Symphytum officinale</i> L.) Root Extracts Obtained by Different Extraction Techniques Molecules comfrey (<i>symphytum officinale</i> l.) root maceration ple sfe hplc-esi-qtof-ms/ms phytochemical compounds |
author_facet |
Nataša Nastić Isabel Borrás-Linares Jesús Lozano-Sánchez Jaroslava Švarc-Gajić Antonio Segura-Carretero |
author_sort |
Nataša Nastić |
title |
Comparative Assessment of Phytochemical Profiles of Comfrey (<i>Symphytum officinale</i> L.) Root Extracts Obtained by Different Extraction Techniques |
title_short |
Comparative Assessment of Phytochemical Profiles of Comfrey (<i>Symphytum officinale</i> L.) Root Extracts Obtained by Different Extraction Techniques |
title_full |
Comparative Assessment of Phytochemical Profiles of Comfrey (<i>Symphytum officinale</i> L.) Root Extracts Obtained by Different Extraction Techniques |
title_fullStr |
Comparative Assessment of Phytochemical Profiles of Comfrey (<i>Symphytum officinale</i> L.) Root Extracts Obtained by Different Extraction Techniques |
title_full_unstemmed |
Comparative Assessment of Phytochemical Profiles of Comfrey (<i>Symphytum officinale</i> L.) Root Extracts Obtained by Different Extraction Techniques |
title_sort |
comparative assessment of phytochemical profiles of comfrey (<i>symphytum officinale</i> l.) root extracts obtained by different extraction techniques |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-02-01 |
description |
In this work a comparative study on phytochemical profiles of comfrey root extracts obtained by different extraction approaches has been carried out. Chemical profiles of extracts obtained by supercritical fluid (SFE), pressurized liquid (PLE), and conventional solid/liquid extraction were compared and discussed. Phytochemical composition was assessed by high-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) identifying 39 compounds reported for the first time in comfrey root, mainly phenolic acids and fatty acids. The influence of different extraction parameters on phytochemical profiles of <i>S. officinale</i> root was investigated for all applied techniques. PLE and maceration, using alcohol-based solvents (aqueous methanol or ethanol), were shown to be more efficient in the recovery of more polar compounds. Greater numbers of phenolics were best extracted by PLE using 85% EtOH at 63 °C. The use of SFE and 100% acetone for 30 min enabled good recoveries of nonpolar compounds. SFE using 15% EtOH as a cosolvent at 150 bar produced the best recoveries of a significant number of fatty acids. The main compositional differences between extracts obtained by different extraction techniques were assigned to the solvent type. Hence, these results provided comprehensive approaches for treating comfrey root enriched in different phytochemicals, thereby enhancing its bioaccessibility. |
topic |
comfrey (<i>symphytum officinale</i> l.) root maceration ple sfe hplc-esi-qtof-ms/ms phytochemical compounds |
url |
https://www.mdpi.com/1420-3049/25/4/837 |
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