Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System
Stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a useful strategy. Although MSCs are commonly isolated by exploiting their plastic adherence, several studies have suggested that there are other populations of stem and/or osteoprogenitor cells that are removed from...
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doaj-f65a3a0f982e4d158234d447d7bab82d2020-11-25T03:27:18ZengSAGE PublishingCell Transplantation0963-68971555-38922012-09-012110.3727/096368912X636939Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture SystemSilvia Claros0Noela Rodríguez-Losada1Encarnación Cruz2Enrique Guerado3José Becerra4José A. Andrades Ph.D.5Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), University of Málaga, Málaga, SpainResearch Laboratory, Heart Unit, Virgen de la Victoria University Hospital, Campus de Teatinos, Málaga, SpainHospital Costa del Sol, Division of Orthopaedic Surgery and Traumatology, Marbella, SpainHospital Costa del Sol, Division of Orthopaedic Surgery and Traumatology, Marbella, SpainUniversity of Málaga, BIONAND, Málaga, SpainDepartment of Cell Biology, Genetics and Physiology, Faculty of Sciences, Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), University of Málaga, Málaga, SpainStem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a useful strategy. Although MSCs are commonly isolated by exploiting their plastic adherence, several studies have suggested that there are other populations of stem and/or osteoprogenitor cells that are removed from primary culture during media replacement. Therefore, we developed a three-dimensional (3D) culture system in which adherent and nonadherent stem cells are selected and expanded. Here, we described the characterization of 3D culture-derived cell populations in vitro and the capacity of these cells to differentiate into bone and/or cartilage tissue when placed inside of demineralized bone matrix (DBM) cylinders, implanted subcutaneously into the backs of rat for 2, 4, and 8 weeks. Our results demonstrates that 3D culture cells were a heterogeneous population of uncommitted cells that express pluripotent-, hematopoietic-, mesenchymal-, and endothelial-specific markers in vitro and can undergo osteogenic differentiation in vivo.https://doi.org/10.3727/096368912X636939 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Silvia Claros Noela Rodríguez-Losada Encarnación Cruz Enrique Guerado José Becerra José A. Andrades Ph.D. |
spellingShingle |
Silvia Claros Noela Rodríguez-Losada Encarnación Cruz Enrique Guerado José Becerra José A. Andrades Ph.D. Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System Cell Transplantation |
author_facet |
Silvia Claros Noela Rodríguez-Losada Encarnación Cruz Enrique Guerado José Becerra José A. Andrades Ph.D. |
author_sort |
Silvia Claros |
title |
Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System |
title_short |
Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System |
title_full |
Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System |
title_fullStr |
Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System |
title_full_unstemmed |
Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System |
title_sort |
characterization of adult stem/progenitor cell populations from bone marrow in a three-dimensional collagen gel culture system |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2012-09-01 |
description |
Stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a useful strategy. Although MSCs are commonly isolated by exploiting their plastic adherence, several studies have suggested that there are other populations of stem and/or osteoprogenitor cells that are removed from primary culture during media replacement. Therefore, we developed a three-dimensional (3D) culture system in which adherent and nonadherent stem cells are selected and expanded. Here, we described the characterization of 3D culture-derived cell populations in vitro and the capacity of these cells to differentiate into bone and/or cartilage tissue when placed inside of demineralized bone matrix (DBM) cylinders, implanted subcutaneously into the backs of rat for 2, 4, and 8 weeks. Our results demonstrates that 3D culture cells were a heterogeneous population of uncommitted cells that express pluripotent-, hematopoietic-, mesenchymal-, and endothelial-specific markers in vitro and can undergo osteogenic differentiation in vivo. |
url |
https://doi.org/10.3727/096368912X636939 |
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