Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae
<p class="Pa8"><span class="A1"><span>Microbial secondary metabolites is an important resource for antifungal development in disease control strategy. The objective of this study was to screen <em>Bacillus cereus </em>11UJ, an antagonistic rhizospher...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | Indonesian |
Published: |
Perhimpunan Fitopatologi Indonesia
2015-04-01
|
Series: | Jurnal Fitopatologi Indonesia |
Subjects: | |
Online Access: | http://journal.ipb.ac.id/index.php/jfiti/article/view/9344 |
id |
doaj-f65eeb58ff844ab687545a013e8bf610 |
---|---|
record_format |
Article |
spelling |
doaj-f65eeb58ff844ab687545a013e8bf6102020-11-24T23:07:23ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792015-04-0111210.14692/jfi.11.2.357821Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzaeYadi Suryadi0I Made Samudra1Tri Puji Priyatno2Dwi Ningsih Susilowati3Puji Lestari4Sutoro Sutoro5Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik PertanianBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik PertanianBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik PertanianBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik PertanianBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik PertanianBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian<p class="Pa8"><span class="A1"><span>Microbial secondary metabolites is an important resource for antifungal development in disease control strategy. The objective of this study was to screen <em>Bacillus cereus </em>11UJ, an antagonistic rhizosphere bacteria for potential secondary metabolite production against rice fungal pathogens, i.e. <em>Rhizoctonia solani </em>and <em>Pyricularia oryzae</em>. The antagonistic effect of crude exract was evaluated using sterile filter paper discs on PDA medium. The ethyl acetate extracts of the bacterium showed a better antifungal activity to <em>P. oryzae </em>than those of <em>R. solani. </em>The inhibitory effect of the filtrate proved the potency of the isolates to produce antifungal. Analysis of pyrolysis gas chromatography-mass spectrometry showed that <em>B. cereus </em>11UJ produces 3 major compounds i.e; 9,19-cyclolanostan-3-ol, acetate, (3.beta.)- (CAS) cycloartanyl acetate (13.14%); 4-(2’,2’-dimethyl-6’-methyliden-1’-cyclohexyliden)-3-methyl-2-butanone (9.72%); and stigmast-5-en-3-ol oleat (9.09%) which suggested to play an important role in the suppression of rice fungal pathogens.</span></span></p> <p class="Default"> </p>http://journal.ipb.ac.id/index.php/jfiti/article/view/9344antagonists, gas chromatography mass spectrometry, rice pathogens, secondary metabolytes |
collection |
DOAJ |
language |
Indonesian |
format |
Article |
sources |
DOAJ |
author |
Yadi Suryadi I Made Samudra Tri Puji Priyatno Dwi Ningsih Susilowati Puji Lestari Sutoro Sutoro |
spellingShingle |
Yadi Suryadi I Made Samudra Tri Puji Priyatno Dwi Ningsih Susilowati Puji Lestari Sutoro Sutoro Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae Jurnal Fitopatologi Indonesia antagonists, gas chromatography mass spectrometry, rice pathogens, secondary metabolytes |
author_facet |
Yadi Suryadi I Made Samudra Tri Puji Priyatno Dwi Ningsih Susilowati Puji Lestari Sutoro Sutoro |
author_sort |
Yadi Suryadi |
title |
Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae |
title_short |
Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae |
title_full |
Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae |
title_fullStr |
Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae |
title_full_unstemmed |
Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae |
title_sort |
aktivitas anticendawan bacillus cereus 11uj terhadap rhizoctonia solani dan pyricularia oryzae |
publisher |
Perhimpunan Fitopatologi Indonesia |
series |
Jurnal Fitopatologi Indonesia |
issn |
0215-7950 2339-2479 |
publishDate |
2015-04-01 |
description |
<p class="Pa8"><span class="A1"><span>Microbial secondary metabolites is an important resource for antifungal development in disease control strategy. The objective of this study was to screen <em>Bacillus cereus </em>11UJ, an antagonistic rhizosphere bacteria for potential secondary metabolite production against rice fungal pathogens, i.e. <em>Rhizoctonia solani </em>and <em>Pyricularia oryzae</em>. The antagonistic effect of crude exract was evaluated using sterile filter paper discs on PDA medium. The ethyl acetate extracts of the bacterium showed a better antifungal activity to <em>P. oryzae </em>than those of <em>R. solani. </em>The inhibitory effect of the filtrate proved the potency of the isolates to produce antifungal. Analysis of pyrolysis gas chromatography-mass spectrometry showed that <em>B. cereus </em>11UJ produces 3 major compounds i.e; 9,19-cyclolanostan-3-ol, acetate, (3.beta.)- (CAS) cycloartanyl acetate (13.14%); 4-(2’,2’-dimethyl-6’-methyliden-1’-cyclohexyliden)-3-methyl-2-butanone (9.72%); and stigmast-5-en-3-ol oleat (9.09%) which suggested to play an important role in the suppression of rice fungal pathogens.</span></span></p> <p class="Default"> </p> |
topic |
antagonists, gas chromatography mass spectrometry, rice pathogens, secondary metabolytes |
url |
http://journal.ipb.ac.id/index.php/jfiti/article/view/9344 |
work_keys_str_mv |
AT yadisuryadi aktivitasanticendawanbacilluscereus11ujterhadaprhizoctoniasolanidanpyriculariaoryzae AT imadesamudra aktivitasanticendawanbacilluscereus11ujterhadaprhizoctoniasolanidanpyriculariaoryzae AT tripujipriyatno aktivitasanticendawanbacilluscereus11ujterhadaprhizoctoniasolanidanpyriculariaoryzae AT dwiningsihsusilowati aktivitasanticendawanbacilluscereus11ujterhadaprhizoctoniasolanidanpyriculariaoryzae AT pujilestari aktivitasanticendawanbacilluscereus11ujterhadaprhizoctoniasolanidanpyriculariaoryzae AT sutorosutoro aktivitasanticendawanbacilluscereus11ujterhadaprhizoctoniasolanidanpyriculariaoryzae |
_version_ |
1725618601930522624 |