Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae

<p class="Pa8"><span class="A1"><span>Microbial secondary metabolites is an important resource for antifungal development in disease control strategy. The objective of this study was to screen <em>Bacillus cereus </em>11UJ, an antagonistic rhizospher...

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Main Authors: Yadi Suryadi, I Made Samudra, Tri Puji Priyatno, Dwi Ningsih Susilowati, Puji Lestari, Sutoro Sutoro
Format: Article
Language:Indonesian
Published: Perhimpunan Fitopatologi Indonesia 2015-04-01
Series:Jurnal Fitopatologi Indonesia
Subjects:
Online Access:http://journal.ipb.ac.id/index.php/jfiti/article/view/9344
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spelling doaj-f65eeb58ff844ab687545a013e8bf6102020-11-24T23:07:23ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792015-04-0111210.14692/jfi.11.2.357821Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzaeYadi Suryadi0I Made Samudra1Tri Puji Priyatno2Dwi Ningsih Susilowati3Puji Lestari4Sutoro Sutoro5Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik PertanianBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik PertanianBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik PertanianBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik PertanianBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik PertanianBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian<p class="Pa8"><span class="A1"><span>Microbial secondary metabolites is an important resource for antifungal development in disease control strategy. The objective of this study was to screen <em>Bacillus cereus </em>11UJ, an antagonistic rhizosphere bacteria for potential secondary metabolite production against rice fungal pathogens, i.e. <em>Rhizoctonia solani </em>and <em>Pyricularia oryzae</em>. The antagonistic effect of crude exract was evaluated using sterile filter paper discs on PDA medium. The ethyl acetate extracts of the bacterium showed a better antifungal activity to <em>P. oryzae </em>than those of <em>R. solani. </em>The inhibitory effect of the filtrate proved the potency of the isolates to produce antifungal. Analysis of pyrolysis gas chromatography-mass spectrometry showed that <em>B. cereus </em>11UJ produces 3 major compounds i.e; 9,19-cyclolanostan-3-ol, acetate, (3.beta.)- (CAS) cycloartanyl acetate (13.14%); 4-(2’,2’-dimethyl-6’-methyliden-1’-cyclohexyliden)-3-methyl-2-butanone (9.72%); and stigmast-5-en-3-ol oleat (9.09%) which suggested to play an important role in the suppression of rice fungal pathogens.</span></span></p> <p class="Default"> </p>http://journal.ipb.ac.id/index.php/jfiti/article/view/9344antagonists, gas chromatography mass spectrometry, rice pathogens, secondary metabolytes
collection DOAJ
language Indonesian
format Article
sources DOAJ
author Yadi Suryadi
I Made Samudra
Tri Puji Priyatno
Dwi Ningsih Susilowati
Puji Lestari
Sutoro Sutoro
spellingShingle Yadi Suryadi
I Made Samudra
Tri Puji Priyatno
Dwi Ningsih Susilowati
Puji Lestari
Sutoro Sutoro
Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae
Jurnal Fitopatologi Indonesia
antagonists, gas chromatography mass spectrometry, rice pathogens, secondary metabolytes
author_facet Yadi Suryadi
I Made Samudra
Tri Puji Priyatno
Dwi Ningsih Susilowati
Puji Lestari
Sutoro Sutoro
author_sort Yadi Suryadi
title Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae
title_short Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae
title_full Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae
title_fullStr Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae
title_full_unstemmed Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae
title_sort aktivitas anticendawan bacillus cereus 11uj terhadap rhizoctonia solani dan pyricularia oryzae
publisher Perhimpunan Fitopatologi Indonesia
series Jurnal Fitopatologi Indonesia
issn 0215-7950
2339-2479
publishDate 2015-04-01
description <p class="Pa8"><span class="A1"><span>Microbial secondary metabolites is an important resource for antifungal development in disease control strategy. The objective of this study was to screen <em>Bacillus cereus </em>11UJ, an antagonistic rhizosphere bacteria for potential secondary metabolite production against rice fungal pathogens, i.e. <em>Rhizoctonia solani </em>and <em>Pyricularia oryzae</em>. The antagonistic effect of crude exract was evaluated using sterile filter paper discs on PDA medium. The ethyl acetate extracts of the bacterium showed a better antifungal activity to <em>P. oryzae </em>than those of <em>R. solani. </em>The inhibitory effect of the filtrate proved the potency of the isolates to produce antifungal. Analysis of pyrolysis gas chromatography-mass spectrometry showed that <em>B. cereus </em>11UJ produces 3 major compounds i.e; 9,19-cyclolanostan-3-ol, acetate, (3.beta.)- (CAS) cycloartanyl acetate (13.14%); 4-(2’,2’-dimethyl-6’-methyliden-1’-cyclohexyliden)-3-methyl-2-butanone (9.72%); and stigmast-5-en-3-ol oleat (9.09%) which suggested to play an important role in the suppression of rice fungal pathogens.</span></span></p> <p class="Default"> </p>
topic antagonists, gas chromatography mass spectrometry, rice pathogens, secondary metabolytes
url http://journal.ipb.ac.id/index.php/jfiti/article/view/9344
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