Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement
The anabolic androgenic steroids (AAS) are the most frequently consumed performance enhancing drugs (PED) in sports. In the anti-doping field, the detection of AAS is carried out by the analysis of the athlete’s urine using methodologies based on liquid/gas chromatography-mass spectrometry. Unfortun...
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doaj-f67137d24671487396bb553bf50d3ed22021-03-27T00:00:45ZengMDPI AGChemosensors2227-90402021-03-019626210.3390/chemosensors9040062Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity MeasurementPilar Martín-Escudero0Jesus A. Muñoz-Guerra1Soledad Vargas García-Tenorio2Ester Serrano-Garde3Ana Belén Soldevilla-Navarro4Nuria Cortes-Carrillo5Mercedes Galindo-Canales6Nayade del Prado7Manuel Fuentes-Ferrer8Cristina Fernández-Pérez9Peter Alexander Behnisch10Abraham Brouwer11Faculty of Medicine, Professional School of Sports Medicine, University Complutense of Madrid, 28040 Madrid, SpainSpanish Agency for Protection of Health in Sport, AEPSAD, 28040 Madrid, SpainDoping Control Laboratory of Madrid, Spanish Agency for Protection of Health in Sport, AEPSAD, 28040 Madrid, SpainDoping Control Laboratory of Madrid, Spanish Agency for Protection of Health in Sport, AEPSAD, 28040 Madrid, SpainDoping Control Laboratory of Madrid, Spanish Agency for Protection of Health in Sport, AEPSAD, 28040 Madrid, SpainDoping Control Laboratory of Madrid, Spanish Agency for Protection of Health in Sport, AEPSAD, 28040 Madrid, SpainFaculty of Medicine, Professional School of Sports Medicine, University Complutense of Madrid, 28040 Madrid, SpainPreventive Medicine Service, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria IdISSC, Hospital Clínico San Carlos, 28040 Madrid, SpainPreventive Medicine Service, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria IdISSC, Hospital Clínico San Carlos, 28040 Madrid, SpainPreventive Medicine Service, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria IdISSC, Hospital Clínico San Carlos, 28040 Madrid, SpainBioDetection Systems BV, Science Park 406, 1098 XH Amsterdam, The NetherlandsBioDetection Systems BV, Science Park 406, 1098 XH Amsterdam, The NetherlandsThe anabolic androgenic steroids (AAS) are the most frequently consumed performance enhancing drugs (PED) in sports. In the anti-doping field, the detection of AAS is carried out by the analysis of the athlete’s urine using methodologies based on liquid/gas chromatography-mass spectrometry. Unfortunately, the detection of unknown compounds is not possible. BDS’s AR CALUX<sup>®</sup> bio detection technology was studied as an indirect method to detect the administration of a single dose of testosterone (T). Twelve T and placebo single dose administered men volunteers underwent a triple-blind crossover clinical trial. The UGT2B17 deletion was present among the volunteers and evenly distributed in heterozygous (ins/del), wild-type homozygous (ins/ins), and mutated homozygous (del/del) groups. A significant statistical difference in terms of bioluminescence was observed after the testosterone (T) administration for the three types of polymorphic groups. The ratio of means between the pre- and post-T administration periods, depending on the type of polymorphism, was in group ins/ins 3.31 (CI. 95%: 2.07–5.29), group ins/del 4.15 (CI 95%: 3.05–5.67), and group del/del 2.89 (CI 95%: 2.42–3.46). The results of the study are very promising, as they may offer us the possibility of designing a detection approach that, based on intra-individual monitoring of androgenic values, in the UGT2B17 deletion type.https://www.mdpi.com/2227-9040/9/4/62dopingUGT2B17 genotypesteroid profiletestosterone administrationsportathlete biological passport |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pilar Martín-Escudero Jesus A. Muñoz-Guerra Soledad Vargas García-Tenorio Ester Serrano-Garde Ana Belén Soldevilla-Navarro Nuria Cortes-Carrillo Mercedes Galindo-Canales Nayade del Prado Manuel Fuentes-Ferrer Cristina Fernández-Pérez Peter Alexander Behnisch Abraham Brouwer |
spellingShingle |
Pilar Martín-Escudero Jesus A. Muñoz-Guerra Soledad Vargas García-Tenorio Ester Serrano-Garde Ana Belén Soldevilla-Navarro Nuria Cortes-Carrillo Mercedes Galindo-Canales Nayade del Prado Manuel Fuentes-Ferrer Cristina Fernández-Pérez Peter Alexander Behnisch Abraham Brouwer Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement Chemosensors doping UGT2B17 genotype steroid profile testosterone administration sport athlete biological passport |
author_facet |
Pilar Martín-Escudero Jesus A. Muñoz-Guerra Soledad Vargas García-Tenorio Ester Serrano-Garde Ana Belén Soldevilla-Navarro Nuria Cortes-Carrillo Mercedes Galindo-Canales Nayade del Prado Manuel Fuentes-Ferrer Cristina Fernández-Pérez Peter Alexander Behnisch Abraham Brouwer |
author_sort |
Pilar Martín-Escudero |
title |
Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement |
title_short |
Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement |
title_full |
Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement |
title_fullStr |
Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement |
title_full_unstemmed |
Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement |
title_sort |
bioanalytical detection of steroid abuse in sports based on the androgenic activity measurement |
publisher |
MDPI AG |
series |
Chemosensors |
issn |
2227-9040 |
publishDate |
2021-03-01 |
description |
The anabolic androgenic steroids (AAS) are the most frequently consumed performance enhancing drugs (PED) in sports. In the anti-doping field, the detection of AAS is carried out by the analysis of the athlete’s urine using methodologies based on liquid/gas chromatography-mass spectrometry. Unfortunately, the detection of unknown compounds is not possible. BDS’s AR CALUX<sup>®</sup> bio detection technology was studied as an indirect method to detect the administration of a single dose of testosterone (T). Twelve T and placebo single dose administered men volunteers underwent a triple-blind crossover clinical trial. The UGT2B17 deletion was present among the volunteers and evenly distributed in heterozygous (ins/del), wild-type homozygous (ins/ins), and mutated homozygous (del/del) groups. A significant statistical difference in terms of bioluminescence was observed after the testosterone (T) administration for the three types of polymorphic groups. The ratio of means between the pre- and post-T administration periods, depending on the type of polymorphism, was in group ins/ins 3.31 (CI. 95%: 2.07–5.29), group ins/del 4.15 (CI 95%: 3.05–5.67), and group del/del 2.89 (CI 95%: 2.42–3.46). The results of the study are very promising, as they may offer us the possibility of designing a detection approach that, based on intra-individual monitoring of androgenic values, in the UGT2B17 deletion type. |
topic |
doping UGT2B17 genotype steroid profile testosterone administration sport athlete biological passport |
url |
https://www.mdpi.com/2227-9040/9/4/62 |
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