An RNA in situ hybridization protocol optimized for monocot tissue

An RNA in situ hybridization protocol optimized for monocot tissue

Summary: RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antise...

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Bibliographic Details
Main Authors: Nora R. Zöllner, Margaret Bezrutczyk, Reinout Laureyns, Hilde Nelissen, Rüdiger Simon, Wolf B. Frommer
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Molecular Biology
In Situ Hybridization
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001052
Description
Summary:Summary: RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense probes for each gene of interest and hybridize them to tissue sections.For complete details on the use and execution of this protocol, please refer to Bezrutczyk et al. (2021).
ISSN:2666-1667

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