Impact of Long-Term Fertilization on Community Structure of Ammonia Oxidizing and Denitrifying Bacteria Based on amoA and nirK Genes in a Rice Paddy from Tai Lake Region, China
Ammonia oxidizing (AOB) and denitrifying bacteria (DNB) play an important role in soil nitrogen transformation in natural and agricultural ecosystems. Effects of long-term fertilization on abundance and community composition of AOB and DNB were studied with targeting ammonia monooxygenase (amoA) and...
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doaj-f679370b197f42138604fc453f0591292021-06-07T06:50:09ZengElsevierJournal of Integrative Agriculture2095-31192014-10-01131022862298Impact of Long-Term Fertilization on Community Structure of Ammonia Oxidizing and Denitrifying Bacteria Based on amoA and nirK Genes in a Rice Paddy from Tai Lake Region, ChinaZhen-jiang JIN0Lian-qing LI1Xiao-yu LIU2Gen-xing PAN3Hussein Qaiser4Yong-zhuo LIU5Institute of Resource, Ecosystem and Environment of Agriculture, Nanjing Agricultural University, Nanjing 210095, P.R.China; Guilin University of Technology, Guilin 541004, P.R.ChinaInstitute of Resource, Ecosystem and Environment of Agriculture, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaInstitute of Resource, Ecosystem and Environment of Agriculture, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaInstitute of Resource, Ecosystem and Environment of Agriculture, Nanjing Agricultural University, Nanjing 210095, P.R.China; Correspondence PAN Gen-xing, Tel: +86-25-84398657Institute of Resource, Ecosystem and Environment of Agriculture, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaInstitute of Resource, Ecosystem and Environment of Agriculture, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaAmmonia oxidizing (AOB) and denitrifying bacteria (DNB) play an important role in soil nitrogen transformation in natural and agricultural ecosystems. Effects of long-term fertilization on abundance and community composition of AOB and DNB were studied with targeting ammonia monooxygenase (amoA) and nitrite reductase (nirK) genes using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and real-time PCR, respectively. A field trial with different fertilization treatments in a rice paddy from Tai Lake region, centre East China was used in this study, including no fertilizer application (NF), balanced chemical fertilizers (CF), combined organic/inorganic fertilizer of balanced chemical fertilizers plus pig manure (CFM), and plus rice straw return (CFS). The abundances and richnesses of amoA and nirK were increased in CF, CFM and CFS compared to NF. Principle component analysis of DGGE profiles showed significant difference in nirK and amoA genes composition between organic amended (CFS and CFM) and the non-organic amended (CF and NF) plots. Number of amoA copies was significantly positively correlated with normalized soil nutrient richness (NSNR) of soil organic carbon (SOC) and total nitrogen (T-N), and that of nirK copies was with NSNR of SOC, T-N plus total phosphorus. Moreover, nitrification potential showed a positive correlation with SOC content, while a significantly lower denitrification potential was found under CFM compared to under CFS. Therefore, SOC accumulation accompanied with soil nutrient richness under long-term balanced and organic/inorganic combined fertilization promoted abundance and diversity of AOB and DNB in the rice paddy.http://www.sciencedirect.com/science/article/pii/S209531191460784Xlong-term fertilizationammonia oxidizing bacteriadenitrifying bacteriaabundancerice paddy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhen-jiang JIN Lian-qing LI Xiao-yu LIU Gen-xing PAN Hussein Qaiser Yong-zhuo LIU |
spellingShingle |
Zhen-jiang JIN Lian-qing LI Xiao-yu LIU Gen-xing PAN Hussein Qaiser Yong-zhuo LIU Impact of Long-Term Fertilization on Community Structure of Ammonia Oxidizing and Denitrifying Bacteria Based on amoA and nirK Genes in a Rice Paddy from Tai Lake Region, China Journal of Integrative Agriculture long-term fertilization ammonia oxidizing bacteria denitrifying bacteria abundance rice paddy |
author_facet |
Zhen-jiang JIN Lian-qing LI Xiao-yu LIU Gen-xing PAN Hussein Qaiser Yong-zhuo LIU |
author_sort |
Zhen-jiang JIN |
title |
Impact of Long-Term Fertilization on Community Structure of Ammonia Oxidizing and Denitrifying Bacteria Based on amoA and nirK Genes in a Rice Paddy from Tai Lake Region, China |
title_short |
Impact of Long-Term Fertilization on Community Structure of Ammonia Oxidizing and Denitrifying Bacteria Based on amoA and nirK Genes in a Rice Paddy from Tai Lake Region, China |
title_full |
Impact of Long-Term Fertilization on Community Structure of Ammonia Oxidizing and Denitrifying Bacteria Based on amoA and nirK Genes in a Rice Paddy from Tai Lake Region, China |
title_fullStr |
Impact of Long-Term Fertilization on Community Structure of Ammonia Oxidizing and Denitrifying Bacteria Based on amoA and nirK Genes in a Rice Paddy from Tai Lake Region, China |
title_full_unstemmed |
Impact of Long-Term Fertilization on Community Structure of Ammonia Oxidizing and Denitrifying Bacteria Based on amoA and nirK Genes in a Rice Paddy from Tai Lake Region, China |
title_sort |
impact of long-term fertilization on community structure of ammonia oxidizing and denitrifying bacteria based on amoa and nirk genes in a rice paddy from tai lake region, china |
publisher |
Elsevier |
series |
Journal of Integrative Agriculture |
issn |
2095-3119 |
publishDate |
2014-10-01 |
description |
Ammonia oxidizing (AOB) and denitrifying bacteria (DNB) play an important role in soil nitrogen transformation in natural and agricultural ecosystems. Effects of long-term fertilization on abundance and community composition of AOB and DNB were studied with targeting ammonia monooxygenase (amoA) and nitrite reductase (nirK) genes using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and real-time PCR, respectively. A field trial with different fertilization treatments in a rice paddy from Tai Lake region, centre East China was used in this study, including no fertilizer application (NF), balanced chemical fertilizers (CF), combined organic/inorganic fertilizer of balanced chemical fertilizers plus pig manure (CFM), and plus rice straw return (CFS). The abundances and richnesses of amoA and nirK were increased in CF, CFM and CFS compared to NF. Principle component analysis of DGGE profiles showed significant difference in nirK and amoA genes composition between organic amended (CFS and CFM) and the non-organic amended (CF and NF) plots. Number of amoA copies was significantly positively correlated with normalized soil nutrient richness (NSNR) of soil organic carbon (SOC) and total nitrogen (T-N), and that of nirK copies was with NSNR of SOC, T-N plus total phosphorus. Moreover, nitrification potential showed a positive correlation with SOC content, while a significantly lower denitrification potential was found under CFM compared to under CFS. Therefore, SOC accumulation accompanied with soil nutrient richness under long-term balanced and organic/inorganic combined fertilization promoted abundance and diversity of AOB and DNB in the rice paddy. |
topic |
long-term fertilization ammonia oxidizing bacteria denitrifying bacteria abundance rice paddy |
url |
http://www.sciencedirect.com/science/article/pii/S209531191460784X |
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