Recycling endosomes associate with Golgi stacks in sea urchin embryos
The trans-Golgi network (TGN) and recycling endosome (RE) have been recognized as sorting centers, the former for newly synthesized and the latter for endocytosed proteins. However, recent findings have revealed that TGN also receives endocytosed materials and RE accepts newly synthesized proteins d...
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Online Access: | http://dx.doi.org/10.1080/19420889.2020.1761069 |
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doaj-f67b1b313a30437c815c255e297913c62021-02-08T14:09:14ZengTaylor & Francis GroupCommunicative & Integrative Biology1942-08892020-01-01131596210.1080/19420889.2020.17610691761069Recycling endosomes associate with Golgi stacks in sea urchin embryosSyara Fujii0Tatsuya Tago1Naoaki Sakamoto2Takashi Yamamoto3Takunori Satoh4Akiko K. Satoh5Hiroshima UniversityHiroshima UniversityHiroshima UniversityHiroshima UniversityHiroshima UniversityHiroshima UniversityThe trans-Golgi network (TGN) and recycling endosome (RE) have been recognized as sorting centers, the former for newly synthesized and the latter for endocytosed proteins. However, recent findings have revealed that TGN also receives endocytosed materials and RE accepts newly synthesized proteins destined to the plasma membrane. Recently, we reported that in both Drosophila and microtubule-disrupted HeLa cells, REs are associated with the trans-side of Golgi stacks. REs are highly dynamic: their separation from and association with Golgi stacks are often observed. Importantly, a newly synthesized cargo, glycosylphosphatidylinositol-anchored-GFP was found to be concentrated in Golgi-associated REs (GA-REs), while another cargo VSVG-GFP was excluded from GA-REs before post-Golgi trafficking to the plasma membrane. This suggested that the sorting of cargos takes place at the interface of Golgi stacks and GA-REs. In this study, we demonstrated that REs could associate with Golgi stacks in sea urchin embryos, further indicating that the association of REs with Golgi stacks is a well-conserved phenomenon in the animal kingdom.http://dx.doi.org/10.1080/19420889.2020.1761069recycling endosometrans-golgi networksea urchingolgi stackembryomrna injection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Syara Fujii Tatsuya Tago Naoaki Sakamoto Takashi Yamamoto Takunori Satoh Akiko K. Satoh |
spellingShingle |
Syara Fujii Tatsuya Tago Naoaki Sakamoto Takashi Yamamoto Takunori Satoh Akiko K. Satoh Recycling endosomes associate with Golgi stacks in sea urchin embryos Communicative & Integrative Biology recycling endosome trans-golgi network sea urchin golgi stack embryo mrna injection |
author_facet |
Syara Fujii Tatsuya Tago Naoaki Sakamoto Takashi Yamamoto Takunori Satoh Akiko K. Satoh |
author_sort |
Syara Fujii |
title |
Recycling endosomes associate with Golgi stacks in sea urchin embryos |
title_short |
Recycling endosomes associate with Golgi stacks in sea urchin embryos |
title_full |
Recycling endosomes associate with Golgi stacks in sea urchin embryos |
title_fullStr |
Recycling endosomes associate with Golgi stacks in sea urchin embryos |
title_full_unstemmed |
Recycling endosomes associate with Golgi stacks in sea urchin embryos |
title_sort |
recycling endosomes associate with golgi stacks in sea urchin embryos |
publisher |
Taylor & Francis Group |
series |
Communicative & Integrative Biology |
issn |
1942-0889 |
publishDate |
2020-01-01 |
description |
The trans-Golgi network (TGN) and recycling endosome (RE) have been recognized as sorting centers, the former for newly synthesized and the latter for endocytosed proteins. However, recent findings have revealed that TGN also receives endocytosed materials and RE accepts newly synthesized proteins destined to the plasma membrane. Recently, we reported that in both Drosophila and microtubule-disrupted HeLa cells, REs are associated with the trans-side of Golgi stacks. REs are highly dynamic: their separation from and association with Golgi stacks are often observed. Importantly, a newly synthesized cargo, glycosylphosphatidylinositol-anchored-GFP was found to be concentrated in Golgi-associated REs (GA-REs), while another cargo VSVG-GFP was excluded from GA-REs before post-Golgi trafficking to the plasma membrane. This suggested that the sorting of cargos takes place at the interface of Golgi stacks and GA-REs. In this study, we demonstrated that REs could associate with Golgi stacks in sea urchin embryos, further indicating that the association of REs with Golgi stacks is a well-conserved phenomenon in the animal kingdom. |
topic |
recycling endosome trans-golgi network sea urchin golgi stack embryo mrna injection |
url |
http://dx.doi.org/10.1080/19420889.2020.1761069 |
work_keys_str_mv |
AT syarafujii recyclingendosomesassociatewithgolgistacksinseaurchinembryos AT tatsuyatago recyclingendosomesassociatewithgolgistacksinseaurchinembryos AT naoakisakamoto recyclingendosomesassociatewithgolgistacksinseaurchinembryos AT takashiyamamoto recyclingendosomesassociatewithgolgistacksinseaurchinembryos AT takunorisatoh recyclingendosomesassociatewithgolgistacksinseaurchinembryos AT akikoksatoh recyclingendosomesassociatewithgolgistacksinseaurchinembryos |
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1724279996964405248 |