Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit
Lasting changes in gene expression are critical for the formation of long-term memories (LTMs), depending on the conserved CrebB transcriptional activator. While requirement of distinct neurons in defined circuits for different learning and memory phases have been studied in detail, only little is k...
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doaj-f67c54754c504fa2a084bd480a506be32021-05-05T16:14:01ZengeLife Sciences Publications LtdeLife2050-084X2018-10-01710.7554/eLife.39196Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuitYves F Widmer0https://orcid.org/0000-0002-8880-1392Cornelia Fritsch1Magali M Jungo2Silvia Almeida3Boris Egger4Simon G Sprecher5https://orcid.org/0000-0001-9060-3750Department of Biology, University of Fribourg, Fribourg, SwitzerlandDepartment of Biology, University of Fribourg, Fribourg, SwitzerlandDepartment of Biology, University of Fribourg, Fribourg, SwitzerlandDepartment of Biology, University of Fribourg, Fribourg, SwitzerlandDepartment of Biology, University of Fribourg, Fribourg, SwitzerlandDepartment of Biology, University of Fribourg, Fribourg, SwitzerlandLasting changes in gene expression are critical for the formation of long-term memories (LTMs), depending on the conserved CrebB transcriptional activator. While requirement of distinct neurons in defined circuits for different learning and memory phases have been studied in detail, only little is known regarding the gene regulatory changes that occur within these neurons. We here use the fruit fly as powerful model system to study the neural circuits of CrebB-dependent appetitive olfactory LTM. We edited the CrebB locus to create a GFP-tagged CrebB conditional knockout allele, allowing us to generate mutant, post-mitotic neurons with high spatial and temporal precision. Investigating CrebB-dependence within the mushroom body (MB) circuit we show that MB α/β and α’/β’ neurons as well as MBON α3, but not in dopaminergic neurons require CrebB for LTM. Thus, transcriptional memory traces occur in different neurons within the same neural circuit.https://elifesciences.org/articles/39196long-term memoryCreblearning |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yves F Widmer Cornelia Fritsch Magali M Jungo Silvia Almeida Boris Egger Simon G Sprecher |
spellingShingle |
Yves F Widmer Cornelia Fritsch Magali M Jungo Silvia Almeida Boris Egger Simon G Sprecher Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit eLife long-term memory Creb learning |
author_facet |
Yves F Widmer Cornelia Fritsch Magali M Jungo Silvia Almeida Boris Egger Simon G Sprecher |
author_sort |
Yves F Widmer |
title |
Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit |
title_short |
Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit |
title_full |
Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit |
title_fullStr |
Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit |
title_full_unstemmed |
Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit |
title_sort |
multiple neurons encode crebb dependent appetitive long-term memory in the mushroom body circuit |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2018-10-01 |
description |
Lasting changes in gene expression are critical for the formation of long-term memories (LTMs), depending on the conserved CrebB transcriptional activator. While requirement of distinct neurons in defined circuits for different learning and memory phases have been studied in detail, only little is known regarding the gene regulatory changes that occur within these neurons. We here use the fruit fly as powerful model system to study the neural circuits of CrebB-dependent appetitive olfactory LTM. We edited the CrebB locus to create a GFP-tagged CrebB conditional knockout allele, allowing us to generate mutant, post-mitotic neurons with high spatial and temporal precision. Investigating CrebB-dependence within the mushroom body (MB) circuit we show that MB α/β and α’/β’ neurons as well as MBON α3, but not in dopaminergic neurons require CrebB for LTM. Thus, transcriptional memory traces occur in different neurons within the same neural circuit. |
topic |
long-term memory Creb learning |
url |
https://elifesciences.org/articles/39196 |
work_keys_str_mv |
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