Summary: | Apple mosaic virus (ApMV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting
virus (ASPV), and Apple stem grooving virus (ASGV), and together with Plum pox virus
(PPV) are regularly monitored worldwide in fruit trees. These viruses are usually
routinely tested using a serological method enzyme linked immunosorbent assays (ELISA). However, the suitability of the ELISA method for virus testing throughout the
entire year is limited by an inconsistent reliability of virus detections in plant tissues
during seasons. Our work focused on suitability of plant tissues sampled during the
wintertime for the detection of the apple viruses and PPV using ELISA. Shoots of
infected apple and plum trees were sampled in four terms in January, and either
dormant buds or sprouting buds were used for the testing. We found that ACLSV,
ApMV, and ASPV could be reliably detected in buds after 7 days of sprouting.
However, 14 days of sprouting was necessary for ASGV. Ideally, sampling should be
carried out in the second half of January. PPV was reliably detected during the entire
January from dormant buds without any need of forced sprouting.
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