Toxicopathological interaction of lead and cadmium and amelioration with N-Acetyl-L-Cysteine

Aim: To investigate the nephrotoxicity induced by the combined exposure of lead (Pb) and cadmium (Cd) and its ameliorationwith N-acetyl-L-cysteine (NAC).Materials and Methods: To evaluate the nephrotoxicity of Pb and Cd, the present study was taken up with a total of 48 malewistar rats, divided unif...

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Main Authors: B. Anilkumar, A. Gopala Reddy, A. Anand Kumar, G. Ambica, C. Haritha
Format: Article
Language:English
Published: Veterinary World 2013-09-01
Series:Veterinary World
Subjects:
rat
Online Access:http://www.veterinaryworld.org/Vol.6/Oct-2013/26.pdf
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spelling doaj-f68c337199864183a4ff975fea8f7c3e2021-08-02T19:19:35ZengVeterinary WorldVeterinary World0972-89882231-09162013-09-0161082382710.14202/vetworld.2013.823-827Toxicopathological interaction of lead and cadmium and amelioration with N-Acetyl-L-CysteineB. AnilkumarA. Gopala ReddyA. Anand KumarG. AmbicaC. HarithaAim: To investigate the nephrotoxicity induced by the combined exposure of lead (Pb) and cadmium (Cd) and its ameliorationwith N-acetyl-L-cysteine (NAC).Materials and Methods: To evaluate the nephrotoxicity of Pb and Cd, the present study was taken up with a total of 48 malewistar rats, divided uniformly into eight groups each with 6 rats. Groups I and II were kept as untreated control and NACcontrol, respectively. Groups III, IV and V served as toxic control groups viz., Pb, Cd and combination of Pb+ Cd @ 1000, 300and 1000+300 ppm, respectively. Whereas groups VI, VII and VIII were co exposed with NAC and served as therapeuticgroups (Pb, Cd and combination of Pb+ Cd @ 1000, 300 and 1000+300 ppm, respectively and NAC @ 300mg/ kg). Theexperiment was carried out for 3 months and kidney tissues were collected for histopathological and electron microscopicstudy at the end of the experiment.Results: Histopathological examination of kidney tissue revealed marked alterations and electron microscopic observationsalso showed marked ultrastructural changes and these changes were more pronounced in Pb and Cd co exposure group (group5) than the Pb and Cd alone exposed groups (groups 3 and 4), respectively. We also observed a significant (p<0.05) elevation inthe serum creatinine levels. Use of the NAC counteracted the all toxic effects by chelating these metals, thereby reducing theiraccumulation and NAC also demonstrated its antioxidant properties, as judged by a significant reduction in the serumcreatinine levels.Conclusion: We conclude that the co-existence of these metals showed a positive pharmacodynamic interaction andsignificant improvement was noticed after NAC therapy.http://www.veterinaryworld.org/Vol.6/Oct-2013/26.pdfcadmiumleadN-Acetyl-L-Cysteinerat
collection DOAJ
language English
format Article
sources DOAJ
author B. Anilkumar
A. Gopala Reddy
A. Anand Kumar
G. Ambica
C. Haritha
spellingShingle B. Anilkumar
A. Gopala Reddy
A. Anand Kumar
G. Ambica
C. Haritha
Toxicopathological interaction of lead and cadmium and amelioration with N-Acetyl-L-Cysteine
Veterinary World
cadmium
lead
N-Acetyl-L-Cysteine
rat
author_facet B. Anilkumar
A. Gopala Reddy
A. Anand Kumar
G. Ambica
C. Haritha
author_sort B. Anilkumar
title Toxicopathological interaction of lead and cadmium and amelioration with N-Acetyl-L-Cysteine
title_short Toxicopathological interaction of lead and cadmium and amelioration with N-Acetyl-L-Cysteine
title_full Toxicopathological interaction of lead and cadmium and amelioration with N-Acetyl-L-Cysteine
title_fullStr Toxicopathological interaction of lead and cadmium and amelioration with N-Acetyl-L-Cysteine
title_full_unstemmed Toxicopathological interaction of lead and cadmium and amelioration with N-Acetyl-L-Cysteine
title_sort toxicopathological interaction of lead and cadmium and amelioration with n-acetyl-l-cysteine
publisher Veterinary World
series Veterinary World
issn 0972-8988
2231-0916
publishDate 2013-09-01
description Aim: To investigate the nephrotoxicity induced by the combined exposure of lead (Pb) and cadmium (Cd) and its ameliorationwith N-acetyl-L-cysteine (NAC).Materials and Methods: To evaluate the nephrotoxicity of Pb and Cd, the present study was taken up with a total of 48 malewistar rats, divided uniformly into eight groups each with 6 rats. Groups I and II were kept as untreated control and NACcontrol, respectively. Groups III, IV and V served as toxic control groups viz., Pb, Cd and combination of Pb+ Cd @ 1000, 300and 1000+300 ppm, respectively. Whereas groups VI, VII and VIII were co exposed with NAC and served as therapeuticgroups (Pb, Cd and combination of Pb+ Cd @ 1000, 300 and 1000+300 ppm, respectively and NAC @ 300mg/ kg). Theexperiment was carried out for 3 months and kidney tissues were collected for histopathological and electron microscopicstudy at the end of the experiment.Results: Histopathological examination of kidney tissue revealed marked alterations and electron microscopic observationsalso showed marked ultrastructural changes and these changes were more pronounced in Pb and Cd co exposure group (group5) than the Pb and Cd alone exposed groups (groups 3 and 4), respectively. We also observed a significant (p<0.05) elevation inthe serum creatinine levels. Use of the NAC counteracted the all toxic effects by chelating these metals, thereby reducing theiraccumulation and NAC also demonstrated its antioxidant properties, as judged by a significant reduction in the serumcreatinine levels.Conclusion: We conclude that the co-existence of these metals showed a positive pharmacodynamic interaction andsignificant improvement was noticed after NAC therapy.
topic cadmium
lead
N-Acetyl-L-Cysteine
rat
url http://www.veterinaryworld.org/Vol.6/Oct-2013/26.pdf
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AT agopalareddy toxicopathologicalinteractionofleadandcadmiumandameliorationwithnacetyllcysteine
AT aanandkumar toxicopathologicalinteractionofleadandcadmiumandameliorationwithnacetyllcysteine
AT gambica toxicopathologicalinteractionofleadandcadmiumandameliorationwithnacetyllcysteine
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