Contamination of Chinese Cabbage Soil with Plasmodiophora brassicae
This research was performed to establish basic technology for Chinese cabbage clubroot chemical control by investigating the soil contamination of Plasmodiophora brassicae in major producing regions of fall Chinese cabbage. PCR primers were developed to detect P. brassicae, a causal agent of Chine...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2013-09-01
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| Series: | Research in Plant Disease |
| Subjects: | |
| Online Access: | http://www.online-rpd.org/journal/view.html?uid=721&sort=book_Seq&scale=&key=year&keyword=&s_v=19&s_n=3&pn=vol&year=2013&vmd=Full |
| Summary: | This research was performed to establish basic technology for Chinese cabbage clubroot chemical control by
investigating the soil contamination of Plasmodiophora brassicae in major producing regions of fall Chinese
cabbage. PCR primers were developed to detect P. brassicae, a causal agent of Chinese cabbage club-root that
generally occurs in Cruciferae family. A primer set, PbbtgF761 and PbbtgR961, specifically amplified a
245 bp fragment from P. brassicae only. At places well known for fall Chinese cabbage, 10 out of 33 in
Haenam-gun, 5 out of 13 in Yeongam-gun and Yeonggwang-gun, 1 out of 6 in Gochang-gun, 2 out of 12 in
Hongseong-gun, and 5 out of 17 in Dangjin-si resulted positive for P. brassicae contamination. The results
show that the soil contamination rate of P. brassicae was 30.3% in Haenam-gun, 38.5% in Yeongam-gun and
Yeonggwang-gun, 16.7% in Gochang-gun, 16.7% in Hongseong-gun, and 29.4% in Dangjin-si. The six places
where Chinese cabbage clubroot was visible by naked eye were 100% confirmed by the PCR test of the P.
brassicae contaminated soil. Thus, simple PCR test may be utilized as an index to decide on chemical control
of P. brassicae. |
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| ISSN: | 1598-2262 2233-9191 |