Empowering Shotgun Mass Spectrometry with 2DE: A HepG2 Study

• Main Authors: Olga Kiseleva, Elena Ponomarenko, Ekaterina Poverennaya
• Format: Article
• Language: English
• Published: MDPI AG 2020-05-01
• Series: International Journal of Molecular Sciences
• Subjects: proteome; transcriptome; proteoforms; alternative splicing; single amino acid polymorphisms; post-translational modifications
• Online Access: https://www.mdpi.com/1422-0067/21/11/3813

One of the major goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to catalog and annotate a myriad of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve a detailed and personalized understanding into proteomes, we suggest using a customized RNA-seq library of potential proteoforms, which includes aberrant variants specific to certain biological samples. Two-dimensional electrophoresis coupled with high-performance liquid chromatography allowed us to downgrade the difficulty of biological mixing following shotgun mass spectrometry. To benchmark the proposed pipeline, we examined heterogeneity of the HepG2 hepatoblastoma cell line proteome. Data are available via ProteomeXchange with identifier PXD018450.