Ionic Nanocomplexes of Hyaluronic Acid and Polyarginine to Form Solid Materials: A Green Methodology to Obtain Sponges with Biomedical Potential
We report on the design, development, characterization, and a preliminary cellular evaluation of a novel solid material. This material is composed of low-molecular-weight hyaluronic acid (LMWHA) and polyarginine (PArg), which generate aqueous ionic nanocomplexes (INC) that are then freeze-dried to c...
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doaj-f6ac531a1d4d4e15b236df92b3bd04a42020-11-25T01:49:48ZengMDPI AGNanomaterials2079-49912019-06-019794410.3390/nano9070944nano9070944Ionic Nanocomplexes of Hyaluronic Acid and Polyarginine to Form Solid Materials: A Green Methodology to Obtain Sponges with Biomedical PotentialMaría Gabriela Villamizar-Sarmiento0Ignacio Moreno-Villoslada1Samuel Martínez2Annesi Giacaman3Victor Miranda4Alejandra Vidal5Sandra L. Orellana6Miguel Concha7Francisca Pavicic8Judit G. Lisoni9Lisette Leyton10Felipe A. Oyarzun-Ampuero11Advanced Center of Chronic Diseases (ACCDiS), Universidad de Chile, Santos Dumont 964, Independencia, Santiago 8380494, ChileInstituto de Ciencias Químicas, Facultad de Ciencias, Universidad Austral de Chile, Isla Teja, Casilla 567, Valdivia 5090000, ChileAdvanced Center of Chronic Diseases (ACCDiS), Universidad de Chile, Santos Dumont 964, Independencia, Santiago 8380494, ChileInstituto de Anatomía, Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia 5090000, ChileDepartamento de Ciencias y Tecnología Farmacéuticas, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santos Dumont 964, Independencia, Santiago 8380494, ChileInstituto de Anatomía, Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia 5090000, ChileInstituto de Ciencias Químicas, Facultad de Ciencias, Universidad Austral de Chile, Isla Teja, Casilla 567, Valdivia 5090000, ChileInstituto de Anatomía, Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia 5090000, ChileInstituto de Anatomía, Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia 5090000, ChileNM MultiMat, Instituto de Ciencias Físicas y Matemáticas, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 5090000, ChileAdvanced Center of Chronic Diseases (ACCDiS), Universidad de Chile, Santos Dumont 964, Independencia, Santiago 8380494, ChileAdvanced Center of Chronic Diseases (ACCDiS), Universidad de Chile, Santos Dumont 964, Independencia, Santiago 8380494, ChileWe report on the design, development, characterization, and a preliminary cellular evaluation of a novel solid material. This material is composed of low-molecular-weight hyaluronic acid (LMWHA) and polyarginine (PArg), which generate aqueous ionic nanocomplexes (INC) that are then freeze-dried to create the final product. Different ratios of LMWHA/PArg were selected to elaborate INC, the size and zeta potential of which ranged from 100 to 200 nm and +25 to −43 mV, respectively. Turbidimetry and nanoparticle concentration analyses demonstrated the high capacity of the INC to interact with increasing concentrations of LMWHA, improving the yield of production of the nanostructures. Interestingly, once the selected formulations of INC were freeze-dried, only those comprising a larger excess of LMWHA could form reproducible sponge formulations, as seen with the naked eye. This optical behavior was consistent with the scanning transmission electron microscopy (STEM) images, which showed a tendency of the particles to agglomerate when an excess of LMWHA was present. Mechanical characterization evidenced low stiffness in the materials, attributed to the low density and high porosity. A preliminary cellular evaluation in a fibroblast cell line (RMF-EG) evidenced the concentration range where swollen formulations did not affect cell proliferation (93−464 µM) at 24, 48, or 72 h. Considering that the reproducible sponge formulations were elaborated following inexpensive and non-contaminant methods and comprised bioactive components, we postulate them with potential for biomedical purposes. Additionally, this systematic study provides important information to design reproducible porous solid materials using ionic nanocomplexes.https://www.mdpi.com/2079-4991/9/7/944self-assemblyspongesionic nanocomplexespolyargininehyaluronic acidcell proliferation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
María Gabriela Villamizar-Sarmiento Ignacio Moreno-Villoslada Samuel Martínez Annesi Giacaman Victor Miranda Alejandra Vidal Sandra L. Orellana Miguel Concha Francisca Pavicic Judit G. Lisoni Lisette Leyton Felipe A. Oyarzun-Ampuero |
spellingShingle |
María Gabriela Villamizar-Sarmiento Ignacio Moreno-Villoslada Samuel Martínez Annesi Giacaman Victor Miranda Alejandra Vidal Sandra L. Orellana Miguel Concha Francisca Pavicic Judit G. Lisoni Lisette Leyton Felipe A. Oyarzun-Ampuero Ionic Nanocomplexes of Hyaluronic Acid and Polyarginine to Form Solid Materials: A Green Methodology to Obtain Sponges with Biomedical Potential Nanomaterials self-assembly sponges ionic nanocomplexes polyarginine hyaluronic acid cell proliferation |
author_facet |
María Gabriela Villamizar-Sarmiento Ignacio Moreno-Villoslada Samuel Martínez Annesi Giacaman Victor Miranda Alejandra Vidal Sandra L. Orellana Miguel Concha Francisca Pavicic Judit G. Lisoni Lisette Leyton Felipe A. Oyarzun-Ampuero |
author_sort |
María Gabriela Villamizar-Sarmiento |
title |
Ionic Nanocomplexes of Hyaluronic Acid and Polyarginine to Form Solid Materials: A Green Methodology to Obtain Sponges with Biomedical Potential |
title_short |
Ionic Nanocomplexes of Hyaluronic Acid and Polyarginine to Form Solid Materials: A Green Methodology to Obtain Sponges with Biomedical Potential |
title_full |
Ionic Nanocomplexes of Hyaluronic Acid and Polyarginine to Form Solid Materials: A Green Methodology to Obtain Sponges with Biomedical Potential |
title_fullStr |
Ionic Nanocomplexes of Hyaluronic Acid and Polyarginine to Form Solid Materials: A Green Methodology to Obtain Sponges with Biomedical Potential |
title_full_unstemmed |
Ionic Nanocomplexes of Hyaluronic Acid and Polyarginine to Form Solid Materials: A Green Methodology to Obtain Sponges with Biomedical Potential |
title_sort |
ionic nanocomplexes of hyaluronic acid and polyarginine to form solid materials: a green methodology to obtain sponges with biomedical potential |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2019-06-01 |
description |
We report on the design, development, characterization, and a preliminary cellular evaluation of a novel solid material. This material is composed of low-molecular-weight hyaluronic acid (LMWHA) and polyarginine (PArg), which generate aqueous ionic nanocomplexes (INC) that are then freeze-dried to create the final product. Different ratios of LMWHA/PArg were selected to elaborate INC, the size and zeta potential of which ranged from 100 to 200 nm and +25 to −43 mV, respectively. Turbidimetry and nanoparticle concentration analyses demonstrated the high capacity of the INC to interact with increasing concentrations of LMWHA, improving the yield of production of the nanostructures. Interestingly, once the selected formulations of INC were freeze-dried, only those comprising a larger excess of LMWHA could form reproducible sponge formulations, as seen with the naked eye. This optical behavior was consistent with the scanning transmission electron microscopy (STEM) images, which showed a tendency of the particles to agglomerate when an excess of LMWHA was present. Mechanical characterization evidenced low stiffness in the materials, attributed to the low density and high porosity. A preliminary cellular evaluation in a fibroblast cell line (RMF-EG) evidenced the concentration range where swollen formulations did not affect cell proliferation (93−464 µM) at 24, 48, or 72 h. Considering that the reproducible sponge formulations were elaborated following inexpensive and non-contaminant methods and comprised bioactive components, we postulate them with potential for biomedical purposes. Additionally, this systematic study provides important information to design reproducible porous solid materials using ionic nanocomplexes. |
topic |
self-assembly sponges ionic nanocomplexes polyarginine hyaluronic acid cell proliferation |
url |
https://www.mdpi.com/2079-4991/9/7/944 |
work_keys_str_mv |
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