Environmental Risk Assessment of Living Modified Microorganisms (LMM) on the Indigenous Microbial Community
Recent advance of biotechnology enabled development of various living modified microorganisms (LMMs) uses in the field of environmental remediation, food industry, biopesticide, and so on. Consequently, such LMMs have the potential to be released into the natural environment, either intentionally or...
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doaj-f6b06bbc412b4a0e974d8763a13cf5852020-11-25T02:50:48ZengMDPI AGSustainability2071-10502020-07-01125566556610.3390/su12145566Environmental Risk Assessment of Living Modified Microorganisms (LMM) on the Indigenous Microbial CommunityHyosun Lee0Dong-Uk Kim1Jigwan Son2Seong-Bo Kim3Jong-Ok Ka4Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Biological Science, College of Science and Engineering, Sangji University, Wonju-si 26339, KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaBio-Living Engineering Major, Global Leaders College, Yonsei University, Seoul 03722, KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaRecent advance of biotechnology enabled development of various living modified microorganisms (LMMs) uses in the field of environmental remediation, food industry, biopesticide, and so on. Consequently, such LMMs have the potential to be released into the natural environment, either intentionally or unintentionally, or exposed to the natural ecosystem during the applications. To investigate the unintended effects of LMMs on soil microorganism populations and communities, microcosm study was conducted using the recombinant microorganism, <i>Corynebacterium glutamicum</i> SEM002 carrying the D-psicose-3-epimerase from <i>Agrobacterium tumefaciens</i> as a model LMM. In addition, potential gene transfer from the LMMs into the soil environment in the microcosm was examined. As a result, small differences in LMMs were observed in populations of soil microorganism such as total bacteria, kanamycin-resistant bacteria, total fungi and total actinomycete. Also, more than 93% of the kanamycin resistance gene from the LMMs was degraded in the microcosm during the 90 days. On the basis of the experimental results, the LMMs showed no distinct impact on soil microorganism populations and communities.https://www.mdpi.com/2071-1050/12/14/5566environmental risk assessmentmicrobial communityliving modified microorganisms<i>Corynebacterium glutamicum</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hyosun Lee Dong-Uk Kim Jigwan Son Seong-Bo Kim Jong-Ok Ka |
spellingShingle |
Hyosun Lee Dong-Uk Kim Jigwan Son Seong-Bo Kim Jong-Ok Ka Environmental Risk Assessment of Living Modified Microorganisms (LMM) on the Indigenous Microbial Community Sustainability environmental risk assessment microbial community living modified microorganisms <i>Corynebacterium glutamicum</i> |
author_facet |
Hyosun Lee Dong-Uk Kim Jigwan Son Seong-Bo Kim Jong-Ok Ka |
author_sort |
Hyosun Lee |
title |
Environmental Risk Assessment of Living Modified Microorganisms (LMM) on the Indigenous Microbial Community |
title_short |
Environmental Risk Assessment of Living Modified Microorganisms (LMM) on the Indigenous Microbial Community |
title_full |
Environmental Risk Assessment of Living Modified Microorganisms (LMM) on the Indigenous Microbial Community |
title_fullStr |
Environmental Risk Assessment of Living Modified Microorganisms (LMM) on the Indigenous Microbial Community |
title_full_unstemmed |
Environmental Risk Assessment of Living Modified Microorganisms (LMM) on the Indigenous Microbial Community |
title_sort |
environmental risk assessment of living modified microorganisms (lmm) on the indigenous microbial community |
publisher |
MDPI AG |
series |
Sustainability |
issn |
2071-1050 |
publishDate |
2020-07-01 |
description |
Recent advance of biotechnology enabled development of various living modified microorganisms (LMMs) uses in the field of environmental remediation, food industry, biopesticide, and so on. Consequently, such LMMs have the potential to be released into the natural environment, either intentionally or unintentionally, or exposed to the natural ecosystem during the applications. To investigate the unintended effects of LMMs on soil microorganism populations and communities, microcosm study was conducted using the recombinant microorganism, <i>Corynebacterium glutamicum</i> SEM002 carrying the D-psicose-3-epimerase from <i>Agrobacterium tumefaciens</i> as a model LMM. In addition, potential gene transfer from the LMMs into the soil environment in the microcosm was examined. As a result, small differences in LMMs were observed in populations of soil microorganism such as total bacteria, kanamycin-resistant bacteria, total fungi and total actinomycete. Also, more than 93% of the kanamycin resistance gene from the LMMs was degraded in the microcosm during the 90 days. On the basis of the experimental results, the LMMs showed no distinct impact on soil microorganism populations and communities. |
topic |
environmental risk assessment microbial community living modified microorganisms <i>Corynebacterium glutamicum</i> |
url |
https://www.mdpi.com/2071-1050/12/14/5566 |
work_keys_str_mv |
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