Characterization of Novel Microsatellite Loci for Primula poissonii (Primulaceae) Using High-Throughput Sequencing Technology
Primula poissonii (Primulaceae) is a perennial herb, widely distributed in the Hengduan Mountain region of Southwest China. In this study, Roche 454 pyrosequencing was used to isolate microsatellite markers. A total of 4528 unique sequences were identified from 68,070 unique reads. Of these, eighty-...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2016-05-01
|
Series: | Molecules |
Subjects: | |
Online Access: | http://www.mdpi.com/1420-3049/21/5/536 |
id |
doaj-f6bea97590f344839602a4e3cef5c2eb |
---|---|
record_format |
Article |
spelling |
doaj-f6bea97590f344839602a4e3cef5c2eb2020-11-24T22:18:53ZengMDPI AGMolecules1420-30492016-05-0121553610.3390/molecules21050536molecules21050536Characterization of Novel Microsatellite Loci for Primula poissonii (Primulaceae) Using High-Throughput Sequencing TechnologyYun-Jiao Liu0Cai-Yun Zhang1Gang Hao2Xue-Jun Ge3Hai-Fei Yan4Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaCollege of Life Sciences, South China Agricultural University, Guangzhou 510642, ChinaCollege of Life Sciences, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaPrimula poissonii (Primulaceae) is a perennial herb, widely distributed in the Hengduan Mountain region of Southwest China. In this study, Roche 454 pyrosequencing was used to isolate microsatellite markers. A total of 4528 unique sequences were identified from 68,070 unique reads. Of these, eighty-seven microsatellite loci were screened for utility using two criteria: successful PCR amplification and variation of these loci within three wild P. poissonii populations. Twenty loci were successfully amplified and exhibited polymorphic alleles. The number of observed alleles ranged from 1 to 9 with an average of 3.5. The observed and expected heterozygosities ranged from 0.087 to 1.000 and from 0.124 to 0.828, respectively. Among these SSR loci, only the P69 locus could not be cross-amplified successfully in two closely related species P. wilsonii and P. anisodora. The microsatellite loci developed in this study will be useful for studying genetic diversity and speciation events between P. poissonii and closely related Primula species.http://www.mdpi.com/1420-3049/21/5/536Primula poissonii454 pyrosequencingSSRgenetic diversityspeciation eventstransferability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yun-Jiao Liu Cai-Yun Zhang Gang Hao Xue-Jun Ge Hai-Fei Yan |
spellingShingle |
Yun-Jiao Liu Cai-Yun Zhang Gang Hao Xue-Jun Ge Hai-Fei Yan Characterization of Novel Microsatellite Loci for Primula poissonii (Primulaceae) Using High-Throughput Sequencing Technology Molecules Primula poissonii 454 pyrosequencing SSR genetic diversity speciation events transferability |
author_facet |
Yun-Jiao Liu Cai-Yun Zhang Gang Hao Xue-Jun Ge Hai-Fei Yan |
author_sort |
Yun-Jiao Liu |
title |
Characterization of Novel Microsatellite Loci for Primula poissonii (Primulaceae) Using High-Throughput Sequencing Technology |
title_short |
Characterization of Novel Microsatellite Loci for Primula poissonii (Primulaceae) Using High-Throughput Sequencing Technology |
title_full |
Characterization of Novel Microsatellite Loci for Primula poissonii (Primulaceae) Using High-Throughput Sequencing Technology |
title_fullStr |
Characterization of Novel Microsatellite Loci for Primula poissonii (Primulaceae) Using High-Throughput Sequencing Technology |
title_full_unstemmed |
Characterization of Novel Microsatellite Loci for Primula poissonii (Primulaceae) Using High-Throughput Sequencing Technology |
title_sort |
characterization of novel microsatellite loci for primula poissonii (primulaceae) using high-throughput sequencing technology |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2016-05-01 |
description |
Primula poissonii (Primulaceae) is a perennial herb, widely distributed in the Hengduan Mountain region of Southwest China. In this study, Roche 454 pyrosequencing was used to isolate microsatellite markers. A total of 4528 unique sequences were identified from 68,070 unique reads. Of these, eighty-seven microsatellite loci were screened for utility using two criteria: successful PCR amplification and variation of these loci within three wild P. poissonii populations. Twenty loci were successfully amplified and exhibited polymorphic alleles. The number of observed alleles ranged from 1 to 9 with an average of 3.5. The observed and expected heterozygosities ranged from 0.087 to 1.000 and from 0.124 to 0.828, respectively. Among these SSR loci, only the P69 locus could not be cross-amplified successfully in two closely related species P. wilsonii and P. anisodora. The microsatellite loci developed in this study will be useful for studying genetic diversity and speciation events between P. poissonii and closely related Primula species. |
topic |
Primula poissonii 454 pyrosequencing SSR genetic diversity speciation events transferability |
url |
http://www.mdpi.com/1420-3049/21/5/536 |
work_keys_str_mv |
AT yunjiaoliu characterizationofnovelmicrosatellitelociforprimulapoissoniiprimulaceaeusinghighthroughputsequencingtechnology AT caiyunzhang characterizationofnovelmicrosatellitelociforprimulapoissoniiprimulaceaeusinghighthroughputsequencingtechnology AT ganghao characterizationofnovelmicrosatellitelociforprimulapoissoniiprimulaceaeusinghighthroughputsequencingtechnology AT xuejunge characterizationofnovelmicrosatellitelociforprimulapoissoniiprimulaceaeusinghighthroughputsequencingtechnology AT haifeiyan characterizationofnovelmicrosatellitelociforprimulapoissoniiprimulaceaeusinghighthroughputsequencingtechnology |
_version_ |
1725781008716922880 |