Gas Chromatography-Mass Spectrometry Based Midgut Metabolomics Reveals the Metabolic Perturbations under NaF Stress in <i>Bombyx mori</i>
Fluoride tolerance is an important economic trait in sericulture, especially in some industrial development regions. Analyses of physiological changes involving structural damage to the insect body and molecular analyses of some related genes have focused on this area; however, the changes that occu...
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doaj-f6ff67b939ac45fe8301e51d1b807ab02020-11-24T22:09:55ZengMDPI AGInsects2075-44502019-12-011111710.3390/insects11010017insects11010017Gas Chromatography-Mass Spectrometry Based Midgut Metabolomics Reveals the Metabolic Perturbations under NaF Stress in <i>Bombyx mori</i>Gang Li0Xiao Zhang1Heying Qian2Mingzhu Liu3Guodong Zhao4Anying Xu5The Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaThe Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaThe Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaThe key Laboratory of silkworm and mulberry genetic improvement, Ministry of Agriculture, Chinese Academy of Agricultural Science, Zhenjiang 212018, ChinaThe Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaThe Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaFluoride tolerance is an important economic trait in sericulture, especially in some industrial development regions. Analyses of physiological changes involving structural damage to the insect body and molecular analyses of some related genes have focused on this area; however, the changes that occur at the metabolic level of silkworms after eating fluoride-contaminated mulberry leaves remain unclear. Here, metabonomic analysis was conducted using gas chromatography-mass spectrometry (GC-MS) to analyze the changes in midgut tissue after NaF stress using silkworm strains 733xin (susceptible stain) and T6 (strain resistant to fluoride), which were previously reported by our laboratory. Differential metabolomics analysis showed that both T6 and 733xin strains displayed complex responses after exposure to 200 mg/kg NaF. The purine metabolism and arginine and proline metabolic pathways of fluoride-tolerant strains reached significant levels, among which 3′-adenylic acid and hypoxanthine were significantly upregulated, whereas guanine, allantoic acid, xanthine, N-acetyl-L-glutamic acid, and pyruvate were significantly downregulated. These metabolic pathways may be related to the fluoride tolerance mechanism of NaF poisoning and tolerant strains.https://www.mdpi.com/2075-4450/11/1/17fluoride toleranceuntargeted metabolomicssilkworm midgutdifferential metabolitesmetabolic pathways |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gang Li Xiao Zhang Heying Qian Mingzhu Liu Guodong Zhao Anying Xu |
spellingShingle |
Gang Li Xiao Zhang Heying Qian Mingzhu Liu Guodong Zhao Anying Xu Gas Chromatography-Mass Spectrometry Based Midgut Metabolomics Reveals the Metabolic Perturbations under NaF Stress in <i>Bombyx mori</i> Insects fluoride tolerance untargeted metabolomics silkworm midgut differential metabolites metabolic pathways |
author_facet |
Gang Li Xiao Zhang Heying Qian Mingzhu Liu Guodong Zhao Anying Xu |
author_sort |
Gang Li |
title |
Gas Chromatography-Mass Spectrometry Based Midgut Metabolomics Reveals the Metabolic Perturbations under NaF Stress in <i>Bombyx mori</i> |
title_short |
Gas Chromatography-Mass Spectrometry Based Midgut Metabolomics Reveals the Metabolic Perturbations under NaF Stress in <i>Bombyx mori</i> |
title_full |
Gas Chromatography-Mass Spectrometry Based Midgut Metabolomics Reveals the Metabolic Perturbations under NaF Stress in <i>Bombyx mori</i> |
title_fullStr |
Gas Chromatography-Mass Spectrometry Based Midgut Metabolomics Reveals the Metabolic Perturbations under NaF Stress in <i>Bombyx mori</i> |
title_full_unstemmed |
Gas Chromatography-Mass Spectrometry Based Midgut Metabolomics Reveals the Metabolic Perturbations under NaF Stress in <i>Bombyx mori</i> |
title_sort |
gas chromatography-mass spectrometry based midgut metabolomics reveals the metabolic perturbations under naf stress in <i>bombyx mori</i> |
publisher |
MDPI AG |
series |
Insects |
issn |
2075-4450 |
publishDate |
2019-12-01 |
description |
Fluoride tolerance is an important economic trait in sericulture, especially in some industrial development regions. Analyses of physiological changes involving structural damage to the insect body and molecular analyses of some related genes have focused on this area; however, the changes that occur at the metabolic level of silkworms after eating fluoride-contaminated mulberry leaves remain unclear. Here, metabonomic analysis was conducted using gas chromatography-mass spectrometry (GC-MS) to analyze the changes in midgut tissue after NaF stress using silkworm strains 733xin (susceptible stain) and T6 (strain resistant to fluoride), which were previously reported by our laboratory. Differential metabolomics analysis showed that both T6 and 733xin strains displayed complex responses after exposure to 200 mg/kg NaF. The purine metabolism and arginine and proline metabolic pathways of fluoride-tolerant strains reached significant levels, among which 3′-adenylic acid and hypoxanthine were significantly upregulated, whereas guanine, allantoic acid, xanthine, N-acetyl-L-glutamic acid, and pyruvate were significantly downregulated. These metabolic pathways may be related to the fluoride tolerance mechanism of NaF poisoning and tolerant strains. |
topic |
fluoride tolerance untargeted metabolomics silkworm midgut differential metabolites metabolic pathways |
url |
https://www.mdpi.com/2075-4450/11/1/17 |
work_keys_str_mv |
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