Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB

Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB

Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the poultry industry worldwide. In this study, site-dire...

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Bibliographic Details
Main Authors: Sérgio P. Fernandes da Costa, Christos G. Savva, Monika Bokori-Brown, Claire E. Naylor, David S. Moss, Ajit K. Basak, Richard W. Titball
Format: Article
Language:English
Published: MDPI AG 2014-03-01
Series:Toxins
Subjects:
NetB
pore-forming toxin
Clostridium perfringens
necrotic enteritis
Online Access:http://www.mdpi.com/2072-6651/6/3/1049
Description
Summary:Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the poultry industry worldwide. In this study, site-directed mutagenesis was used to identify amino acids that play a role in NetB oligomerisation and pore-formation. NetB K41H showed significantly reduced toxicity towards LMH cells and human red blood cells relative to wild type toxin. NetB K41H was unable to oligomerise and form pores in liposomes. These findings suggest that NetB K41H could be developed as a genetic toxoid vaccine to protect against necrotic enteritis.
ISSN:2072-6651

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